Birth of live Spanish ibex (Capra pyrenaica hispanica) derived from artificial insemination with epididymal spermatozoa retrieved after death

Santiago-Moreno, J.; Toledano-Dı́az, A.; Pulido‐Pastor, Antonio; Gómez-Brunet, A.; López-Sebastián, A.

doi:10.1016/j.theriogenology.2005.11.012

Cited by 62 publications

(28 citation statements)

References 46 publications

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“…Nowadays concrete examples of application of post-mortem sperm recovery from the epididymis are available for conservation of endangered species [37] and in constructing semen banks [38][39][40]. Preservation of epididymal sperm from accidental dead or slaughtered animals has been reported in several domestic and wild species as boar [41], cattle [42,43], goat [44], ibex [45,46], red deer [47] and sheep [38,48]. However epididymal sperm extraction sometimes is not easily practicable in marginal areas, due to the lack of facilities and expertise near the farming areas of local breeds.…”

Section: Ex Situ Conservation Strategiesmentioning

confidence: 99%

Conservation of endangered animals: From biotechnologies to digital preservation

Pizzi¹,

Caroli²,

Landini³

et al. 2013

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In the recent years, the number of endangered animals, both referred to livestock and wild species, has grown enormously. The "livestock" term refers to animals domesticated for producing commodities for man such as food, fiber and draught. Livestock biodiversity is integral to our culture, history, environment, and economy. Thousands of livestock breeds have evolved over time to suit particular environments and farming systems. Conservation and analyses of these genetic resources rely on demographic characterization and correct breeding schemes. In addition, molecular genetic studies allow to identify and monitor the genetic diversity within and across breeds and to reconstruct their evolution history. The conservation of livestock variability is also a crucial element in order to preserve and valorise specific nutritional and nutraceutical properties of animal products. Efficient ex situ and in situ conservation strategies, as well as the creation of bio-banks and specific biotechnological and bioinformatics tools for genetic analyses and digital preservation, are obligatory requirements in order to implement an appropriate action for the conservation of animal biodiversity. The main issues concerning different species are summarised, with particular reference to the livestock biodiversity still existing. Some examples of ex situ conservation strategies, which mainly refer to cryoconservation of semen, ova, embryos or tissues, developed in Italy, are presented, and the different actions in defense of Animal Genetic Resources (AnGR) developed within the European Community are illustrated. Interestingly, the same strategies for biological and digital analyses and preservation of livestock biodiversity can be exported to wild endangered animals in order to plan a correct conservation and repopulation of the species. Furthermore, the European Union has set up the guidelines to safeguard the biodiversity and to combat the extinction of animal species, and has made the protection of biodiversity and ecosystems one of the main objectives of the Sixth Environment Action Programme.

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Section: Ex Situ Conservation Strategiesmentioning

confidence: 99%

Conservation of endangered animals: From biotechnologies to digital preservation

Pizzi¹,

Caroli²,

Landini³

et al. 2013

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show abstract

“…Nowadays, examples of application of postmortem sperm collection from the epididymis for the conservation of endangered species as in ibex (Santiago-Moreno et al, 2006) and in constructing semen banks (Ehling et al, 2006;Bagirov et al, 2009;León-Quinto et al, 2009) are reported. However, sometimes, epididymal sperm extraction is not easily practicable in marginal regions, owing to the lack of facilities and expertise near the farming areas of local breeds.…”

Section: Introductionmentioning

confidence: 99%

Effect of testicle postmortem storage on goat frozen-thawed epididymal sperm quality as a tool to improve genebanking in local breeds

Turri

Madeddu

Gliozzi

et al. 2014

Animal

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The interest to develop assisted reproductive technologies and cryobanking for farm animal genetic resource conservation has recently increased. However, cryopreservation for ex-situ management of genetic diversity sometimes is not routinely feasible, owing to the lack of facilities (AI centres, laboratories) and expertise near the local breed farming area. In these cases, epididymal sperm obtained from slaughtered or castrated animals, associated with the possibility of managing rather long periods between animal death, sperm recovery and freezing, would increase the opportunities to create semen storages. This investigation addresses the pre-freeze/post-thaw quality of goat epididymal sperm as a function of testicle storage temperature (environment or +5°C) and time elapsed between animal's death and sperm recovery (0, 24, 48, 72 h) to establish the optimal protocols for the recovery and cryopreservation of epididymal sperm in this species. Testicles of 50 mature bucks collected at the abattoir were divided in two groups: half of the testicles (n = 50) were transported to the laboratory at environment temperature (E), whereas the remaining half (n = 50) at a refrigeration temperature (R) of +5°C. In the two groups (E) and (R), one testicle from each pair was processed after slaughter forming the time 0 groups (0E and 0R). The contralateral testicle was processed after 24, 48 or 72 h of storage, at the corresponding temperature. Sperm motility and kinetic parameters, viability and morphology were assessed in pre-freeze and post-thaw samples. Until 48 h postmortem, both E and R temperatures are able to maintain good pre-freeze epididymal sperm quality. After 48 h postmortem, R temperature is fundamental to reduce epididymal sperm quality decay in pre-freeze samples. Moreover, testicle refrigeration also has a positive impact on post-thaw samples, allowing a lower decline through time considering total motility, kinetics parameters, sperm viability and sperm abnormalities. Therefore, when sperm cryopreservation is not immediately practicable, goat testicles should be transported and stored at 5°C up to a maximum of 48 h postmortem to ensure an acceptable sperm quality.

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“…Epididymal sperm collection and spermatozoa cryopreservation were done according to previous methodologies utilized in our laboratory (Santiago-Moreno et al, 2006a). Briefly, cauda epididymides were isolated from testes and then sperm were collected from the epididymides by performing small cuts in the tubules with a scalpel, and then collecting the fluid emerging from tubules.…”

Section: Introductionmentioning

confidence: 99%

“…The rationale behind the injection of progesterone in an oil vehicle was that it would lead to a less variable appearance of behavioral estrus, narrower synchronization of the ovulatory response (López- , and better quality of induced corpora lutea (Oldham et al, 1985). This protocol resulted in a higher fertility rate than synchronization methods based on intravaginal progestagens and eCG administration (25.0% vs 16.7%; Santiago-Moreno et al, 2006a). Furthermore, the advantages of this «simplified» method were that vaginal manipulation (and potentially vaginitis) was avoided and the deleterious effects derived from handling stress were minimized.…”

Section: Introductionmentioning

confidence: 99%

“…Previous studies have reported success in collecting viable epididymal spermatozoa from dead Spanish ibex males and subsequently artificially inseminating ibex females synchronized by inserting intravaginal progestagens for 11 days in conjunction with injections of equine chorionic gonadotropin (eCG) and cloprostenol two days before progestagen withdrawal (Santiago-Moreno et al, 2006a …”

mentioning

confidence: 99%

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Short communication. Synchronization of ovulation and artificial insemination protocol for Spanish ibex (Capra pyrenaica) based in progesterone and cloprostenol

Santiago-Moreno

Toledano-Dı́az

Gómez-Brunet

et al. 2008

Span. j. agric. res.

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The Spanish ibex (Capra pyrenaica) is an Iberian mountain ungulate that is listed as a threatened species, and thus is an ideal candidate for reproductive technologies that might enhance breeding programmes in this species. Previous studies have reported success in collecting viable epididymal spermatozoa from dead Spanish ibex males and subsequently artificially inseminating ibex females synchronized by inserting intravaginal progestagens for 11 days in conjunction with injections of equine chorionic gonadotropin (eCG) and cloprostenol two days before progestagen withdrawal (Santiago-Moreno et al., 2006a AbstractThis study describes the effectiveness of a method to synchronize ovulation in Spanish ibex for artificial insemination, based on one previously described for use in domestic goats. Estrus and ovulation were synchronised in eight adult ibex females (aged 3-5 yr old) during the breeding season (January) by i.m. injection of 25 mg of progesterone in olive oil plus an 100 µg i.m. of cloprostenol on day 0 followed by a single dose of 100 µg cloprostenol i.m. 9 days later (day 10). Ibexes were inseminated by laparoscopy with 200 × 10 6 spermatozoa at 52 h after the second cloprostenol injection. Two of the eight treated ibex (25%) conceived and carried their pregnancies to term. The protocol described here minimizes animal handling (in contrast to synchronization methods utilizing intravaginal progestins and equine chorionic gonadotropin injections), which greatly facilitates the application of reproductive technologies in Spanish ibex captive breeding programs.

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Birth of live Spanish ibex (Capra pyrenaica hispanica) derived from artificial insemination with epididymal spermatozoa retrieved after death

Cited by 62 publications

References 46 publications

Conservation of endangered animals: From biotechnologies to digital preservation

Conservation of endangered animals: From biotechnologies to digital preservation

Effect of testicle postmortem storage on goat frozen-thawed epididymal sperm quality as a tool to improve genebanking in local breeds

Short communication. Synchronization of ovulation and artificial insemination protocol for Spanish ibex (Capra pyrenaica) based in progesterone and cloprostenol

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