Birth of neural progenitors during the embryonic period of sexual differentiation in the Japanese quail brain

Bardet, Sylvia M.; Mouriec, Karen; Balthazart, Jacques

doi:10.1002/cne.23153

Cited by 14 publications

(36 citation statements)

References 112 publications

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“…In juvenile quails, very few PCNA-ir cells were observed in the mesopallium and caudal part of the nidopallium adjacent to the lateral ventricles. Similarly, Bardet et al (2012) reported the presence of a few PCNA and BrdU labelled cells in the quail pallium and concluded that these could be slow cycling progenitors. Alternatively, the presence of PCNA-ir cells in the parenchyma may not be related to cell proliferation but to DNA repair in mature neurons as proposed by Essers et al (2005).…”

Section: Age-related Changes In Pcna and DCX Expression In Different mentioning

confidence: 92%

“…In galliformes such as quails and chicks, neurogenesis starts at about 16-30% of hatching time and is presumed to be complete for the medulla by 30%, the tectum by 50% and the telencephalon by 50% of hatching time (Mezey et al 2012). However Bardet et al (2012) reported that neurons in most brain areas in the quail except for the telencephalon and the cerebellum are born and become post-mitotic before embryonic day 6 (E6). Even if neurogenesis persists throughout life in the telencephalon of adult birds, the bulk of neurons in this part of the brain are produced before E6 in quails (Bardet et al 2012) and E7-E9 in chicks (Tsai et al 1981).…”

Section: Ontogenetic Considerationsmentioning

confidence: 99%

“…However Bardet et al (2012) reported that neurons in most brain areas in the quail except for the telencephalon and the cerebellum are born and become post-mitotic before embryonic day 6 (E6). Even if neurogenesis persists throughout life in the telencephalon of adult birds, the bulk of neurons in this part of the brain are produced before E6 in quails (Bardet et al 2012) and E7-E9 in chicks (Tsai et al 1981). Our results reveal that the post hatch development of higher order brain areas such as the telencephalon and cerebellum is protracted in precocial birds in a similar way as observed in other birds and mammals.…”

Section: Ontogenetic Considerationsmentioning

confidence: 99%

“…However, songbirds constitute only about 2% of all avian species (Ling et al 1997). Furthermore, previous studies have revealed that the neurogenesis observed in the telencephalon is not only specific to songbirds but also occurs in other avian species belonging to other orders such as ring doves (Ling et al 1997, Ling andCheng 1995), pigeons (Melleu et al 2013, Meskenaite et al 2016) chicken (Mezey et al 2012, Nikolakopoulou et al 2006a) and quails (Balthazart et al 2010, Bardet et al 2012, Mouriec and Balthazart 2013, Nikolakopoulou et al 2006b). …”

Section: Introductionmentioning

confidence: 99%

“…The majority of neurogenesis studies in quails focussed on the sex steroid sensitive areas like the POM (Bardet et al 2012), intermediate medial mesopallium and lateral septal nucleus (Nikolakopoulou et al 2006b) with little attention given to neurogenesis in other quail brain areas like the telencephalon.…”

Section: Introductionmentioning

confidence: 99%

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Changes in neurogenesis with post-hatching age in the male Japanese quail (Cortunix japonica) brain

Nkomozepi¹,

Mazengenya²,

Ihunwo³

2018

Acta Neurobiologiae Experimentalis

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Most avian neurogenesis studies have previously focused on the song control system and little attention has been given to non-song birds. The objective of this study was to assess changes in neurogenesis associated with post-hatching age (3-12 weeks) in the Japanese quail brain using proliferating cell nuclear antigen (PCNA) and doublecortin (DCX) immunohistochemistry. PCNA-immunoreactive (ir) cells were observed mainly in the olfactory bulb ventricular zone, telencephalic ventricular zones and cerebellum. Fewer PCNA-ir cells were also observed in the hypothalamus, thalamus and bed nucleus of the stria terminalis. In telencephalic ventricular zones, PCNA-ir cells were concentrated ventrally and dorsally adjacent to the mesopallium and medial striatum, respectively. DCX-ir cells were observed in the olfactory bulb, telencephalon and cerebellum. Furthermore, DCX-ir cells were scattered throughout the pallium except in the entopallium and arcopallium, septal nuclei and striatum. Fewer DCX-ir cells were also observed in the hippocampus and bed nucleus of stria terminalis. The density of PCNA-ir cells and DCX-ir cells in all brain areas declined with post-hatching age. In conclusion, cell proliferation appears to be restricted to the ventricular zones whereas neuronal recruitment is more widespread in the olfactory bulb, telencephalon and cerebellum. Postnatal neuronal incorporation appears to be absent in the diencephalon and mesencephalon.

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Section: Age-related Changes In Pcna and DCX Expression In Different mentioning

confidence: 92%

Section: Ontogenetic Considerationsmentioning

confidence: 99%

Section: Ontogenetic Considerationsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Changes in neurogenesis with post-hatching age in the male Japanese quail (Cortunix japonica) brain

Nkomozepi¹,

Mazengenya²,

Ihunwo³

2018

Acta Neurobiologiae Experimentalis

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Endogenous versus exogenous markers of adult neurogenesis in canaries and other birds: Advantages and disadvantages

Balthazart

Ball

2014

J of Comparative Neurology

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Although the existence of newborn neurons had originally been suggested, but not broadly accepted, based on studies in adult rodent brains, the presence of an active neurogenesis process in adult homoeothermic vertebrates was first firmly established in songbirds. Adult neurogenesis was initially studied with the tritiated thymidine technique, later replaced by the injection and detection of the marker of DNA replication 5-bromo-2′-deoxyuridine (BrdU). More recently, various endogenous markers were used to identify young neurons or cycling neuronal progenitors. We review here the respective advantages and pitfalls of these different approaches in birds, with specific reference to the microtubule-associated protein, doublecortin (DCX), that has been extensively used to identify young newly born neurons in adult brains. All these techniques of course have limitations. Exogenous markers label cells replicating their DNA only during a brief period and it is difficult to select injection doses that would exhaustively label all these cells without inducing DNA damage that will also result in some form of labeling during repair. On the other hand, specificity of endogenous markers is difficult to establish due to problems related to the specificity of antibodies (these problems can be, but are not always, addressed) and more importantly because it is difficult, if not impossible, to prove that a given marker exhaustively and specifically labels a given cell population. Despite these potential limitations, these endogenous markers and DCX staining in particular clearly represent a useful approach to the detailed study of neurogenesis especially when combined with other techniques such as BrdU.

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Neurogenesis and the development of neural sex differences in vocal control regions of songbirds

Diez

Carfagnini

et al. 2021

J of Comparative Neurology

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The brain regions that control the learning and production of song and other learned vocalizations in songbirds exhibit some of the largest sex differences in the brain known in vertebrates and are associated with sex differences in singing behavior.Song learning takes place through multiple stages: an early sensory phase when song models are memorized, followed by a sensorimotor phase in which auditory feedback is used to modify song output through subsong, plastic song, to adult crystalized song. However, how patterns of neurogenesis in these brain regions change through these learning stages, and differ between the sexes, is little explored. We collected brains from 63 young male and female zebra finches (Taeniopygia guttata) over four stages of song learning. Using neurogenesis markers for cell division (proliferating cell nuclear antigen), neuron migration (doublecortin), and mature neurons (neuronspecific nuclear protein), we demonstrate that there are sex-specific changes in neurogenesis over song development that differ between the caudal motor pathway and anterior forebrain pathway of the vocal control circuit. In many of these regions, sex differences emerged very early in development, by 25 days post hatch, at the beginning of song learning. The emergence of sex differences in other components of the system was more gradual and had specific trajectories depending on the brain region and its function. In conclusion, we found that sex differences occurred early and continued during song learning. Moreover, transitions from the different phases of song development do not seem to depend on large changes in neurogenesis in the vocal control areas measured.

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Birth of neural progenitors during the embryonic period of sexual differentiation in the Japanese quail brain

Cited by 14 publications

References 112 publications

Changes in neurogenesis with post-hatching age in the male Japanese quail (Cortunix japonica) brain

Changes in neurogenesis with post-hatching age in the male Japanese quail (Cortunix japonica) brain

Endogenous versus exogenous markers of adult neurogenesis in canaries and other birds: Advantages and disadvantages

Neurogenesis and the development of neural sex differences in vocal control regions of songbirds

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