Birth of offspring following artificial insemination in the common marmoset,Callithrix jacchus

Morrell, Jane M.; Nowshari, M.A.; Rosenbusch, J P; Nayudu, P.L.; Hodges, J. K.

doi:10.1002/(sici)1098-2345(1997)41:1<37::aid-ajp3>3.0.co;2-0

Cited by 18 publications

(6 citation statements)

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“…Artificial insemination techniques have been used recently in NHP to manage captive colonies and propagate endangered or valuable founder animals [25]. Conception rates of the VW, EE, and CE methods are reportedly 100%, 16.7%, and 33.3%, respectively [14][15][16]. In contrast, our conception rate using the PVS-SU method was 57.1%, which was higher than that of EE and CE but lower than that of VW.…”

Section: Discussionmentioning

confidence: 92%

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Development of a modified artificial insemination technique combining penile vibration stimulation and the swim-up method in the common marmoset

2015

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Section: Discussionmentioning

confidence: 92%

“…In addition, four semen collection methods have been reported, including vaginal washing (VW) after copulation [3], electroejaculation (EE) [10], cauda epididymis (CE) [11], and penile vibratory stimulation (PVS) [12,13]. Vaginal washing, EE, and CE have been used for AI [14][15][16]. Those procedures have advantages and disadvantages.…”

Section: Introductionmentioning

confidence: 99%

Development of a modified artificial insemination technique combining penile vibration stimulation and the swim-up method in the common marmoset

2015

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“…Several approaches for marmoset sperm collection have been reported, as outlined below. Epididymal sperm can be collected from surgically dissected epididymis (Morrell et al, 1997). This approach is invasive and not repeatable.…”

Section: Introductionmentioning

confidence: 99%

Artificial insemination in common marmosets using sperm collected by penile vibratory stimulation

Ishibashi¹,

Motohashi²

2013

Neotrop. Primates

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“…While ejaculated sperm is recovered from live animals, epididymides and testes are potential major sources of male gametes in animals that die unexpectedly [8]. Live births have been produced using cryopreserved epididymal sperm in many species [10,15]. In addition, intracytoplasmic sperm injection (ICSI) with testicular sperm was successful in the human [6].…”

mentioning

confidence: 99%

A Case Report Concerning Male Gametes Rescued from a Siamese Eld’s Deer (<i>Rucervus eldii siamensis</i>): Post-Thawed Testicular and Epididymal Sperm Quality and Heterologous Zona Pellucida Binding Ability

Thuwanut

Thongphakdee

Sommanustweechai

et al. 2013

The Journal of Veterinary Medical Science

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ABSTRACT. In the present study, the quality of frozen-thawed epididymal and testicular sperm recovered from a Siamese Eld's deer was examined. The epididymal sperm quality was assessed in fresh, cold-stored at 4°C and frozen-thawed samples. Zona binding ability of the frozen-thawed epididymal samples with Burmese Eld's deer oocytes was also evaluated. Testicular sperm extracted from tissues frozen at −80 or −196°C for one month were examined for membrane and DNA integrity. Epididymal sperm retained their quality for up to 24 hr of cold storage at 4°C. The percentages of sperm motility, intact membrane, intact acrosome and intact DNA were 30, 46.5, 27 and 89.5% in the frozen and thawed epididymal sperm, and the average ability to bind with oocytes was 92.5 ± 64 sperm/oocytes. Around 70% of the sperm extracted from testicular tissues cryopreserved at −196 and −80°C for one month showed an intact membrane. In conclusion, epididymal and testicular sperm survived for more than 13 hr post-mortem. Furthermore, cold storage at 4°C and cryopreservation at −196 and −80°C maintain the quality of epididymal and testicular sperm. This study represents a model for male gamete rescue in endangered Eld's deer. KEY WORDS: Siamese Eld's deer, sperm collection post mortem, sperm cryopreservation.doi: 10.1292/jvms.11-0491; J. Vet. Med. Sci. 75(1): 123-125, 2013 Cryotechnology of genetic resources is notable as a potential tool to conserve germplasm of endangered wild species [5]. While ejaculated sperm is recovered from live animals, epididymides and testes are potential major sources of male gametes in animals that die unexpectedly [8]. Live births have been produced using cryopreserved epididymal sperm in many species [10,15]. In addition, intracytoplasmic sperm injection (ICSI) with testicular sperm was successful in the human [6]. Siamese Eld's deer (Rucervus eldii siamensis) are native to Thailand but have been extirpated in the wild and are found only in captivity [12]. Although production of offspring after intrauterine insemination with frozen-thawed ejaculated Burmese Eld's deer sperm was reported [9], there are no studies of Siamese Eld's deer reproductive biology. The aim of this study was to evaluate and cryopreserve male gametes recovered from the epididymis and testes of a Siamese Eld's deer that died in captivity.A two-year-old male Siamese Eld's deer maintained at Dusit Zoo, Bangkok, Thailand, unexpectedly died of an unknown cause at 11:00 pm on January 18, 2009, and was kept in a refrigerator until the next day (10:00 am). The right testis with epididymis was removed, stored in phosphate buffered saline at 4°C and transported to a laboratory within 2 hr. Epididymal sperm was collected by placing the caudal epididymis into 10 ml pre-warmed phosphate buffer saline (PBS), cut into small pieces and incubated at 37°C for 10 min. After epididymal tissues were removed, sperm samples were evaluated for sperm concentration, total number, motility (20× magnification with a phase contrast microscope), morphology (William's...

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Birth of offspring following artificial insemination in the common marmoset,Callithrix jacchus

Cited by 18 publications

References 20 publications

Development of a modified artificial insemination technique combining penile vibration stimulation and the swim-up method in the common marmoset

Development of a modified artificial insemination technique combining penile vibration stimulation and the swim-up method in the common marmoset

Artificial insemination in common marmosets using sperm collected by penile vibratory stimulation

A Case Report Concerning Male Gametes Rescued from a Siamese Eld’s Deer (<i>Rucervus eldii siamensis</i>): Post-Thawed Testicular and Epididymal Sperm Quality and Heterologous Zona Pellucida Binding Ability

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