Birth of piglets preselected for gender following in vitro fertilization of in vitro matured pig oocytes by X and Y chromosome bearing spermatozoa sorted by high speed flow cytometry

Abeydeera, Lalantha R.; Johnson, Lawrence A.; Welch, G. Rickey; Wang, W.H; Boquest, Andrew C.; Cantley, T.C.; Rieke, August; Day, Billy N.

doi:10.1016/s0093-691x(98)00201-5

Cited by 73 publications

(31 citation statements)

References 14 publications

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“…This technique provides a useful semen-delivery avenue needed to bring flow sorted semen to producers. In IVF of porcine oocytes, a study using separated sperm after highspeed sorting demonstrated the effectiveness of using the sorted sperm to produce sex preselected offspring [64]. High-speed sorting of bull sperm can provide separated X-or Y-sperm for AI in the conventional manner.…”

Section: Production Of Sex Preselected Offspring With Flow Sorted X-amentioning

confidence: 99%

Sex Preselection in Bovine by Separation of X- and Y-Chromosome Bearing Spermatozoa

Hamano

2007

Journal of Reproduction and Development

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Abstract. Flow cytometrically-sorted sperm has been involved in the production of sex preselected offspring. More than 30,000 bovine offspring have been produced using AI and other means using spermatozoa separated by flow cytometer. Flow cytometric sperm sorting based on differences in their DNA content is the best method for separation of X-and Y-chromosome bearing spermatozoa. At first, flow cytometers were modified for DNA confirmation and sorting of sperm with high resolution. The beveled insertion needle can regulate orientation of flat-shaped bull sperm heads. The forward fluorescence detector is essential for measuring the DNA content of sperm. Recently, high-speed sperm sorting with orienting nozzles has resulted in production of 90% pure X-and Ysperm at rate of 15-20 million sperm per hour. Application of this new technique will enable conduct of more conventional technologies for both artificial insemination and cryopreservation in the bovine and in other farm animals using X-or Y-sperm.

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Section: Production Of Sex Preselected Offspring With Flow Sorted X-amentioning

confidence: 99%

Sex Preselection in Bovine by Separation of X- and Y-Chromosome Bearing Spermatozoa

Hamano

2007

Journal of Reproduction and Development

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“…It needs to be established whether taurine and hypotaurine in NCSU-23 should be replaced with sorbitol in NCSU-37 or whether it would perhaps be better to use all three supplements. Both the former (Rath et al 1995;Abeydeera et al 1998c) and latter (Petters and Wells 1993;Funahashi et al 1996) compounds have been ascribed important physiological roles and may, therefore, exert synergistic effects when included in a medium in the appropriate ratios. Taurine and hypotaurine act as osmoregulators and pH stabilizers and the supplementation of culture media with taurine has been shown to enhance the development of porcine embryos (Petters and Reed 1991;Petters and Wells 1993), probably owing to a protective effect against peroxidative damage (Guerin et al 1995).…”

Section: Embryo Culturementioning

confidence: 99%

In vitro production of pig embryos: a point of view

Coy

Romar

2002

Reprod. Fertil. Dev.

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Porcine embryos have become raw materials for different programmes of reproductive biotechnology and the in vitro production of embryos has some advantages over in vivo production in gene transfer programmes and for xenotransplantation. Despite this promising future, several problems limit the success of the in vitro production (IVP) of viable porcine embryos. Porcine IVP has not been fully developed because of several problems associated with different techniques, such as incomplete final maturation status after in vitro maturation, a high incidence of polyspermy after in vitro fertilization and a low development rate and poor quality of blastocysts at the end of culture. The results could be improved with studies comparing in vivo and in vitro conditions, standardization of techniques for sperm processing, testing new additives in the culture media and developing intracytoplasmic sperm injection procedures. The first objective of the present article is to summarize the main studies published on the subject. Second, we provide a guide for researchers starting work on the IVP of pig embryos, making special mention of first papers and the most recent achievements for each of the different techniques. Third, we provide suggestions for future experiments designed to improve the results of each technique.

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“…In the recent past, encouraging progress has been made in in vitro culture (IVC) of porcine embryos, and not only has IVC been used to obtain offspring (Abeydeera et al 1998;Kikuchi et al 2002;Yoshioka et al 2012), but this technology has also enabled successes in pig somatic cloning and transgenic cloning (Betthauser et al 2000;Phelps et al 2003). In the recent past, encouraging progress has been made in in vitro culture (IVC) of porcine embryos, and not only has IVC been used to obtain offspring (Abeydeera et al 1998;Kikuchi et al 2002;Yoshioka et al 2012), but this technology has also enabled successes in pig somatic cloning and transgenic cloning (Betthauser et al 2000;Phelps et al 2003).…”

Section: Introductionmentioning

confidence: 99%

Effect of follistatin on pre‐implantational development of pig parthenogenetic embryos

Liu

Chen

et al. 2017

Animal Science Journal

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The present study was designed to explore effects of follistatin (FST) on pre-implantational development of parthenogenetically activated embryos (PAEs) in pigs. First, we investigated the FST messenger RNA expression level and dynamic FST protein expression patterns in porcine oocytes and PAEs. Then, PAEs were placed in embryo culture medium supplemented with 10 ng/mL of FST-288, FST-300, and FST-315. Next, PAEs were cultured with 0, 1, 10 and 100 ng/mL of FST-315 protein throughout the in vitro culture (IVC) duration. Further, 10 ng/mL of FST-300 was added from the start of IVC in which PAEs were treated for 30, 48 and 60 h. The results showed that 1 ng/mL FST-315 could significantly increase the total cell numbers of blastocyst and trophectoderm cell number in PAEs. Exogenous FST-300 supplementation could significantly promote the early cleavage divisions and improve the blastocyst formation rate of porcine embryos. FST-300 appeared to affect early embryonic development before activation of the embryonic genome. In all, the study confirmed for the first time that FST plays a role in promoting early embryonic development in pigs, which differed with different FST subtypes. FST-300 could facilitate the initial cleavage time and improve the blastocyst formation rate, and FST-315 could improve the blastocyst quality.

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Birth of piglets preselected for gender following in vitro fertilization of in vitro matured pig oocytes by X and Y chromosome bearing spermatozoa sorted by high speed flow cytometry

Cited by 73 publications

References 14 publications

Sex Preselection in Bovine by Separation of X- and Y-Chromosome Bearing Spermatozoa

Sex Preselection in Bovine by Separation of X- and Y-Chromosome Bearing Spermatozoa

In vitro production of pig embryos: a point of view

Effect of follistatin on pre‐implantational development of pig parthenogenetic embryos

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