2017
DOI: 10.1111/asj.12763
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Birth of puppies of predetermined sex after artificial insemination with a low number of sex‐sorted, frozen–thawed spermatozoa in field conditions

Abstract: The aim of this study was to evaluate fertility and sex ratios after artificial insemination in dogs under field conditions. Semen was cryopreserved as unsorted (control) or was separated into X- and Y-chromosome-bearing sperm using a cell sorter. Sixty female dogs were inseminated with frozen-thawed spermatozoa of 100 × 10 unsorted (a dose in practice) and 4 × 10 sorted (X and Y group, respectively). A total of 20 dogs became pregnant and 126 puppies were born from the three groups. The percentage of parturit… Show more

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Cited by 5 publications
(2 citation statements)
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“…An accuracy of 90% is ideal because a certain percentage of male offspring is necessary to maintain the breeding colony. Sorted frozen-thawed spermatozoa after surgical artificial insemination reportedly yielded pups with a significantly biased sex ratio [ 88 ]. In general, freezing and thawing procedures and cell sorting adversely affect sperm motility.…”
Section: Sexing Spermatozoamentioning
confidence: 99%
“…An accuracy of 90% is ideal because a certain percentage of male offspring is necessary to maintain the breeding colony. Sorted frozen-thawed spermatozoa after surgical artificial insemination reportedly yielded pups with a significantly biased sex ratio [ 88 ]. In general, freezing and thawing procedures and cell sorting adversely affect sperm motility.…”
Section: Sexing Spermatozoamentioning
confidence: 99%
“…To date, the sexed semen is used for cattle (Garner and Seidel, 2008;DeJarnette et al, 2011), rams (De Graaf et al, 2007, horses (Clulow et al, 2008;Buchanan et al, 2020), water buffalo (Lu et al, 2010), elk (Schenk and DeGrofft, 2003), boars (Johnson et al 2000), Western Lowland Gorilla (O'Brien et al, 2005, W hite-Tail deer (Kjelland et al, 2011), cats (Pope et al, 2009); bottlenose dolphins (O'Brien et al, 2009); Pigs (Johnson 1991, Martinez et al, 2001Rath et al, 2003), goats (Bathgate et al, 2013) and dogs (Wei et al, 2017) have resulted in the birth of desired sex.…”
Section: Flow Cytometry Techniquementioning
confidence: 99%