The present study was outlined to appraise the interrelation between heamatological, blood metabolic pro les and lactation stages in crossbred (CB) dairy cows in ANI. Healthy (n = 18) CB cows with age of 5-7 years were selected from cattle breeding farm, ICAR-CIARI, Port Blair, ANI. Heamatological pro les, serum biochemical pro les, oxidative stress markers and endocrinological pro les were measured at 15day interval from day 01 to 305 of lactation and divided into rst (day 01 to 90), second (day 91 to 180) and third (day 181 to 305) stage of lactation. Average milk yield (L) was not signi cantly differed among the stages of lactations; however, rst (8.42 ± 1.18) and second (9.67 ± 0.25) stages were had higher than third (7.82 ± 0.47) stage of lactation. Hematological pro les were not-differed signi cantly among the stages of lactation; however, these values were within the range of bovine species. Serum biochemical pro les such as glucose, triglyceride, total cholesterol, total protein and globulin were escalated signi cantly (p < 0.05) as stage of lactation advanced whereas albumin and creatinine were dwindled signi cantly (p < 0.05) as lactation stages progressed. Aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), calcium, phosphorous and magnesium were nonsigni cantly differed among the stages of lactation. Similarly, serum endocrinological pro les such as Triiodo thyronine (T3), Thyroxine (T4) were surged and prolactin and cortisol were diminished signi cantly (p < 0.05) as stages of lactation progressed. Total antioxidant capacity (TAC) was increased and oxidative stress marker (malondialdehyde; MDA) was decreased signi cantly (p < 0.05) as stage progressed. It is concluded that among different stages of lactation, rst and second stages are more stressful events as contrast to third stage of lactation in crossbred cattle in humid tropical island ecosystem of ANI.