Bisphenols disrupt differentiation of the pigmented cells during larval brain formation in the ascidian

Gomes, Isa D. L.; Gazo, Ievgeniia; Nabi, Dalileh; Besnardeau, Lydia; Hebras, Céline; McDougall, Alex; Dumollard, Rémi

doi:10.1016/j.aquatox.2019.105314

Cited by 20 publications

(14 citation statements)

References 46 publications

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“…However, these concentrations of pesticides induced reduction of at least three studied morphological parameters, but did not lead to the same phenotypes like genotoxicants. Our previous studies showed severe genotoxicity of BPA at 40 µM (Gomes et al, 2019b); however, a small number of embryos with micronuclei was detected already at 10 µM (Table 6). In contrast, atrazine did not induce any detectable DNA damage, and only two endpoints were affected.…”

Section: Phenotypes Of General Toxicants and Genotoxicantsmentioning

confidence: 83%

“…In order to ensure proper penetration of the studied compounds into eggs, they were dechorionated with 0.1% Trypsin (Sigma-Aldrich, T9201) in filtered sea water (FSW) for 60 min at 20°C, then transferred to fresh sea water and stored at 18°C until required. Sperm was collected by syringe aspiration from a sperm duct and was activated with high pH 9.2 sea water for 10-20 min, then used to fertilize dechorionated eggs (Gomes et al, 2019b; see full protocols on http://lbdv.obs-vlfr.fr/en/research/research_groups/ascidian-biocellgroup/projets/fertilization-and-meiosis/protocols.html). The embryos were reared in dark conditions to avoid photodegradation of chemicals.…”

Section: Animalsmentioning

confidence: 99%

“…Dechorionated eggs (approximately 1000 eggs) were mixed with activated sperm (10 6 /ml diluted in FSW) in the embryo culture medium for fertilization (Sardet et al, 2011;Gomes et al, 2019b).…”

Section: Experimental Designmentioning

confidence: 99%

“…Many phenotypes of EDCs exposure have been described in invertebrate species (Urushitani et al, 2018;Castro et al, 2007;le Maire et al, 2009;Nishikawa et al, 2004;Matsushima et al, 2013), but the mode-of-action of these EDCs remains mainly unclear. One approach to predict whether a xenobiotic might target a NR is to compare its phenotype against the phenotypes produced by a specific NR agonist/antagonist (Tohmé et al, 2014, Gomes et al, 2019b. If a phenotypic signature can be associated with a specific NR agonist/antagonist, then it can be potentially used as a biomarker of NR-signaling disruption.…”

Section: Introductionmentioning

confidence: 99%

“…The pesticides analyzed in the current study (lindane, atrazine, chlordane, chlorpyrifos), as well as BPA, are often referred to as EDCs (Mnif et al, 2011); however, their mechanism of action in marine invertebrates is unknown. Our previous work showed that several NRs (RAR, PXR/VDRα, PPAR, ROR, and ERR) are expressed in neurogenic regions of Phallusia mammillata embryos (Gomes et al, 2019a, Gomes et al, 2019b. We analyzed known agonists/antagonists of these NRs, such as UVI3003 (RXR antagonist), all-trans retinoic acid (RAR agonist), rifampicin and SR12813 (PXR agonists), SR1078 (ROR agonist), BADGE (PPAR agonist), diethylstilbestrol, and 4-hydrotamoxifen (ERR antagonists).…”

Section: Introductionmentioning

confidence: 99%

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High-content analysis of larval phenotypes for the screening of xenobiotic toxicity using Phallusia mammillata embryos

et al. 2021

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In recent years, pollution of surface waters with xenobiotic compounds became an issue of concern in society and has been the object of numerous studies. Most of these xenobiotic compounds are man-made molecules and some of them are qualified as endocrine disrupting chemicals (EDCs) when they interfere with hormones actions. Several studies have investigated the teratogenic impacts of EDCs in vertebrates (including marine vertebrates). However, the impact of such EDCs on marine invertebrates is much debated and still largely obscure. In addition, DNA-altering genotoxicants can induce embryonic malformations. The goal of this study is to develop a reliable and effective test for assessing toxicity of chemicals using embryos of the ascidian (Phallusia mammillata) in order to find phenotypic signatures associated with xenobiotics. We evaluated embryonic malformations with high-content analysis of larval phenotypes by scoring several quantitative and qualitative morphometric endpoints on a single image of Phallusia tadpole larvae with semi-automated image analysis. Using this approach we screened different classes of toxicants including genotoxicants, known or suspected EDCs and nuclear receptors (NRs) ligands. Manuscript FileClick here to view linked References 2The screen presented here reveals a specific phenotypic signature for ligands of retinoic acid receptor/retinoid X receptor. Analysis of larval morphology combined with DNA staining revealed that embryos with DNA aberrations displayed severe malformations affecting multiple aspects of embryonic development. In contrast EDCs exposure induced no or little DNA aberrations and affected mainly neural development. Therefore the ascidian embryo/larval assay presented here can allow to distinguish the type of teratogenicity induced by different classes of toxicants.

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Section: Phenotypes Of General Toxicants and Genotoxicantsmentioning

confidence: 83%

Section: Animalsmentioning

confidence: 99%

Section: Experimental Designmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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High-content analysis of larval phenotypes for the screening of xenobiotic toxicity using Phallusia mammillata embryos

et al. 2021

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Effect of bisphenol A on the neurological system: a review update

Costa,

Cairrao

2023

Arch Toxicol

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Bisphenol A (BPA) is an endocrine-disrupting chemical (EDC) and one of the most produced synthetic compounds worldwide. BPA can be found in epoxy resins and polycarbonate plastics, which are frequently used in food storage and baby bottles. However, BPA can bind mainly to estrogen receptors, interfering with various neurologic functions, its use is a topic of significant concern. Nonetheless, the neurotoxicity of BPA has not been fully understood despite numerous investigations on its disruptive effects. Therefore, this review aims to highlight the most recent studies on the implications of BPA on the neurologic system. Our findings suggest that BPA exposure impairs various structural and molecular brain changes, promoting oxidative stress, changing expression levels of several crucial genes and proteins, destructive effects on neurotransmitters, excitotoxicity and neuroinflammation, damaged blood–brain barrier function, neuronal damage, apoptosis effects, disruption of intracellular Ca2+ homeostasis, increase in reactive oxygen species, promoted apoptosis and intracellular lactate dehydrogenase release, a decrease of axon length, microglial DNA damage, astrogliosis, and significantly reduced myelination. Moreover, BPA exposure increases the risk of developing neurologic diseases, including neurovascular (e.g. stroke) and neurodegenerative (e.g. Alzheimer’s and Parkinson’s) diseases. Furthermore, epidemiological studies showed that the adverse effects of BPA on neurodevelopment in children contributed to the emergence of serious neurological diseases like attention-deficit/hyperactivity disorder (ADHD), autism spectrum disorder (ASD), depression, emotional problems, anxiety, and cognitive disorders. In summary, BPA exposure compromises human health, promoting the development and progression of neurologic disorders. More research is required to fully understand how BPA-induced neurotoxicity affects human health.

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Effect of bisphenol A on the ovarian expressions of estrogen-related receptor gene and protein in queen honey bee (Apis mellifera)

Ouyang,

Zhu,

Wang

et al. 2023

Apidologie

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Bisphenols disrupt differentiation of the pigmented cells during larval brain formation in the ascidian

Cited by 20 publications

References 46 publications

High-content analysis of larval phenotypes for the screening of xenobiotic toxicity using Phallusia mammillata embryos

High-content analysis of larval phenotypes for the screening of xenobiotic toxicity using Phallusia mammillata embryos

Effect of bisphenol A on the neurological system: a review update

Effect of bisphenol A on the ovarian expressions of estrogen-related receptor gene and protein in queen honey bee (Apis mellifera)

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