Bisphosphonates offer protection against prosthetic joint infections caused by Staphylococcus aureus and Staphylococcus epidermidis biofilms

Hiltunen, Anna; Vuorela, Pia; Fallarero, Adyary

doi:10.1016/j.jddst.2017.06.002

Cited by 5 publications

(3 citation statements)

References 20 publications

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“…A wide range of different implant materials are used in orthopedics. Despite the intensive research currently being performed on different technologies for incorporating antimicrobial agents [22][23][24][25][26][27][28][29][30][31][32], only a few studies investigating biofilm formation on different clinically relevant implantation materials have been published [33][34][35][36][37][38]. The dynamic changes associated with biofilm growth [39] make biofilm eradication from clinical materials even more challenging.…”

Section: Introductionmentioning

confidence: 99%

Structural and Functional Dynamics of Staphylococcus aureus Biofilms and Biofilm Matrix Proteins on Different Clinical Materials

et al. 2019

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Medical device-associated staphylococcal infections are a common and challenging problem. However, detailed knowledge of staphylococcal biofilm dynamics on clinically relevant surfaces is still limited. In the present study, biofilm formation of the Staphylococcus aureus ATCC 25923 strain was studied on clinically relevant materials—borosilicate glass, plexiglass, hydroxyapatite, titanium and polystyrene—at 18, 42 and 66 h. Materials with the highest surface roughness and porosity (hydroxyapatite and plexiglass) did not promote biofilm formation as efficiently as some other selected materials. Matrix-associated poly-N-acetyl-β-(1-6)-glucosamine (PNAG) was considered important in young (18 h) biofilms, whereas proteins appeared to play a more important role at later stages of biofilm development. A total of 460 proteins were identified from biofilm matrices formed on the indicated materials and time points—from which, 66 proteins were proposed to form the core surfaceome. At 18 h, the appearance of several r-proteins and glycolytic adhesive moonlighters, possibly via an autolysin (AtlA)-mediated release, was demonstrated in all materials, whereas classical surface adhesins, resistance- and virulence-associated proteins displayed greater variation in their abundances depending on the used material. Hydroxyapatite-associated biofilms were more susceptible to antibiotics than biofilms formed on titanium, but no clear correlation between the tolerance and biofilm age was observed. Thus, other factors, possibly the adhesive moonlighters, could have contributed to the observed chemotolerant phenotype. In addition, a protein-dependent matrix network was observed to be already well-established at the 18 h time point. To the best of our knowledge, this is among the first studies shedding light into matrix-associated surfaceomes of S. aureus biofilms grown on different clinically relevant materials and at different time points.

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Section: Introductionmentioning

confidence: 99%

Structural and Functional Dynamics of Staphylococcus aureus Biofilms and Biofilm Matrix Proteins on Different Clinical Materials

et al. 2019

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“…The adherence of bacteria was evaluated by the static biofilm method with some modifications according to the protocol by Hiltunen et al [32]. A sterile Whatman filter paper (70 mm diameter, qualitative grade 2, GE Healthcare, Little Chalfont, U.K.) was placed on TSA plate (90 × 15 mm).…”

Section: Adherence Of Bacteria To the Surfacementioning

confidence: 99%

3D-Printed Drug Delivery Systems: The Effects of Drug Incorporation Methods on Their Release and Antibacterial Efficiency

et al. 2020

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Additive manufacturing technologies have been widely used in the medical field. More specifically, fused filament fabrication (FFF) 3D-printing technology has been thoroughly investigated to produce drug delivery systems. Recently, few researchers have explored the possibility of directly 3D printing such systems without the need for producing a filament which is usually the feedstock material for the printer. This was possible via direct feeding of a mixture consisting of the carrier polymer and the required drug. However, as this direct feeding approach shows limited homogenizing abilities, it is vital to investigate the effect of the pre-mixing step on the quality of the 3D printed products. Our study investigates the two commonly used mixing approaches—solvent casting and powder mixing. For this purpose, polycaprolactone (PCL) was used as the main polymer under investigation and gentamicin sulfate (GS) was selected as a reference. The produced systems’ efficacy was investigated for bacterial and biofilm prevention. Our data show that the solvent casting approach offers improved drug distribution within the polymeric matrix, as was observed from micro-computed topography and scanning electron microscopy visualization. Moreover, this approach shows a higher drug release rate and thus improved antibacterial efficacy. However, there were no differences among the tested approaches in terms of thermal and mechanical properties.

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“…6 P. aeruginosa is an opportunistic bacterium and the most common cause of implant-related infections due to its presence in the human microbiota, nasal cavities, and hospital surfaces, such as sinks, surgical counters, and antiseptic solutions. 7 Additionally, this bacterium is commonly associated with antibiotic resistance, 8,9 highlighting the need for advancements in surface engineering research for implants to produce surfaces more resistant to bacterial colonization through accessible and cost-effective methods.…”

Section: Introductionmentioning

confidence: 99%

Enhancing Titanium Disk Performance through In-Pack Cold Atmospheric Plasma Treatment

Martins,

da Silva Braz,

de Macedo

et al. 2024

ACS Biomater. Sci. Eng.

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While titanium dental implants have already been clinically established, ongoing research is continuously being conducted to advance the fields of osseointegration and bacterial resistance, seeking further improvements in these areas. In this study, we introduce an innovative method for treating titanium surfaces within tightly sealed packaging. Specifically, titanium discs, enclosed in surgical-grade packaging, underwent treatment using cold atmospheric plasma (CAP). The surfaces were thoroughly characterized in terms of wettability, crystalline structure, and chemical composition. Hemocompatibility analyses were conducted using blood diluted in sodium citrate (1:9) exposed to titanium discs for 30 min inside a CO 2 incubator at 37 °C. Subsequently, various blood parameters were evaluated, including prothrombin time (PT), activated partial thromboplastin time (APTT), and platelet adhesion. Microbiological analyses were also performed using Pseudomonas aeruginosa (ATCC 27853) for 4 h at 37 °C. The treatment with CAP Jet resulted in a reduction in contact angle without causing any changes in the crystalline structure. No statistically significant differences were observed in the blood parameters. The plasma-treated samples exhibited lower PT and APTT values compared to those of the control group. The surfaces treated with CAP Jet showed increased platelet activation, platelet density, and thrombus formation when compared with the untreated samples. Moreover, the treated surfaces demonstrated lower bacterial colony formation compared with other surfaces.

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Bisphosphonates offer protection against prosthetic joint infections caused by Staphylococcus aureus and Staphylococcus epidermidis biofilms

Cited by 5 publications

References 20 publications

Structural and Functional Dynamics of Staphylococcus aureus Biofilms and Biofilm Matrix Proteins on Different Clinical Materials

Structural and Functional Dynamics of Staphylococcus aureus Biofilms and Biofilm Matrix Proteins on Different Clinical Materials

3D-Printed Drug Delivery Systems: The Effects of Drug Incorporation Methods on Their Release and Antibacterial Efficiency

Enhancing Titanium Disk Performance through In-Pack Cold Atmospheric Plasma Treatment

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