Bitter melon fruit extract has a hypoglycemic effect and reduces hepatic lipid accumulation in <i>ob/ob</i> mice

Dwijayanti, Dinia Rizqi; Shimada, Takumi; Ishii, Toshinari; Okuyama, Tetsuya; Ikeya, Yukinobu; Mukai, Eri; Nishizawa, Mikio

doi:10.1002/ptr.6600

Cited by 27 publications

(17 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We have previously shown that the exposure of methanol extract of bitter melon fruit for 8 h suppresses iNOS induction and NO production in IL-1β-treated hepatocytes, and that the hydrophobic fraction is responsible for the anti-inflammatory effect (14) . In addition, we found that 7 days of administration of the hydrophobic fraction to ob/ob mice reduces hepatic lipid accumulation and blood glucose levels (15) . Increases in insulin receptor expression in hepatocytes and serum insulin levels have also been observed (15) .…”

Section: Discussionmentioning

confidence: 73%

“…In addition, we found that 7 days of administration of the hydrophobic fraction to ob/ob mice reduces hepatic lipid accumulation and blood glucose levels (15) . Increases in insulin receptor expression in hepatocytes and serum insulin levels have also been observed (15) . These observations could be attributed to the anti-inflammatory effect of the hydrophobic fraction.…”

Section: Discussionmentioning

confidence: 73%

“…We recently reported that a bitter melon fruit extract (BMFE) by methanol suppresses induction of inducible nitric oxide synthase (iNOS) and production of the proinflammatory mediator nitric oxide (NO) in interleukin (IL)-1β-treated hepatocytes from rats, and the bioactive components are included in the ethyl acetate-soluble fraction (i.e., hydrophobic fraction) of BMFE (14) . Furthermore, in our previous study, we found that cucurbitacin B, a cucurbitane-type triterpenoid, is present in the ethyl acetate-soluble fraction of BMFE and suppresses NO production in IL-1β-treated hepatocytes (15) . In addition, administration of the ethyl acetate-soluble fraction of BMFE to ob/ob mice for 7 days resulted in reduction in hepatic lipid accumulation and improvement in hyperglycemia (15) .…”

Section: Introductionmentioning

confidence: 86%

See 2 more Smart Citations

Bitter melon fruit extract enhances intracellular ATP production and insulin secretion from rat pancreatic β-cells

et al. 2021

Self Cite

View full text Add to dashboard Cite

Bitter melon (Momordica charantia L.) has been shown to have various health-promoting activities, including antidiabetic and hypoglycemic effects. Improvement in insulin sensitivity and increase in glucose utilization in peripheral tissues have been reported, but the effect on insulin secretion from pancreatic β-cells remains unclear. In this study, we investigated the effect of bitter melon fruit on insulin secretion from β-cells and the underlying mechanism. The green fruit of bitter melon was freeze-dried and extracted with methanol. The bitter melon fruit extract (BMFE) was fractionated using ethyl acetate (fraction A), n-butanol (fraction B), and water (fraction C). Insulin secretory capacity from INS-1 cells and rat pancreatic islets, as well as glucose tolerance in rats by oral glucose tolerance test (OGTT) were measured using BMFE and fractions. ATP production in β-cells was also examined. BMFE augmented insulin secretion from INS-1 cells in a dose-dependent manner. The significant augmentation of insulin secretion was independent of the glucose dose. Fraction A (i.e., hydrophobic fraction), but not fractions B and C, augmented insulin secretion significantly at the same level as that by BMFE. This finding was also observed in pancreatic islets. In OGTTs, BMFE and fraction A decreased blood glucose levels and increased serum insulin levels after glucose loading. The decrease in blood glucose levels was also observed in streptozotocin-induced diabetic rats. In addition, BMFE and fraction A increased the ATP content in β-cells. We concluded that hydrophobic components of BMFE increase ATP production and augment insulin secretion from β-cells, consequently decreasing blood glucose levels.

show abstract

Section: Discussionmentioning

confidence: 73%

Section: Discussionmentioning

confidence: 73%

Section: Introductionmentioning

confidence: 86%

See 1 more Smart Citation

Bitter melon fruit extract enhances intracellular ATP production and insulin secretion from rat pancreatic β-cells

et al. 2021

Self Cite

View full text Add to dashboard Cite

show abstract

“…Furthermore, the IL-6 and TNF-α expression in epicardial adipose tissue were also reduced [28]. Another recent study by Dwijayanti and collegues found out that the expression of interleukin-1β mRNA and hepatic lipid accumulation in hepatocytes had been reduced in ob/ob mice type 2 diabetes mellitus model after 7 days of administration with ethyl acetate soluble fraction from MC fruit [18]. In addition, a previous study that involved MC-treated rat model showed that the levels of pro-inflammatory cytokines in the liver, muscle and epididymal fats were significantly down-regulated.…”

Section: Introductionmentioning

confidence: 90%

“…(MC) or bitter gourd belongs to Cucurbitaceae family. The fruit contains bitter, cucurbitane-type triterpenoids, including charantin, cucurbitacins and momordicins [18,19]. The seeds, fruit and leaves of MC had shown a wide range of pharmacological activities, and MC has been traditionally used for its therapeutic activities including anti-diabetic, anti-inflammatory, hypocholesterolemic, hypotriglyceridemic, antitumor, hypo-tensive, immunostimulant, anti-viral, antiinflammatory, anti-oxidant, anti-leukemic, antimicrobial, anthelmintic, anti-mutagenic, anti-ulcer, and insecticidal properties [20,21,22].…”

Section: Introductionmentioning

confidence: 99%

INHIBITION OF LIPOXYGENASES AND CYCLOOXYGENASES BY Momordica charantia

et al. 2020

View full text Add to dashboard Cite

Momordica charantia (MC) is a climber belongs to the Cucurbitaceae family. While there are accumulating evidences showing its pharmacological activities, the effects of fractionation and processing on its anti-inflammatory activity have not been fully elucidated. In this study, we aimed to investigate the anti-inflammatory effects of MC raw juice and fractions from MC fruit extract and to evaluate the effect of processing methods on its anti-inflammatory activity. First, MC juice was extracted using a conventional juicer (raw juice) and fractionated using a solid phase extraction method into five fractions; strong acid, weak acid, neutral, weak base, and strong base fractions. The raw juice and its fractions then were tested for their anti-inflammatory activities including cyclooxygenase-1 (COX-1), cyclooxygenase-2 (COX-2), 5-lipoxygenase (5-LOX), and 15-lipoxygenase (15-LOX) inhibition assays. The results showed that the COX-1 inhibition activity of MC can be attributed to the neutral metabolites (52.60% inhibition). In contrast, the anti-COX-2 activity of MC was found exerted collectively by metabolites in multiple fractions (strong base, neutral, strong acid fractions). Contrarily, metabolites from all five fractions contributed to the MC inhibition activities on 5-LOX (55.51% inhibition for raw juice) and 15-LOX (55.3% inhibition for raw juice). Additionally, the current findings showed that the juice processing technique influenced the plant bioactivity, where juice extract obtained from slow juicer showed consistently higher anti-inflammatory activities than juice from conventional juicer. In summary, the current study provided evidences of anti-inflammatory action of MC and its fractions, which may contribute towards an improved processing and fractionation strategy.

show abstract

Protecting‐Group‐Free One‐Step Palladium‐Catalyzed Coupling on C25 of Cucurbitacin B Expands Chemical Diversity with Improved Cytotoxicity against A549 Cells

Zhuo

Cao

et al. 2022

Chin. J. Chem.

View full text Add to dashboard Cite

Comprehensive Summary The natural product cucurbitacin B has been widely studied because of its multiple biological activities, especially its potent antitumor effects. However, modifications of cucurbitacin B are mainly focused on the C2 and C16 site, studies on the C25 acetoxy group are still limited. We successfully developed a palladium‐catalyzed allylic coupling of cucurbitacin B with boronic acids, providing a one‐step approach to expand the chemical diversity of the C25 position. Our method was protecting‐group‐free, showing a good functional group tolerance and a wide substrate scope under mild reaction conditions. A library of 29 derivatives was prepared, compounds 2q and 2u showed higher cytotoxicity against A549 cells than cucurbitacin B, compounds 2n and 2o maintained potency, and the introduced hydroxyl and amino groups could be further derived.

show abstract

Bitter melon fruit extract has a hypoglycemic effect and reduces hepatic lipid accumulation in ob/ob mice

Cited by 27 publications

References 28 publications

Bitter melon fruit extract enhances intracellular ATP production and insulin secretion from rat pancreatic β-cells

Bitter melon fruit extract enhances intracellular ATP production and insulin secretion from rat pancreatic β-cells

INHIBITION OF LIPOXYGENASES AND CYCLOOXYGENASES BY Momordica charantia

Protecting‐Group‐Free One‐Step Palladium‐Catalyzed Coupling on C25 of Cucurbitacin B Expands Chemical Diversity with Improved Cytotoxicity against A549 Cells

Contact Info

Product

Resources

About