Bivalve transcriptomics reveal pathogen sequences and a powerful immune response of the Mediterranean mussel (Mytilus galloprovincialis)

Moreira, Rebeca; Balseiro, Pablo; Forn-Cuní, Gabriel; Milan, Massimo; Bargelloni, Luca; Novoa, Beatriz; Figueras, António

doi:10.1007/s00227-018-3308-0

Cited by 20 publications

(14 citation statements)

References 107 publications

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“…This function has been indeed demonstrated in the immune response of Mytilus galloprovincialis ( Estévez-Calvar et al. 2011 ; Moreira et al. 2018 ).…”

Section: Discussionmentioning

confidence: 69%

Piercing Fishes: Porin Expansion and Adaptation to Hematophagy in the Vampire Snail Cumia reticulata

Gerdol

Cervelli

Oliverio

et al. 2018

Molecular Biology and Evolution

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Cytolytic pore-forming proteins are widespread in living organisms, being mostly involved in both sides of the host–pathogen interaction, either contributing to the innate defense or promoting infection. In venomous organisms, such as spiders, insects, scorpions, and sea anemones, pore-forming proteins are often secreted as key components of the venom. Coluporins are pore-forming proteins recently discovered in the Mediterranean hematophagous snail Cumia reticulata (Colubrariidae), highly expressed in the salivary glands that discharge their secretion at close contact with the host. To understand their putative functional role, we investigated coluporins’ molecular diversity and evolutionary patterns. Coluporins is a well-diversified family including at least 30 proteins, with an overall low sequence similarity but sharing a remarkably conserved actinoporin-like predicted structure. Tracking the evolutionary history of the molluscan porin genes revealed a scattered distribution of this family, which is present in some other lineages of predatory gastropods, including venomous conoidean snails. Comparative transcriptomic analyses highlighted the expansion of porin genes as a lineage-specific feature of colubrariids. Coluporins seem to have evolved from a single ancestral porin gene present in the latest common ancestor of all Caenogastropoda, undergoing massive expansion and diversification in this colubrariid lineage through repeated gene duplication events paired with widespread episodic positive selection. As for other parasites, these findings are congruent with a “one-sided arms race,” equipping the parasite with multiple variants in order to broaden its host spectrum. Overall, our results pinpoint a crucial adaptive role for coluporins in the evolution of the peculiar trophic ecology of vampire snails.

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“…This function has been indeed demonstrated in the immune response of Mytilus galloprovincialis ( Estévez-Calvar et al. 2011 ; Moreira et al. 2018 ).…”

Section: Discussionmentioning

confidence: 69%

Piercing Fishes: Porin Expansion and Adaptation to Hematophagy in the Vampire Snail Cumia reticulata

Gerdol

Cervelli

Oliverio

et al. 2018

Molecular Biology and Evolution

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“…Most of the transcriptomes assembled in marine bivalves did not report fragmentation percentage in their results; however, fragmentation is known to be typical in de novo transcriptome assemblies [55]. The assembly statistics in this study was according to most published marine bivalve transcriptomes referred by the authors as high quality assembly [56,57,58] and showed a higher quality assembly compared to others [27,59,60].…”

Section: Discussionmentioning

confidence: 75%

“…Particularly, these analyses have provided information to analyse complex relations among disease, drugs, and organisms [22]. Moreover, RNA-seq has been used in marine bivalves to understand physiological stress [23,24], toxicological effects [25,26], and resistance to disease and immunology [27,28]. In this sense, the RNA-seq analysis helps to understand the molecular response activated by the effect of time-dependent diluted immunomodulatory treatments, which allowed identifying the down-and up-regulated genes of juvenile scallops in response to HDIC.…”

Section: Introductionmentioning

confidence: 99%

Transcriptome analysis of Catarina scallop (Argopecten ventricosus) juveniles treated with highly-diluted immunomodulatory compounds reveals activation of non-self-recognition system

et al. 2020

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Marine bivalve hatchery productivity is continuously challenged by apparition and propagation of new diseases, mainly those related to vibriosis. Disinfectants and antibiotics are frequently overused to prevent pathogen presence, generating a potential negative impact on the environment. Recently, the use of highly diluted compounds with immunostimulant properties in marine organisms has been trailed successfully to activate the self-protection mechanisms of marine bivalves. Despite their potential as immunostimulants, little is known about their way of action. To understand their effect, a comparative transcriptomic analysis was performed with Argopecten ventricosus juveniles. The experimental design consisted of four treatments formulated from pathogenic Vibrio lysates at two dilutions: [(T1) Vibrio parahaemolyticus and Vibrio alginolyticus 1D; (T2) V. parahaemolyticus and V. alginolyticus 7C]; minerals [(T3) PhA+SiT 7C], scorpion venom [(T4) ViT 31C]; and one control (C1) hydro-alcoholic solution (ethanol 1%). The RNA sequencing (RNAseq) analysis showed a higher modulation of differentially expressed genes (DEG) in mantle tissue compared to gill tissue. The scallops that showed a higher number of DEG related to immune response in mantle tissue corresponded to T1 (V. parahaemolyticus and V. alginolyticus lysate) and T3 (Silicea terra®-Phosphoric acid®). The transcriptome analysis allowed understanding some interactions between A. ventricosus juveniles and highly-diluted treatments.

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“…To understand the immune reaction of these animals, a considerable effort has been made in recent years to increase the genomic and transcriptomic resources in bivalves and specifically in the Mediterranean mussel, M . galloprovincialis 15–20 .…”

Section: Introductionmentioning

confidence: 99%

High individual variability in the transcriptomic response of Mediterranean mussels to Vibrio reveals the involvement of myticins in tissue injury

Rey-Campos

Moreira

Valenzuela-Muñoz

et al. 2019

Sci Rep

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Mediterranean mussels ( Mytilus galloprovincialis ) are sessile filter feeders that live in close contact with numerous marine microorganisms. As all invertebrates, they lack an adaptive immune response and how these animals are able to respond to a bacterial infection and discriminate it from their normal microbiome is difficult to understand. In this work, we conducted Illumina sequencing of the transcriptome of individual mussels before and after being infected with Vibrio splendidus . The control mussels were injected with filtered seawater. We demonstrate that a great variability exists among individual transcriptomes and that each animal showed an exclusive repertoire of genes not shared with other individuals. The regulated genes in both the control and infected mussels were also analyzed and, unexpectedly, the sampling before the injection was considered a stress stimulus strong enough to trigger and modulate the response in hemocytes, promoting cell migration and proliferation. We found a clear response against the injection of filtered seawater, suggesting a reaction against a tissue injury in which the myticins, the most expressed antimicrobial peptides in mussel, appeared significantly up regulated. Functional experiments with flow cytometry confirmed the transcriptomic results since a significant alteration of hemocyte structures and a decrease in the number of hemocytes positive for myticin C were found only after a Vibrio infection and not observed when mussels were bled before, generating a tissue injury. Therefore, we report the involvement of myticins in the response to a danger signal such as a simple injection in the adductor muscle.

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Bivalve transcriptomics reveal pathogen sequences and a powerful immune response of the Mediterranean mussel (Mytilus galloprovincialis)

Cited by 20 publications

References 107 publications

Piercing Fishes: Porin Expansion and Adaptation to Hematophagy in the Vampire Snail Cumia reticulata

Piercing Fishes: Porin Expansion and Adaptation to Hematophagy in the Vampire Snail Cumia reticulata

Transcriptome analysis of Catarina scallop (Argopecten ventricosus) juveniles treated with highly-diluted immunomodulatory compounds reveals activation of non-self-recognition system

High individual variability in the transcriptomic response of Mediterranean mussels to Vibrio reveals the involvement of myticins in tissue injury

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