BjuWRR1, a CC-NB-LRR gene identified in Brassica juncea, confers resistance to white rust caused by Albugo candida

Arora, Heena; Padmaja, K Lakshmi; Paritosh, Kumar; Mukhi, Nitika; Tewari, A. K.; Mukhopadhyay, Arnab; Gupta, Vibha; Pradhan, Akshay K.; Pental, Deepak

doi:10.1007/s00122-019-03350-z

Cited by 56 publications

(20 citation statements)

References 63 publications

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“…Phylogenetic analysis carried out as described in the materials and methods section revealed the presence of four subgroups-CNL-A, CNL-B, CNL-C and CNL-D (Supplementary Figure S4) as reported earlier (Meyers et al, 2003). The candidate gene, BjuA046215, was found to be present in the CNL-D subgroup of the phylogenetic tree along with BjuWRR1, a CNL type R gene identified in B. juncea Donskaja-IV on LG A5 (Arora et al, 2019) (Figure 5).…”

Section: Evolutionary Relationship Of Bjua046215supporting

confidence: 71%

“…We assessed sequence similarity/diversity and relationship of the candidate gene BjuA046215 with the well-characterized white rust resistance-conferring gene BjuWRR1 (Arora et al, 2019) and the genes present in A. thaliana and B. rapa belonging to the CNL clade (Yu et al, 2014). Phylogenetic analysis carried out as described in the materials and methods section revealed the presence of four subgroups-CNL-A, CNL-B, CNL-C and CNL-D (Supplementary Figure S4) as reported earlier (Meyers et al, 2003).…”

Section: Evolutionary Relationship Of Bjua046215mentioning

confidence: 77%

“…BjuA046215 has a structure that is typical of CC-NBS-LRR class of R type genes and an inheritance pattern of a single dominant gene usually encountered in effectortriggered immunity (ETI) based on R-Avr interaction (Jones and Dangl, 2006;Cevik et al, 2019). BjuA046215 is phylogenetically related to the CNL type R gene BjuWRR1, identified in the east European gene pool line Donskaja-IV that has been shown to confer resistance to a number of white rust isolates including AcB1 (Arora et al, 2019). BjuA046215 can, therefore, be named as BjuA6.WRR.b1 (in short BjuWRR2) as it is the second R gene found to confer resistance to A. candida in B. juncea.…”

Section: Discussionmentioning

confidence: 99%

“…The Indian gene pool lines of Brassica juncea (oilseed mustard) are highly susceptible to A. candida (Li et al, 2008;Panjabi-Massand et al, 2010;Awasthi et al, 2012) leading to significant yield losses (Lakra and Saharan, 1989;Saharan and Verma, 1992). However, resistance to white rust has been reported in the east European gene pool lines of oleiferous mustard (Panjabi-Massand et al, 2010;Awasthi et al, 2012;Arora et al, 2019). Breeding for resistance to white rust in mustard is of critical importance as in India alone the crop is grown in~6 million hectares of land.…”

Section: Introductionmentioning

confidence: 99%

“…The two loci have been introgressed into four major varieties grown extensively in India. The gene conferring resistance to A. candida in the locus AcB1-A5.1 was identified to be a CC-NBS-LRR (CNL) type R gene named as BjuA5.WRR.a1 (in short-BjuWRR1) that conferred resistance to several isolates of A. candida collected from different locations in the mustard growing regions of India (Arora et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

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A Mapped Locus on LG A6 of Brassica juncea Line Tumida Conferring Resistance to White Rust Contains a CNL Type R Gene

et al. 2020

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White rust, causal agent oomycete Albugo candida, is a significant disease of the cultivated Brassica species. The Indian gene pool lines of oilseed mustard, Brassica juncea, are highly susceptible to the pathogen. Resistance to A. candida has been reported in the east European gene pool lines of mustard and mapped to LG A4 in line Heera and LG A5 in line Donskaja-IV. A new resistance-conferring locus to A. candida isolate AcB1 has been mapped to LG A6 of B. juncea line Tumida-a Chinese vegetable type mustard using an F 1 DH mapping population that has been developed from a Tumida × Varuna (susceptible Indian gene pool line) cross. A molecular map containing 8,303 genic and GBS markers was used to map the resistance trait to an interval of 63.0 cM-70.8 cM on LG A6. Genome assemblies of Tumida and Varuna were used to find the genes present within the flanking markers discerned by genetic mapping. The most likely candidate gene in the mapped interval is BjuA046215, a CC-NBS-LRR (CNL) type R gene that encodes a protein with all the specific subdomains of the proteins encoded by such genes. Alleles of BjuA046215 in Varuna and other lines of the Indian and the east European gene pools encode proteins that have truncated LRR domains. Analysis of the syntenic regions in some of the Brassicaceae genomes and phylogenetic analysis of CNL type R genes showed BjuA046215 to be closely related to a recently described white rust resistance-conferring R gene BjuWRR1 in B. juncea Donskaja-IV, both belonging to the CNL-D group of R genes. Related R genes in Arabidopsis thaliana confer resistance to another oomycete, Peronospora parasitica.

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Section: Evolutionary Relationship Of Bjua046215supporting

confidence: 71%

Section: Evolutionary Relationship Of Bjua046215mentioning

confidence: 77%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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A Mapped Locus on LG A6 of Brassica juncea Line Tumida Conferring Resistance to White Rust Contains a CNL Type R Gene

et al. 2020

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Comparison and evolutionary analysis of Brassica nucleotide binding site leucine rich repeat (NLR) genes and importance for disease resistance breeding

2020

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The Brassica genus contains many agriculturally significant oilseed and vegetable crops, however the crop yield is threatened by a range of fungal and bacterial pathogens. Nucleotide Binding Site Leucine Rich Repeat (NLR) genes play important roles in plant innate immunity. The evolution of NLR genes is influenced by genomic processes and pathogen selection. At the whole genome level, whole genome duplications (WGDs) generate abundant gene copies, most of which are lost during genome fractionation. At sub‐genomic levels, some retained copies undergo duplication forming clusters which facilitate rapid evolution through recombination. The number, distribution and genetic variations of the NLR genes vary among Brassica species and within populations suggesting differential selection pressure exerted by pathogen populations throughout the evolutionary history. A study of the evolution of disease resistance genes in agriculturally important plants such as Brassicas helps gain insights into their function and inform the identification of resistance genes for breeding of resistant lines.

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Genetics and Genomic Approaches for Disease Resistance in Brassicas

Priyamedha

Ram

Kumar

et al. 2020

Brassica Improvement

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BjuWRR1, a CC-NB-LRR gene identified in Brassica juncea, confers resistance to white rust caused by Albugo candida

Cited by 56 publications

References 63 publications

A Mapped Locus on LG A6 of Brassica juncea Line Tumida Conferring Resistance to White Rust Contains a CNL Type R Gene

A Mapped Locus on LG A6 of Brassica juncea Line Tumida Conferring Resistance to White Rust Contains a CNL Type R Gene

Comparison and evolutionary analysis of Brassica nucleotide binding site leucine rich repeat (NLR) genes and importance for disease resistance breeding

Genetics and Genomic Approaches for Disease Resistance in Brassicas

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