BK and JC virus infections in healthy patients compared to kidney transplant recipients in Tunisia

Boukoum, Hanen; Nahdi, Imen; Sahtout, W.; Skiri, Habib; Segondy, Michel; Aouni, Mahjoub

doi:10.1016/j.micpath.2016.06.015

Cited by 25 publications

(21 citation statements)

References 33 publications

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“…After primary infection, which is usually asymptomatic, JCPyV remains lifelong in the host in a latent phase (Kean, Rao, Wang, & Garcea, ; Knowles, ; Stolt, Sasnauskas, Koskela, Lehtinen, & Dillner, ). Reactivation may occur in normal subjects and immunodepressed/suppressed patients (Boukoum et al, ; Delbue et al, ; Rodio et al, ).…”

Section: Introductionmentioning

confidence: 99%

Serum IgG antibodies from healthy subjects up to 100 years old react to JC polyomavirus

Bononi

Mazzoni

Pietrobon

et al. 2018

Journal Cellular Physiology

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JC polyomavirus (JCPyV) was identified in 1971 in the brain tissue of a patient (J.C.) affected by the progressive multifocal leukoencephalopathy (PML). JCPyV encodes for the oncoproteins large T antigen (Tag) and small t-antigen (tag). These oncoproteins are responsible of the cell transformation and tumorigenesis in experimental animals. JCPyV is ubiquitous in human populations. After the primary infection, which is usually asymptomatic, JCPyV remains lifelong in the host in a latent phase. Its reactivation may occur in heathy subjects and immunocompromised patients. Upon reactivation, JCPyV could reach (i) the CNS inducing the PML, (ii) the kidney of transplant patients causing the organ rejection. Association between JCPyV, which is a small DNA tumor virus, and gliomas and colorectal carcinomas has been published. In the present investigation, we report on a new indirect ELISA with two specific synthetic peptides mimicking JCPyV VP1 immunogenic epitopes to detect specific serum IgG antibodies against JCPyV. Serum samples of healthy subjects (n = 355) ranging 2-100 years old, were analyzed by this new indirect ELISA. The linear peptides VP1 K and VP1 N resemble the natural JCPyV VP1 capsidic epitopes constituting a docking site for serum antibodies. Data from this innovative immunologic assay indicate that the overall prevalence of JCPyV-VP1 antibodies in healthy subjects is at 39%. The innovative indirect ELISA with JCPyV VP1 mimotopes seems to be a useful method to detect specific IgG antibodies against this virus, without cross-reactivity with the closely related SV40 and BKPyV polyomaviruses.

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Section: Introductionmentioning

confidence: 99%

Serum IgG antibodies from healthy subjects up to 100 years old react to JC polyomavirus

Bononi

Mazzoni

Pietrobon

et al. 2018

Journal Cellular Physiology

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“…Other studies of transplant patients and normal population have observed a JCV prevalence between 9.5-38% [12,13,14]. Indeed, Boukoum et al [13] detected JCV viruria in 13% of all healthy individuals, but in 38% of all kidney transplant recipients in their test population. In Saundh et al [14], JCV was indicated in 15 of 112 cases (13.3%).…”

Section: Discussionmentioning

confidence: 89%

Frequency of JC Virus Appearance in the Urine of Post-Transplantation Patients

Boguszewska

Woś

Jarzynski

et al. 2018

Current Issues in Pharmacy and Medical Sciences

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Polyomaviruses are common viruses that induce various infections in many mammalian species, including humans. The best-known viruses of this kind are BKV and JCV. The aim of the study was to analyze the incidence of infection caused by JCV in a group of patients after kidney or bone marrow transplant, and to analyze JCV genetic diversity in post-transplantation recipients. The study group included 81 patients after kidney transplantation treated in the Independent Public Teaching Hospital No 4 in Lublin and a group of 24 patients after marrow transplantation from the Children's Clinical Hospital of Lublin. The research material included 105 DNA probes from urine samples that were tested via the PCR method for the presence of JCV genetic material. Amplification products were separated in agarose gel, positive PCR products were subjected to purification and the pure product was sent to sequencing. Pearson's chi-square test was used to investigate the relationship between the prevalence of JCV viruria and study group and gender. Statistical significance was defined as p < 0.05. A JCV positive result was discovered in 27.6% of all samples. In the group of adults, 34.6% were positive, while in children, this was 3.4%. Among all patients, only 8 women were infected with the JC virus, while 21 men were. We saw that the incidence of infection caused by the JC virus increases with age. Moreover, JCV DNA is more frequently isolated from men than from women. JCV infections are also a more common cause of infections in patients after renal transplantation than in bone marrow transplant patients

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“…Even immunocompetent patients excreted BKV and JCV in the urine, showing that the final diagnosis always depends on a combination of laboratory and clinical data. Boukoum et al reported that in contrast to transplant recipients in whom BKV was more frequent, JCV was more commonly observed in healthy subjects. The authors emphasize that the 2 Polyomaviruse s had different associations with age and gender according to the population.…”

Section: Discussionmentioning

confidence: 99%

JC and BK virus DNA detection in archival slides of urine cytospin from renal transplant patients

Assis

Carvalho

Silva

et al. 2018

Transplant Infectious Dis

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BK and JC virus infections in healthy patients compared to kidney transplant recipients in Tunisia

Cited by 25 publications

References 33 publications

Serum IgG antibodies from healthy subjects up to 100 years old react to JC polyomavirus

Serum IgG antibodies from healthy subjects up to 100 years old react to JC polyomavirus

Frequency of JC Virus Appearance in the Urine of Post-Transplantation Patients

JC and BK virus DNA detection in archival slides of urine cytospin from renal transplant patients

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