BK polyomavirus association with colorectal cancer development

Khabaz, Mohamad Nidal; Nedjadi, Taoufik; Gari, Mamdooh; Atta, Hazem; Basuni, Ashraf A.; El-Derwi, Douaa

doi:10.4238/gmr.15027841

Cited by 6 publications

(6 citation statements)

References 29 publications

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“…For BKPyV, our results are in line with the data in literature, that did not report evidence of BKPyV involvement in colon cancer pathogenesis 29,45–48 . Only two studies detected BKPyV genome and proteins in colon cancer tissues 15,16…”

Section: Discussionsupporting

confidence: 91%

“…For BKPyV, our results are in line with the data in literature, that did not report evidence of BKPyV involvement in colon cancer pathogenesis. 29,[45][46][47][48] Only two studies detected BKPyV genome and proteins in colon cancer tissues. 15,16 JCPyV has been so far the most studied HPyVs for its possible association with colon cancer pathogenesis, as documented in several studies, where the genome of JCPyV was amplified in the cells isolated from the gastro-intestinal tract, both in pre-neoplastic lesions and in malignant cells.…”

Section: Discussionmentioning

confidence: 99%

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Human polyomaviruses genomes in clinical specimens of colon cancer patients

Dolci

Signorini

Toumi

et al. 2021

Journal of Medical Virology

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Colon cancer is the third cause of cancer death in the developed countries. Some environmental factors are involved in its pathogenesis, including viral infections. The possible involvement of human polyomaviruses (HPyVs) in colon cancer pathogenesis has been previously reported, leading to inconsistent conclusions. Clinical specimens were collected from 125 colon cancer patients. Specifically, 110 tumor tissues, 55 negative surgical margins, and 39 peripheral blood samples were analyzed for the presence of six HPyVs: JC polyomavirus (JCPyV), BK polyomavirus (BKPyV), Merkel cell PyV (MCPyV), HPyV −6, −7, and −9 by means of DNA isolation and subsequent duplex Real Time quantitative polymerase chain reaction. HPyVs genome was detected in 33/204 samples (16.2%): the significant higher positivity was found in tumor tissues (26/110, 23.6%), followed by negative surgical margins (3/55, 5.5%, p < .05), and peripheral blood mononuclear cells (PBMCs) (4/39; 10.3%).HPyVs load was statistically higher only in the tumor tissues compared to negative surgical margins (p < .05). Specifically, MCPyV was detected in 19.1% (21/110) of tumor tissues, 3.6% (2/55) of negative surgical margins (p < .05), and 7.7% (3/39) of PBMCs; HPyV-6 in 2.7% (3/110) of tumor tissues, and 1.8% (1/55) of negative surgical margins; one tumor tissue (1/110, 0.9%) and one PBMCs sample (1/39, 2.6%) were positive for BKPyV; JCPyV was present in 0.9% (1/110) of tumor tissues.HPyV-7 and 9 were not detected in any sample. High prevalence and load of MCPyV genome in the tumor tissues might be indicative of a relevant rather than bystander role of the virus in the colon tumorigenesis.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 99%

Human polyomaviruses genomes in clinical specimens of colon cancer patients

Dolci

Signorini

Toumi

et al. 2021

Journal of Medical Virology

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“…BKPyV has been detected in cancerous tissues from the bladder, prostate, pancreas, adrenal glands, and brain [ 23 – 26 ]. In contrast, in a number of research studies, BKPyV has not been found in comparable tumors [ 27 – 29 ].…”

Section: Introductionmentioning

confidence: 92%

Case-control study: Unveiling human polyomaviruses and papillomavirus in Egyptian colorectal cancer patients

Darwish,

Allayeh,

Ahmed

et al. 2024

PLoS ONE

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Background Colorectal cancer (CRC) is a cancer type that is thought to be influenced by human papillomaviruses (HPVs) and human polyomaviruses (HPyVs). In Egypt, CRC ranks as the 7th most common cancer, accounting for 3.47% of male cancers and 3% of female cancers. However, there is currently a lack of information regarding the presence of PyVs and HPVs co-infection specifically in CRC cases in Egypt. Therefore, the aim of this study was to investigate the occurrence of HPVs and HPyVs (JCPyV, BKPyV, and SV40) infections, as well as co-infections, among CRC patients in Egypt. Additionally, the study aimed to assess any potential association between these viral infections and tumor stages. Methods In the present study, we analyzed a total of 51 tissue samples obtained from Egyptian CRC patients, along with 19 polyps’ samples. Our investigation focused on the detection and genotyping of HPyVs using Real-Time PCR. Additionally, we employed real-time PCR for the detection of HPVs, and for their genotyping, we utilized a combination of PCR amplification followed by sequencing. Results In our study, we found evidence of HPyVs infection in the CRC patients, specifically SV40 (25.5%) and BKPyV (19.6%). However, JCPyV was not detected in the samples that were examined. Additionally, we discovered that HPV was present in 43.1% of the CRC patients. When considering viral co-infections, 19.6% of the CRC samples showed coexistence of multiple viruses, while no co-infections were found in the polyps samples. Importantly, we observed a significant correlation between the presence of HPVs and advanced colorectal tumor grades B2 and D. Conclusion Our findings provide valuable data for the detection of oncogenic viruses in colorectal cancer (CRC) and underscore the association of viral co-infections with advanced tumor stages. However, further research with larger cohorts is necessary to validate these findings and strengthen their significance in the field of CRC.

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“…Polyomaviruses encode the T-antigen, a non-structural oncogenic protein that is capable of inactivating tumor suppressor proteins p53 and pRB, among other mechanisms of signaling pathway interference [36]. Specific oncogenic mechanisms remain unknown for human polyomaviruses in colorectal cancer, but are implied by their presence in cancerous colorectal tissue samples [37].…”

Section: How Can the Virome Induce Carcinogenesis?mentioning

confidence: 99%

Enteric Virome and Carcinogenesis in the Gut

Emlet

Lamendella

2020

Dig Dis Sci

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Colorectal cancer (CRC) is a leading cause of cancer-related deaths in both the USA and the world. Recent research has demonstrated the involvement of the gut microbiota in CRC development and progression. Microbial biomarkers of disease have focused primarily on the bacterial component of the microbiome; however, the viral portion of the microbiome, consisting of both bacteriophages and eukaryotic viruses, together known as the virome, has been lesser studied. Here we review the recent advancements in high-throughput sequencing (HTS) technologies and bioinformatics, which have enabled scientists to better understand how viruses might influence the development of colorectal cancer. We discuss the contemporary findings revealing modulations in the virome and their correlation with CRC development and progression. While a variety of challenges still face viral HTS detection in clinical specimens, we consider herein numerous next steps for future basic and clinical research. Clinicians need to move away from a single infectious agent model for disease etiology by grasping new, more encompassing etiological paradigms, in which communities of various microbial components interact with each other and the host. The reporting and indexing of patient health information, socioeconomic data, and other relevant metadata will enable identification of predictive variables and covariates of viral presence and CRC development. Altogether, the virome has a more profound role in carcinogenesis and cancer progression than once thought, and viruses, specific for either human cells or bacteria, are clinically relevant in understanding CRC pathology, patient prognosis, and treatment development.

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BK polyomavirus association with colorectal cancer development

Cited by 6 publications

References 29 publications

Human polyomaviruses genomes in clinical specimens of colon cancer patients

Human polyomaviruses genomes in clinical specimens of colon cancer patients

Case-control study: Unveiling human polyomaviruses and papillomavirus in Egyptian colorectal cancer patients

Enteric Virome and Carcinogenesis in the Gut

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