BK virus and a new type of JC virus excreted by HIV-1 positive patients in rural Tanzania

Ht, Agostini; Gr, Brubaker; Shao, John F.; Levin, A.; Cf, Ryschkewitsch; Wa, Blattner; Gl, Stoner

doi:10.1007/bf01322682

Cited by 73 publications

(64 citation statements)

References 40 publications

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“…These were two Type 3 strains, one Type 1 and two Type 6 strains. One of the Type 3 strains (L1059) showed identical sequence in the VP1 fragment to the DNA sequence of strain #309 previously amplified from the urine of an African from Mara region in Tanzania (Agostini et al 1995). The other Type 3 strain (L1066) showed partial sequence homology with #311(Type 3B), previously sequenced from an African-American, but differed from this strain at position 1870 where deoxyadenosine was inserted in place of deoxyguanosine.…”

Section: Resultsmentioning

confidence: 76%

“…This region is the site of a mutation changing a glutamine codon to leucine at amino acid 301. This point mutation is characteristic of all African and some Asian strains of JCV so far studied (Agostini et al 1995(Agostini et al , 1997a) (JTP-5 nucleotides, 3621-3642, 5'-CTTTGTTTGGCTGCTA CAGTAT-3' and JTP-6 nucleotides, 3896-3877, 5'-GCCTTAAGGAGC ATGACTTT-3'). The non coding regulatory regions and T-antigen intron were amplified using the primer pairs JRR-25 & 28 and JSP-1 & 2 respectively.…”

Section: Methodsmentioning

confidence: 95%

“…JCV genotypes were identified as previously described (Ault & Stoner 1992, Agostini et al 1995, 1997b, 1997e, 1998. Sequence relationships were analyzed with GCG programs, Unix version 8 (Genetics Computer Group, Madison, WI).…”

Section: Methodsmentioning

confidence: 99%

“…Based on sequence analysis of JCV complete genomes, as well as segments of the VP1 and T antigen genes, JCV can be classified into several geographically based genotypes and subtypes (Ault & Stoner 1992, Agostini et al 1995, 1997d, Sugimoto et al 1997. The major genotypes so far described are Type 1, which is of European origin, Type 2, which is Asian, and Types 3 and 6 which are African in origin (Agostini et al 1995(Agostini et al , 1998.…”

mentioning

confidence: 99%

“…The major genotypes so far described are Type 1, which is of European origin, Type 2, which is Asian, and Types 3 and 6 which are African in origin (Agostini et al 1995(Agostini et al , 1998.…”

mentioning

confidence: 99%

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Molecular Epidemiology of Human Polyomavirus JC in the Biaka Pygmies and Bantu of Central Africa

Chima

Ryschkewitsch²,

Stoner³

1998

Mem. Inst. Oswaldo Cruz

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Section: Resultsmentioning

confidence: 76%

Section: Methodsmentioning

confidence: 95%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Molecular Epidemiology of Human Polyomavirus JC in the Biaka Pygmies and Bantu of Central Africa

Chima

Ryschkewitsch²,

Stoner³

1998

Mem. Inst. Oswaldo Cruz

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Detection of archetype and rearranged variants of JC virus in multiple tissues from a pediatric PML patient

Newman

Frisque

1997

J. Med. Virol.

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JC virus (JCV) establishes persistent infections in its human host, and in some immunocompromised individuals, the virus causes the fatal brain disease progressive multifocal leukoencephalopathy (PML). Two forms of the virus, archetype and rearranged, have been isolated, with the latter being derived from the archetype form by deletion and duplication of sequences within the viral transcriptional control region (TCR). We have used the PCR technique to amplify JCV TCR sequences present within multiple tissues of a pediatric PML patient and have cloned and sequenced the PCR products. Archetype JCV was readily detected in kidney tissue; this form of JCV was also identified for the first time in brain and lymph node tissue by employing archetype-specific PCR primers. In addition, several archetype-like variants containing small deletions within their regulatory regions were isolated from cardiac muscle and lung. Different, but related rearranged forms were detected in most of the tissue examined. Each of the rearranged TCRs lacked portions of a 66 base pair (bp) region found within the archetype promoter-enhancer but retained a 23 bp region that is deleted in the prototype (Mad 1) rearranged form of JCV. Although several rearranged forms of JCV were identified in this patient, the TCRs could be assigned to one of two groups based upon the deletion boundaries generated during the adaptation from archetype to rearranged JCV. This study is the first to characterize multiple JCV variants present in different tissues from a patient likely to have succumbed to PML during a primary infection.

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Identification of JC virus variants in multiple tissues of pediatric and adult PML patients

Newman

Frisque

1999

J. Med. Virol.

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The transcriptional control region (TCR) of JC virus (JCV), the causative agent of progressive multifocal leukoencephalopathy (PML), undergoes rearrangement during replication of the virus in its human host. The mechanism by which viral promoter/enhancer sequences are deleted and duplicated within the TCR of the archetype form of JCV is not understood, but it is hypothesized that the generation of JCV variants with rearranged TCRs contributes to the virus's pathogenic potential. In a recent study of a pediatric PML patient, we detected extensive rearrangement of the JCV TCR in multiple tissues, and the archetype TCR was amplified from sites other than the kidney. These findings differed from those of previous studies that had examined tissues from adult PML patients. Since exposure to JCV usually occurs early in life, it is likely that some pediatric cases of PML arise as the result of a primary infection, whereas adult cases of PML are thought to result from the reactivation of an infection suffered as an immunocompetent child. To investigate further whether rearrangement of the JCV TCR is affected by the host's age and immune status at the time of exposure, a second pediatric patient and two adult PML patients were examined. As in our first study, multiple tissues were found to contain JCV DNA; however, fewer rearranged variants were detected. In one adult patient, related rearranged variants were detected in the brain, while archetype JCV was found in the other tissues. Based on differences in their VP1 sequences, these two forms represented different JCV genotypes, indicating that this patient had suffered a dual infection. The relevance of these findings to the rearrangement process that alters the JCV TCR is discussed.

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BK virus and a new type of JC virus excreted by HIV-1 positive patients in rural Tanzania

Cited by 73 publications

References 40 publications

Molecular Epidemiology of Human Polyomavirus JC in the Biaka Pygmies and Bantu of Central Africa

Molecular Epidemiology of Human Polyomavirus JC in the Biaka Pygmies and Bantu of Central Africa

Detection of archetype and rearranged variants of JC virus in multiple tissues from a pediatric PML patient

Identification of JC virus variants in multiple tissues of pediatric and adult PML patients

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