“…DNA was isolated using DNeasy Plant Mini Kit of the Quiagen. We studied nine polymorphic and unliked SSR: CsCAT 3, CsCAT 16, CsCAT 14, and CsCAT 41 (Marinoni, Akkak, Bounous, Edwards, & Botta, 2003), EMCs 2, EmCs 14, and EMCs 15 (Buck, Hadonou, James, Blakesley, & Russel, 2003) developed for Castanea sativa and QpZAG 36 and QpZAG 110 (Kampfer, Lexer, Glo¨ssl, & Steinkellner, 1998;Steinkellner et al, 1997) developed for Quercus petrae. DNA extraction and amplification of microsatellite loci were done following the same methodology used by Pereira-Lorenzo et al (2010).…”