Untitled

Tan, Elizabeth L; Shah, Hemanshu S.; Leister, Kirk J.; Kozick, Linda M.; Pasciak, Pamela; Vanderlaan, Roger K.; Yu, Cheng-Der; Patel, Bhiku

doi:10.1023/a:1018940907187

Cited by 9 publications

(2 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The antibacterial susceptibility test was adapted from Tan et al [54]. A broth solution containing 2% of the chitosan/small area GO (CS/3sGO) solution was prepared, where it was inoculated with the bacterial inoculum.…”

Section: Antibacterial Susceptibility Testingmentioning

confidence: 99%

Facile preparation of graphene-based chitosan films: Enhanced thermal, mechanical and antibacterial properties

Lim

Huang

Loo

2012

Journal of Non-Crystalline Solids

139

View full text Add to dashboard Cite

Chitosan is considered a model polymer because of its excellent biocompatibility, biodegradability, antibacterial property and metal binding ability. Despite the favorable properties, the poor mechanical strength and the loss of structural integrity limits the applications of chitosan. Graphene's intrinsic low weight with excellent thermal stability makes it an ideal filler for reinforcing polymers. In this work, we prepared graphene oxide (GO) via Hummer's method and simplified Hummer's method using graphite powder and graphite flakes as starting materials, respectively. The GO obtained using Hummer's method and simplified Hummer's method had a small area of less than 50 μm 2 and a large area of about 7000 μm 2 , respectively. The small area GO and large area GO were reduced by sodium hydroxide, in which the reduced GO (rGO) with small area and large area were incorporated into a chitosan matrix, respectively, using a simplistic drop-casting technique to produce a thin film. Glass transition temperature (T g ) and mechanical strength of chitosan/large area rGO at concentrations of 0.3, 0.6 and 0.9 wt.% of GO were found to be better than chitosan/small area rGO. We also investigated the T g and mechanical strength between chitosan/small area GO and chitosan/small area rGO, where we discovered that the former had better thermal and tensile properties. By comparing the T g and mechanical strength of chitosan/small area GO against chitosan/large area GO, we found that the latter displayed superior thermal and tensile properties. Antibacterial tests were performed on the graphene-based chitosan composites and their ability to act as bactericide was manifested in the retardation of the growth of Pseudomonas aeruginosa. These composite materials with excellent thermal, tensile and antimicrobial properties find real-life applications in the physical, chemical, mechanical, electrical and bioengineering fields.

show abstract

Section: Antibacterial Susceptibility Testingmentioning

confidence: 99%

Facile preparation of graphene-based chitosan films: Enhanced thermal, mechanical and antibacterial properties

Lim

Huang

Loo

2012

Journal of Non-Crystalline Solids

139

View full text Add to dashboard Cite

show abstract

“…Protein formulations that contain APs are critical for many therapeutic and vaccine applications. Preservatives are also required for drug delivery systems such as injection pens that are used in multiple doses 5 , minipumps that are used for continuous infusion 6 , and topical applications for wound healing 7 .…”

Section: Introductionmentioning

confidence: 99%

Mechanisms of m-cresol-induced Protein Aggregation Studied Using a Model Protein Cytochrome c

Singh

Hutchings

Mallela

2011

Journal of Pharmaceutical Sciences

View full text Add to dashboard Cite

Multi-dose protein formulations require an effective antimicrobial preservative (AP) to inhibit microbial growth during long-term storage of unused formulations. m-cresol is one such AP, but has been shown to cause protein aggregation. However, the fundamental physical mechanisms underlying such AP-induced protein aggregation are not understood. In this study, we used a model protein cytochrome c to identify the protein unfolding that triggers protein aggregation. m-cresol induced cytochrome c aggregation at preservative concentrations that are commonly used to inhibit microbial growth. Addition of m-cresol decreased the temperature at which the protein aggregated and increased the aggregation rate. However, m-cresol did not perturb the tertiary or secondary structure of cytochrome c. Instead, it populated an “invisible” partially unfolded intermediate where a local protein region around the methionine residue at position 80 was unfolded. Stabilizing the Met80 region drastically decreased the protein aggregation, which conclusively shows that this local protein region acts as an aggregation “hot-spot”. Based on these results, we propose that APs induce protein aggregation by partial rather than global unfolding. Because of the availability of site-specific probes to monitor different levels of protein unfolding, cytochrome c provided a unique advantage in characterizing the partial protein unfolding that triggers protein aggregation.

show abstract

Mechanism for benzyl alcohol‐induced aggregation of recombinant human interleukin‐1 receptor antagonist in aqueous solution

Zhang

Roy

Jones

et al. 2004

Journal of Pharmaceutical Sciences

103

View full text Add to dashboard Cite

Untitled

Cited by 9 publications

References 7 publications

Facile preparation of graphene-based chitosan films: Enhanced thermal, mechanical and antibacterial properties

Facile preparation of graphene-based chitosan films: Enhanced thermal, mechanical and antibacterial properties

Mechanisms of m-cresol-induced Protein Aggregation Studied Using a Model Protein Cytochrome c

Mechanism for benzyl alcohol‐induced aggregation of recombinant human interleukin‐1 receptor antagonist in aqueous solution

Contact Info

Product

Resources

About