Nitrous oxide (N 2 O) is a long-lived greenhouse gas (GHG). Its global warming potential is ∼265 times that of CO 2 over a 100 year time horizon (IPCC, 2013). In addition, N 2 O is a dominant ozone-depleting substance that is not regulated by the Montreal Protocol (Ravishankara et al., 2009). On a global scale, natural soils are the largest source of atmospheric N 2 O (H. Tian et al., 2020). Exogenous nitrogen (N), such as the deposited reactive N, is transformed during the nitrification and denitrification processes and partly fixed by the ecosystem through microbial immobilization, plant absorption, and soil storage (Chapin et al., 2011). The unfixed nitrogen (N) leaves the ecosystem in a dissolved form through leaching or gaseous form to the atmosphere (Figure S1 in Supporting Information S1). Overall, N 2 O is a by-product of N transformation and fixation processes in natural soils.