Black chokeberry (<i>Aronia melanocarpa</i> L.) polyphenols attenuate obesity-induced colonic inflammation by regulating gut microbiota and the TLR4/NF-κB signaling pathway in high fat diet-fed rats

Zhu, Yue; Cai, Peng-ju; Dai, Han-chu; Xiao, Yu-hang; Jia, Cheng-li; Sun, Ai-dong

doi:10.1039/d3fo02177g

Cited by 14 publications

(2 citation statements)

References 69 publications

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“…The expression of tight junction proteins such as ZO-1, Occludin and Claudin1, resulting in increased intestinal barrier permeability and elevated blood LPS levels. LPS can activate the TLR4/MyD88/NF- κ B pathway to trigger TNF- α , MCP-1 and IL-6 release (L. Shi et al, 2022 ; Zhu et al, 2023 ). The increasing serum LPS, TNF- α , MCP-1 and IL-6 levels mean the low-grade systemic inflammation in MetS rats.…”

Section: Discussionmentioning

confidence: 99%

Xiasangju alleviate metabolic syndrome by enhancing noradrenaline biosynthesis and activating brown adipose tissue

He,

An,

Shi

et al. 2024

Front. Pharmacol.

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Metabolic syndrome (MetS) is a clinical condition associated with multiple metabolic risk factors leading to type 2 diabetes mellitus and other metabolic diseases. Recent evidence suggests that modulating adipose tissue to adaptive thermogenesis may offer therapeutic potential for MetS. Xiasangju (XSJ) is a marketed drug and dietary supplement used for the treatment of metabolic disease with anti-inflammatory activity. This study investigated the therapeutic effects of XSJ and the underlying mechanisms affecting the activation of brown adipose tissue (BAT) in MetS. The results revealed that XSJ ameliorated MetS by enhancing glucose and lipid metabolism, leading to reduced body weight and abdominal circumference, decreased adipose tissue and liver index, and improved blood glucose tolerance. XSJ administration stimulated catecholamine biosynthesis, increasing noradrenaline (NA) levels and activating NA-mediated proteins in BAT. Thus, BAT enhanced thermogenesis and oxidative phosphorylation (OXPHOS). Moreover, XSJ induced changes in gut microbiota composition, with an increase in Oscillibacter abundance and a decrease in Bilophila, Candidatus Stoquefichus, Holdemania, Parasutterella and Rothia. XSJ upregulated the proteins associated with intestinal tight junctions corresponding with lower serum lipopolysaccharide (LPS), tumor necrosis factor α (TNF-α) monocyte chemoattractant protein-1 (MCP-1) and interleukin-6 (IL-6) levels to maintain NA signaling transport. In summary, XSJ may alleviate MetS by promoting thermogenesis in BAT to ultimately boost energy metabolism through increasing NA biosynthesis, strengthening intestinal barrier integrity and reducing low-grade inflammation. These findings suggest XSJ has potential as a natural therapeutic agent for the treatment of MetS.

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Section: Discussionmentioning

confidence: 99%

Xiasangju alleviate metabolic syndrome by enhancing noradrenaline biosynthesis and activating brown adipose tissue

He,

An,

Shi

et al. 2024

Front. Pharmacol.

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“…Inhibiting the expression of related inflammatory factors by blocking the TLR4 signaling pathway may have a preventive effect on metabolic diseases [17][18][19]. Obesity-induced inflammation is mainly activated by the TLR4 signaling pathway, which in turn activates the downstream pathway nuclear factor kappa B (NF-κB) and causes the production of pro-inflammatory factor TNF-α, further aggravating the inflammation [20,21]. Studies have demonstrated that TLR4 deficiency can alleviate inflammation and insulin resistance in adipose tissue, and inhibit liver steatosis [22].…”

Section: Introductionmentioning

confidence: 99%

Inhibition of Hyperglycemia and Hyperlipidemia by Blocking Toll-like Receptor 4: Comparison of Wild-Type and Toll-like Receptor 4 Gene Knockout Mice on Obesity and Diabetes Modeling

Zhao,

Zheng,

Wang

et al. 2024

Biology

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Innate immune receptor TLR4 plays an important role in glycolipid metabolism. The objective of this study is to investigate the inhibitory effects of blocking TLR4 on hyperglycemia and hyperlipidemia by comparing WT and TLR4−/− mice in obesity and diabetes modeling. The knockout of the TLR4 gene could prevent weight gain induced by a high-fat diet (HFD)/high-sugar and high-fat diet (HSHFD), and the differences in the responses existed between the sexes. It extends the time required to reach the obesity criteria. However, when mice were injected with intraperitoneal streptozotocin (STZ) after being fed by HSHFD for two months, TLR4−/− mice exhibited less weight loss than WT. Blocking TLR4 alleviated the changes in body weight and blood glucose, consequently reducing the efficiency of diabetes modeling, especially for male mice. Additionally, male TLR4−/− obese mice exhibit lower total cholesterol (TC) and low-density lipoprotein (LDL) levels in serum and less formation of fat droplets in the liver compared to WT. On the other hand, the knockout of TLR4 significantly increased the high-density lipoprotein (HDL) of male mice. This study should provide new insights into the role of TLR4, as well as opportunities to target novel approaches to the prevention and treatment of metabolic diseases like obesity and diabetes.

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Catabolism of phenolics from grape peel and its effects on gut microbiota during in vitro colonic fermentation

Cui,

Chen,

Sun

et al. 2024

J Sci Food Agric

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BackgroundGrape peels, the main by‐products of wine processing, are rich in bioactive ingredients of phenolics, including proanthocyanidins, flavonoids and anthocyanins. Phenolics have the function of regulating intestinal microbiota and promoting intestinal health. From the perspective of the dietary nutrition of grape peel phenolics (GPP), this study was to investigate the influence of GPP on the composition and metabolism of human gut microbiota during in vitro fermentation.ResultsThe results indicated that GPP could decrease pH and promote the production of short‐chain fatty acids (SCFAs). ACE and Chao1 indices in GPP group were lower than that of the Blank group. GPP enhanced the levels of Lachnospiraceae UCG‐004, Bacteroidetes, and Roseburia, but reduced the Firmicutes/Bacteroidetes ratio. KEGG enrichment pathways related to phenolic acid metabolism mainly included flavonoid, anthocyanin, flavone and flavonol biosynthesis. Gut microbiota could accelerate the release and breakdown of phenolic compounds, resulting in a decrease in the content of hesperetin‐7‐O‐glucoside, delphinidin‐3‐O‐glucoside, and cyanidin‐3‐rutinoside etc. In vitro antibacterial test found that GPP increased the diameters of the inhibition zones of Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) in a dose‐dependent manner.ConclusionThe above results revealed that GPP might be a potential prebiotic‐like to prevent diseases by improving gut health. This study could provide theoretical basis for potential to exploit GPP as dietary nutrition to maintain intestinal function.This article is protected by copyright. All rights reserved.

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Black chokeberry (Aronia melanocarpa L.) polyphenols attenuate obesity-induced colonic inflammation by regulating gut microbiota and the TLR4/NF-κB signaling pathway in high fat diet-fed rats

Abstract: This study investigated the potential benefits of black chokeberry polyphenols (BCPs) supplementation on lipopolysaccharide (LPS)-stimulated inflammatory response in RAW264.7 cells and obesity-induced colonic inflammation in high-fat diet (HFD)-fed rats model....

Cited by 14 publications

References 69 publications

Xiasangju alleviate metabolic syndrome by enhancing noradrenaline biosynthesis and activating brown adipose tissue

Xiasangju alleviate metabolic syndrome by enhancing noradrenaline biosynthesis and activating brown adipose tissue

Inhibition of Hyperglycemia and Hyperlipidemia by Blocking Toll-like Receptor 4: Comparison of Wild-Type and Toll-like Receptor 4 Gene Knockout Mice on Obesity and Diabetes Modeling

Catabolism of phenolics from grape peel and its effects on gut microbiota during in vitro colonic fermentation

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