Black grain eumycetoma due to Diaporthe ueckerae. Taxonomical update of previous agents of infections due to Diaporthe spp.

Cabeza, Matías Sebastián; Gómez, Abel; Sasoni, Natalia; Gamarra, Soledad; García‐Effrón, Guillermo

doi:10.1016/j.mmcr.2022.11.001

Cited by 2 publications

(4 citation statements)

References 14 publications

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“…duobushaemulonii (NR_130694.1), Candida tolerans (GeneBank accession number # NR_155009.1), Candida pseudoflosculorum (aa.# NR_165968.1), and Clavispora reshetovae (aa.#NR_137723.1), respectively, leading to an incorrect identification of our strains. The opposite situation was observed when attempting to identify a eumycetoma agent recently 32 . Hence, these kinds of discrepancies should be taken into consideration when identifying stains using molecular tools.…”

Section: Discussionmentioning

confidence: 96%

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Mechanism of azole resistance in Candida vulturna, an emerging multidrug resistant pathogen related with Candida haeumulonii and Candida auris

Macedo,

Berrio,

Escandon

et al. 2024

Mycoses

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BackgroundCandida vulturna is an emerging pathogen belonging to the Metshnikowiaceae family together with Candida auris and Candida haemulonii species complex. Some strains of this species were reported to be resistant to several antifungal agents.ObjectivesThis study aims to address identification difficulties, evaluate antiungal susceptibilities and explore the molecular mechanisms of azole resistance of Candida vulturna.MethodsWe studied five C. vulturna clinical strains isolated in three Colombian cities. Identification was performed by phenotypical, proteomic and molecular methods. Antifungal susceptibility testing was performed following CLSI protocol. Its ERG11 genes were sequenced and a substitution was encountered in azole resistant isolates. To confirm the role of this substitution in the resistance phenotype, Saccharomyces cerevisiae strains with a chimeric ERG11 gene were created.ResultsDiscrepancies in identification methods are highlighted. Sequencing confirmed the identification as C. vulturna. Antifungal susceptibility varied among strains, with four strains exhibiting reduced susceptibility to azoles and amphotericin B. ERG11 sequencing showed a point mutation (producing a P135S substitution) that was associated with the azole‐resistant phenotype.ConclusionsThis study contributes to the understanding of C. vulturna's identification challenges, its susceptibility patterns, and sheds light on its molecular mechanisms of azole resistance.

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Section: Discussionmentioning

confidence: 96%

“…The opposite situation was observed when attempting to identify a eumycetoma agent recently. 32 Hence, these kinds of discrepancies should be taken into consideration when identifying stains using molecular tools.…”

Section: Discussionmentioning

confidence: 99%

Mechanism of azole resistance in Candida vulturna, an emerging multidrug resistant pathogen related with Candida haeumulonii and Candida auris

Macedo,

Berrio,

Escandon

et al. 2024

Mycoses

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“…While primarily affecting plants and associated with various plant diseases (e.g., grapes, soybean, sunflower, citrus, peach, tea mango, dragon fruit, rambutan, coffee bean, blue trumpet vine, red silk cotton, and makha) [9][10][11][12], Diaporthe species are rarely linked to human infections. These infections are typically opportunistic, occurring in individuals with compromised immune systems, such as those with organ transplants, HIV/AIDS, poorly controlled diabetes, or other immunosuppressive conditions [13].…”

Section: Discussionmentioning

confidence: 99%

“…These infections often manifest as localized skin or soft tissue infections, such as mycetoma and soft tissue ulcers, which are usually curable [13]. There has been only one reported case of PD-related peritonitis due to Diaporthe.…”

Section: Discussionmentioning

confidence: 99%

Peritonitis Caused by Various Species of Diaporthe in Peritoneal Dialysis Patients: A Plant Pathogen to Human Infection

Halue,

Chieochanthanakij,

Kittipanyaworakun

et al. 2024

Cureus

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Peritonitis caused by dematiaceous molds is uncommon but poses a significant threat to patients undergoing peritoneal dialysis (PD), leading to high mortality and morbidity. This report highlights three cases of peritonitis caused by three distinct species of Diaporthe (D. amygdali, D. eucalyptorum, and D. phaseolorum), initially unidentified through conventional culture methods. The nucleotide sequences of internal transcribed spacer regions (ITS), 18S nuclear ribosomal small subunit (SSU), and 28S nuclear ribosomal large subunit (LSU) of the ribosomal DNA gene correctly identified the isolates. Despite early catheter removal and administration of appropriate antifungal medications, all patients experienced fatal outcomes. DNA barcoding emerges as a valuable tool for accurately diagnosing species within the genus of pathogenic microbes, aiding in identifying the root causes of infections. It emphasizes the importance of strict adherence to aseptic techniques during PD exchanges to prevent peritonitis caused by plant-borne pathogens.

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Black grain eumycetoma due to Diaporthe ueckerae. Taxonomical update of previous agents of infections due to Diaporthe spp.

Cited by 2 publications

References 14 publications

Mechanism of azole resistance in Candida vulturna, an emerging multidrug resistant pathogen related with Candida haeumulonii and Candida auris

Mechanism of azole resistance in Candida vulturna, an emerging multidrug resistant pathogen related with Candida haeumulonii and Candida auris

Peritonitis Caused by Various Species of Diaporthe in Peritoneal Dialysis Patients: A Plant Pathogen to Human Infection

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