Black mulberry extract inhibits hepatic adipogenesis through AMPK/mTOR signaling pathway in T2DM mice

Yao, Yudi; Chen, Yang; Chen, Huijian; Pan, Xin; Li, Xiaojun; Liu, Wenqi; Bahetjan, Yerlan; Lu, Binan; Pang, Kejian; Yang, Xinzhou; Pang, Zongran

doi:10.1016/j.jep.2023.117216

Cited by 7 publications

(6 citation statements)

References 49 publications

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“…Subsequently, compound 6 was determined to be 8R, and by calculating the ECD curves, both experimental and calculated values showed a consistent Cotton effect at 216 and 229 nm (Figure 6). Dichotomin G (7) was separated as a yellow oil and had a molecular formula of C 12 H 16 O 4 based on a hydrogen-adduct ion peak (m/z 225.1120 [M + H] + ; calcd. 225.1121).…”

Section: ■ Results and Discussionmentioning

confidence: 99%

“…The specific procedure for separation and purification can be found in the Supporting Information. Dichotomin G (7). Yellow oil; [α] D 25 −6.0 (c 0.5, MeOH); UV (MeOH) λ max (log ε): 225 (1.47), 280 (0.38) nm; ECD (0.5 mg/mL, MeOH) λ max (Δε): 215 (0.72), 253 (−0.37) nm; IR (KBr) ν max : 3393, 1718, 1517, 1448, 1378, 1232, 1171, 1103, 1023, 921 cm −1 ; 1 H and 13 C NMR, see Table 2 − for 301.1081).…”

Section: ■ Materials and Methodsmentioning

confidence: 99%

“…After myotubes were fully differentiated, the cells continued to be differentiated and cultured in normal control (0.1% DMSO) and compound 3 (10, 20, and 30 μg/mL) for 48 h. After washing the cells with PBS for 3 rinses, the proteins of L6 cells were isolated, scraped off with a spatula, and then lysed using the proper kit. Relevant proteins were extracted from L6 cells based on previously reported methods. , Protein samples were divided into aliquots and then subjected to electrophoresis on a 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). When the sample is electrophoresed to the bottom of the gel, stop electrophoresis and immediately transfer the membrane.…”

Section: Methodsmentioning

confidence: 99%

“…Relevant proteins were extracted from L6 cells based on previously reported methods. 7,28 Protein samples were divided into aliquots and then subjected to electrophoresis on a 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). When the sample is electrophoresed to the bottom of the gel, stop electrophoresis and immediately transfer the membrane.…”

Section: Characterization Of Compounds Dichotominmentioning

confidence: 99%

“…Recently, increasing interest has been paid to the development of new drugs for the treatment of T2DM using natural products due to their high potency, low toxicity, and availability. The medicinal and edible natural products exhibit clear diabetes-fighting benefits. − Overall, natural products from plants and functional plant foods have an increasingly important potential value and application in the prevention and treatment of T2DM.…”

Section: Introductionmentioning

confidence: 99%

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Phenolic Constituents with Glucose Uptake and GLUT4 Translocation Bioactivities from the Fruits of Cordia dichotoma

Wei,

Zhang,

Kang

et al. 2024

J. Agric. Food Chem.

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From the fruits of Cordia dichotoma, 11 new phenolic compounds, dichotomins A-K, were isolated, together with 19 known compounds. Through the analysis of detailed NMR data and HRESIMS data, the planar structures of all compounds were confirmed. Using NMR calculations, the absolute configuration of dichotomins A-K was elucidated by comparing their observed and computed electronic circular dichroism (ECD) spectra. Dichotomin H (8) and dichotomin I (9) were determined as two pairs of enantiomers. The enantiomers of compounds 8 and 9 were separated using chiral-phase high-performance liquid chromatography (HPLC), and the stereostructure of each enantiomer was determined by similarly calculating the ECD. Compounds 3, 5, 7, 17, 18, 23−25, and 27−30 increased glucose uptake by 1.04-to 2.85-folds at concentrations of 30 μg/mL. Further studies revealed that compounds 3 and 5 had a moderate effect on glucose transporter 4 (GLUT4) translocation activity in L6 cells. At 30 μg/mL, compound 3 significantly enhanced AMPK phosphorylation and GLUT4 expression. As a whole, compound 3 has the potential to be a drug candidate for the treatment of type 2 diabetes mellitus (T2DM).

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Section: ■ Results and Discussionmentioning

confidence: 99%

Section: ■ Materials and Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Characterization Of Compounds Dichotominmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Phenolic Constituents with Glucose Uptake and GLUT4 Translocation Bioactivities from the Fruits of Cordia dichotoma

Wei,

Zhang,

Kang

et al. 2024

J. Agric. Food Chem.

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Exploring the mechanisms of Cornus officinalis in the treatment of non-alcoholic steatohepatitis using network pharmacology, molecular docking and molecular dynamics simulations

Guo,

Wan,

Wang

2024

Discov Appl Sci

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Non-alcoholic steatohepatitis (NASH) is a predominant metabolic liver disease, typically characterized by hepatic steatosis, oxidative stress, and inflammation. The traditional Chinese medicine Cornus officinalis possesses anti-inflammatory and hepatoprotective pharmacological properties and has shown ameliorative effects on NASH. however, its mechanism of action remains unclear. This study aims to elucidate the mechanisms by which C. officinalis ameliorates NASH. The active components of C. officinalis were analyzed using the Traditional Chinese Medicine Systems Pharmacology Database (TCMSP), and the corresponding targets were predicted. Subsequently, the DisGeNET, GeneCards, and GEO databases were employed to identify NASH-related targets. Venn diagrams were used to intersect the C. officinalis targets with the NASH targets. Protein–protein interaction (PPI) networks were constructed using the STRING database, and PPI network analysis was performed using Cytoscape. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses were conducted using the Database for Annotation, Visualization, and Integrated Discovery (DAVID), followed by molecular docking validation. Cornus officinalis was found to contain 20 major active ingredients corresponding to 672 potential targets, 61 of which overlapped with NASH targets. PPI network, GO, and KEGG pathway analyses identified four targets with the highest correlation, and molecular docking results indicated that the active ingredients of C. officinalis exhibited strong binding affinities to NASH targets. The treatment of NASH with C. officinalis is characterized by multiple active ingredients and multiple targets, underscoring the major advantage of traditional Chinese medicine in treating NASH.

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Functional benefits of mulberry leaf tea or extracts to alleviate metabolic diseases: Current opinion and perspectives

James,

Wang,

Chen

et al. 2024

Food Bioscience

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Black mulberry extract inhibits hepatic adipogenesis through AMPK/mTOR signaling pathway in T2DM mice

Cited by 7 publications

References 49 publications

Phenolic Constituents with Glucose Uptake and GLUT4 Translocation Bioactivities from the Fruits of Cordia dichotoma

Phenolic Constituents with Glucose Uptake and GLUT4 Translocation Bioactivities from the Fruits of Cordia dichotoma

Exploring the mechanisms of Cornus officinalis in the treatment of non-alcoholic steatohepatitis using network pharmacology, molecular docking and molecular dynamics simulations

Functional benefits of mulberry leaf tea or extracts to alleviate metabolic diseases: Current opinion and perspectives

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