Black phosphorus-incorporated novel Ti-12Mo-10Zr implant for multimodal treatment of osteosarcoma

Abstract

The repair and reconstruction of large bone defects after bone tumor resection is still a great clinical challenge. At present, orthopedic implant reconstruction is the mainstream treatment for repairing bone defects. However, according to clinical feedback, local tumor recurrence and nonunion of bone graft are common reasons leading to the failure of bone defect repair and reconstruction after bone tumor resection, which seriously threaten the physical and mental health of patients. On this basis, here the selfdeveloped low modulus Ti-12Mo-10Zr alloy (TMZ) was chosen as substrate material. To improve its biological activity and osteointegration, calcium, oxygen, and phosphorus co-doped microporous coating was prepared on TMZ alloy by microarc oxidation (MAO). Then, black phosphorus (BP) nanosheets were incorporated onto MAO treated TMZ alloy to obtain multifunctional composites. The obtained BP-MAO-TMZ implant exhibited excellent photothermal effects and effective ablation of osteosarcoma cancer cells under the irradiation of 808 nm near infrared laser, while no photothermal or therapeutic effects were observed for TMZ alloy. Meanwhile, the structure/component bionic coating obtained after MAO treatment as well as the P-driven in situ biomineralization performance after incorporation of BP nanosheets endowed BP-MAO-TMZ implant with synergistic promoting effect on MC3T3-E1 osteoblasts' activity, proliferation and differentiation ability. This study is expected to provide effective clinical solutions for problems of di cult bone regeneration and tumor recurrence after tumor resection in patients with bone tumors, and to solve a series of medical problems such as poor prognosis and poor postoperative quality of patients life with malignant bone tumors.

The Biological Impact of Some Phosphonic and Phosphinic Acid Derivatives on Human Osteosarcoma

“…In addition, new research is employing a biocompatible and biodegradable phosphorus isomer-black phosphorus-which exhibits a unique molecular structure that further assures the optic and electronic characteristics to enable its biomedical applications for alternative therapies in osteosarcoma treatment, such as photothermal therapy, drug delivery and 3D printing [41][42][43], resorting to different approaches, such as the following: (i) the development of 3D-printed scaffolds reinforced with black phosphorus nanosheets for an exquisite treatment of osteosarcoma, providing the necessary tools for both photothermal ablation of osteosarcoma and bone regeneration under physiological conditions [44]; (ii) the use of black phosphorus quantum dots to obtain a drug delivery platform that could be further used for both chemotherapy and photothermal treatment of osteosarcoma [45] or development of an injectable chitosan-derived hydrogel containing black phosphorus nanosheets and doxorubicin for both chemo-and photothermal therapy [46]; and (iii) the design of black phosphorus nanosheet multifunctional composites to provide effective ablation of osteosarcoma by means of a photothermal approach [47].…”