Black rat (Rattus rattus) genomic variability characterized by chromosome painting

Cavagna, Pietro; Stone, G.; Stanyon, Roscoe

doi:10.1007/s0033501-1020-7

Cited by 12 publications

(18 citation statements)

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“…However, some species with conserved genomes have been identified. For example, a single chromosomal rearrangement distinguishes Apodemus (Matsubara et al, 2004;Stanyon et al, 2004) and relatively high genome conservation was established for species within Rattus and Tokudaia, representatives of the tribe Rattini (Guilly et al, 1999;Stanyon et al, 1999;Cavagna et al, 2002;Nakamura et al, 2007;Badenhorst et al, 2011).…”

Section: Muridaementioning

confidence: 99%

Chromosomal evolution in Rodentia

et al. 2011

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Rodentia is the most species-rich mammalian order and includes several important laboratory model species. The amount of new information on karyotypic and phylogenetic relations within and among rodent taxa is rapidly increasing, but a synthesis of these data is currently lacking. Here, we have integrated information drawn from conventional banding studies, recent comparative painting investigations and molecular phylogenetic reconstructions of different rodent taxa. This permitted a revision of several ancestral karyotypic reconstructions, and a more accurate depiction of rodent chromosomal evolution.

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Section: Muridaementioning

confidence: 99%

Chromosomal evolution in Rodentia

et al. 2011

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“…To date, four major chromosomal groups of black rats have been identified: an 'Asian' group with 2n = 42, an 'Oceanian' group with 2n = 38, a 'Ceylonese' group with 2n = 40, and a 'Mauritius' type with a distinct 2n = 42. A recent study (Cavagna et al, 2002) using the chromosome painting technique suggested a higher level of karyotypic diversity than that detected by conventional G-and Q-banding studies (Yosida, 1980).…”

Section: Introductionmentioning

confidence: 99%

Preliminary genetic characterization of two lineages of black rats (Rattus rattus sensu lato) in Japan, with evidence for introgression at several localities

et al. 2005

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We conducted a pilot survey of genetic diversity among 37 karyotyped individuals of the black rat Rattus rattus (sensu lato) from six localities on the Japanese Islands, using complete gene sequences of mitochondrial cytochrome b (cyt b ) and nuclear interphotoreceptor retinoid binding protein (IRBP). Our sampling included two previously documented karyotypic groups: 'Oceanian' with 2n = 38 and 'Asian' with 2n = 42. Cyt b sequences for most individuals clustered according to their karyotypic groups, with an average between-group divergence of 3.8%. One exception was that individuals from Kagoshima (Kyushu Island) showed 'Asian' karyotypes combined with a cyt b haplotype that differed by a single nucleotide substitution from the haplotype of the 'Oceanian' karyotypic group. Six IRBP haplotypes were identified. They belonged to three distinct IRBP lineages (I-III), with an average inter-lineage divergence of 1%. Among homozygous individuals, these lineages showed good association with the karyotypic groups: IRBP lineage I occurred only with 'Oceanian' karyotypes, while IRBP lineages II and III both occurred with 'Asian' karyotypes. Individuals from Kagoshima all possessed IRBP of 'Asian' lineages, despite the presence of an 'Oceanian' mitochondrial type. The Chichijima population (Ogasawara Islands) featured exclusively 'Asian' karyotypes and cyt b sequences, but various combinations of all three IRBP lineages. The Kagoshima and Chichijima populations thus provide strong evidence of viable hybridization and genetic introgression between the two karyotypic groups, but with variable genetic outcomes. Our results demonstrate the potential of combined analysis of karyotypes and mitochondrial and nuclear gene sequences to elucidate the complex dispersal and population history of the black rat.

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“…In particular, comparative studies of their karyotypes remain scarce. Although the relationship between mouse and rat genomes has been extensively investigated by chromosome painting (Grutzner et al 1999;Guilly et al 1999;Stanyon et al 1999;Helou et al 2001;Nilsson et al 2001;Cavagna et al 2002), somewhat surprisingly, no comparisons have been conducted between Rattus species and their allies.…”

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Chromosomal evolution in Rattini (Muridae, Rodentia)

et al. 2011

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The Rattini (Muridae, Murinae) includes the biologically important model species Rattus norvegicus (RNO) and represents a group of rodents that are of clinical, agricultural and epidemiological importance. We present a comparative molecular cytogenetic investigation of ten Rattini species representative of the genera Maxomys, Leopoldamys, Niviventer, Berylmys, Bandicota and Rattus using chromosome banding, cross-species painting (Zoofluorescent in situ hybridization or FISH) and BAC-FISH mapping. Our results show that these taxa are characterised by slow to moderate rates of chromosome evolution that contrasts with the extensive chromosome restructuring identified in most other murid rodents, particularly the mouse lineage. This extends to genomic features such as NOR location (for example, NORs on RNO 3 are present on the corresponding chromosomes in all species except Bandicota savilei and Niviventer fulvescens, and the NORs on RNO 10 are conserved in all Rattini with the exception of Rattus). The satellite I DNA family detected and characterised herein appears to be taxon (Rattus) specific, and of recent origin (consistent with a feedback model of satellite evolution). BACmapping using clones that span regions responsible for the morphological variability exhibited by RNO 1, 12 and 13 (acrocentric/submetacentric) and their orthologues in Rattus species, demonstrated that the differences are most likely due to pericentric inversions as exemplified by data on Rattus tanezumi. Chromosomal characters detected using R. norvegicus and Maxomys surifer whole chromosome painting probes were mapped to a consensus sequencebased phylogenetic tree thus allowing an objective assessment of ancestral states for the reconstruction of the putative Rattini ancestral karyotype. This is thought to have comprised 46 chromosomes that, with the exception of a single pair of metacentric autosomes, were acrocentric in morphology.

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Black rat (Rattus rattus) genomic variability characterized by chromosome painting

Cited by 12 publications

References 0 publications

Chromosomal evolution in Rodentia

Chromosomal evolution in Rodentia

Preliminary genetic characterization of two lineages of black rats (Rattus rattus sensu lato) in Japan, with evidence for introgression at several localities

Chromosomal evolution in Rattini (Muridae, Rodentia)

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