Black Sigatoka: An Increasing Threat to Banana Cultivation

Marin, D. H.; Romero, R.; Guzmán, M.; Sutton, T. B.

doi:10.1094/pdis.2003.87.3.208

Cited by 229 publications

(277 citation statements)

References 9 publications

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“…This fruit constitutes a major staple food for millions of people in developing countries, and it is also considered the highest selling fruit in the world market, making this crop a very important export commodity for the producing countries (Jones, 2000;FAO, 2005). At the present time, one of the most significant constraints of banana production that causes significant economic losses are numerous environmental challenges, particularly with fungal and bacterial pathogens as well as pests and abiotic stresses (Agrios, 1997;Carlier et al, 2000;Marín et al, 2003). This situation is exacerbated by the limited diversity of cultivars.…”

Section: Introductionmentioning

confidence: 99%

Isolation and characterization of nucleotide-binding site and C-terminal leucine-rich repeat-resistance gene candidates in bananas

Lu¹,

Xu²,

Xie³

et al. 2011

Genet. Mol. Res.

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ABSTRACT. Commercial banana varieties are highly susceptible to fungal pathogens, as well as bacterial pathogens, nematodes, viruses, and insect pests. The largest known family of plant resistance genes encodes proteins with nucleotide-binding site (NBS) and C-terminal leucine-rich repeat (LRR) domains. Conserved motifs in such genes in diverse plant species offer a means for the isolation of candidate genes in banana that may be involved in plant defense. Six degenerate PCR primers were designed to target NBS and additional domains were tested on commercial banana species Musa acuminata subsp malaccensis and the Musa AAB Group propagated in vitro and plants maintained in a greenhouse. Total DNA was isolated by a modified CTAB extraction technique. Four resistance gene analogs were amplified and deposited in GenBank and assigned numbers HQ199833-HQ199836. The predicted amino acid sequences compared to the amino acid sequences of known resistance genes (MRGL1, MRGL2, MRGL3, and MRGL4) revealed significant sequence similarity. The presence of consensus do- NBS-LRR-resistance gene candidates in Musa mains, namely kinase-1a, kinase-2 and hydrophobic domain, provided evidence that the cloned sequences belong to the typical non-Toll/interleukin-1 receptor-like domain NBS-LRR gene family.

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Section: Introductionmentioning

confidence: 99%

Isolation and characterization of nucleotide-binding site and C-terminal leucine-rich repeat-resistance gene candidates in bananas

Lu¹,

Xu²,

Xie³

et al. 2011

Genet. Mol. Res.

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“…Banana represents a very important staple food commodity in the tropics after wheat, rice and corn (Marin et al, 2003;Ploetz, 2000). Banana plants are susceptible to a variety of devastating diseases, including those in the Mycosphaerella leaf spot complex, caused by three related ascomycetous fungi.…”

Section: Introductionmentioning

confidence: 99%

“…It later spread to replace M. musicola as the most important constraint in many banana-producing areas. However, Mycospaerella musicola still remains a serious pathogen of banana at higher altitudes, cooler temperatures and subtropical regions (Marin et al, 2003;Jones, 2002). In the mid-1990s, M. eumusae was recognised as the cause of a new Sigatoka-like disease of banana (Crous and Mourichon, 2002;Carlier et al, 2000).…”

Section: Introductionmentioning

confidence: 99%

Molecular Diagnostics in the Mycosphaerella Leaf Spot Disease Complex of Banana and for Radopholus Similis

Arzanlou¹,

Kema²,

Waalwijk³

et al. 2009

Acta Hortic.

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Mycosphaerella leaf spots and nematodes threaten banana cultivation worldwide. The Mycosphaerella disease complex involves three related ascomycetous fungi: Mycosphaerella fijiensis, M. musicola and M. eumusae. The exact distribution of these three species and their disease epidemiology remain unclear, since their symptoms and life cycles are rather similar. Diagnosing these diseases and the respective causal agents is based on the presence of host symptoms and fungal fruiting structures, but is time consuming and not conducive to preventive management. In the present study, we developed rapid and robust species-specific diagnostic tools to detect and quantify M. fijiensis, M. musicola and M. eumusae. Conventional species-specific PCR primers were developed based on the actin gene that detected as little as 100, 1 and 10 pg/µl DNA from, respectively, M. fijiensis, M. musicola and M. eumusae. Furthermore, TaqMan real-time quantitative PCR assays that were developed based on the β-tubulin gene detected quantities as low as 1 pg/µl DNA of each species from pure cultures and 1.6 pg/µl DNA/mg of M. fijiensis from dry leaf tissue. The efficacy of the tests was validated using naturally infected banana leaves. Similar technology has been used to develop a quantitative PCR assay for the banana burrowing nematode, Radopholus similis, which is currently being validated.

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“…The worldwide production of banana and plantain is estimated to 106 Mt per year 1 , of which 15 Mt are intended for the exportation markets and the remainder in food producing culture or for the supply to the local markets. The banana and plantain productions, which contribute to the basic nutrition closely of 400 M of individuals in the world, are subject to an increasing pressure of the diseases, of which the principal is the Black Leaf Streak Disease [1].…”

Section: Introductionmentioning

confidence: 99%

“…The banana and plantain productions, which contribute to the basic nutrition closely of 400 M of individuals in the world, are subject to an increasing pressure of the diseases, of which the principal is the Black Leaf Streak Disease [1].…”

Section: Introductionmentioning

confidence: 99%

In liquid medium colchicine treatment induces non chimerical doubled-diploids in a wide range of mono- and interspecific diploid banana clones

Bakry¹,

Reberdiere²,

Pichot³

et al. 2007

Fruits

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In liquid medium colchicine treatment induces non chimerical doubleddiploids in a wide range of mono-and interspecific diploid banana clones.Abstract --Introduction. The worldwide production of banana and plantain, which is estimated to 106 Mt·year -1 , relies on a narrow genetic basis. Thus, banana production is fragile regarding emergent diseases, and creation of new varieties of banana stands out as a real necessity for its sustainability. In banana, various improvement strategies aim to create triploid hybrids. The objective of this report is to present a simple in vitro methodology to induce stable tetraploid plants by colchicine treatment, usable as parent for obtaining triploid varieties. Materials and methods. Twenty-one diploid M. acuminata clones and three interspecific M. acuminata / M. balbisiana diploid clones were treated as proliferating culture in liquid medium with 1.25 mM of colchicine for 48 h. Plant screening has been performed by morphological identification in greenhouse, chromosome counts on root tips and flow cytometry on leave blades on vitroplants using propidium iodide staining. Results. Chromosome counts led to distinguish diploid and tetraploid plants but did not afford to detect chimeras. Flow cytometry allowed an early screening of a larger number of plants leading to detect rapidly chimerical plants. It was observed that some stable 2x / 4x cytochimeras are periclinal. Tetraploid clones were obtained with all diploid genotypes. General behaviour of tetraploids in the field was globally weaker than the corresponding diploids. Nevertheless, all the doubled-diploids flowered and crossed with diploid plants to obtain triploid progenies. Conclusion. This study has clearly shown that induction of stable doubled-diploid plants can be obtained from a wide range of genetically different bananas. These results open the way to the systematic use of doubled-diploids by banana breeding programs for the release of enhanced triploid varieties.Guadeloupe / Musa / tissue culture / colchicine / chromosome number / tetraploidy / chimeras La colchicine appliquée en milieu liquide induit des diploïdes doublés non chimériques dans une large gamme de clones de bananiers diploïdes mono ou interspécifiques.Résumé --Introduction. La production mondiale de bananes et plantains, qui est estimée à 106 Mt·an -1 , s'appuie sur une base génétique étroite. La production de bananes est donc fragile quant à la menace des maladies émergentes et la création de nouvelles variétés apparaît comme une réelle nécessité pour sa pérennité. Les diverses stratégies d'amélioration du bananier visent à créer des hybrides triploïdes. L'objectif de cet article est de présenter une méthode aisée de production de plantes tétraploïdes stables par traitement de colchicine appliqué in vitro, utilisables comme parents pour obtenir de nouvelles variétés hybrides triploïdes. Matériel et méthodes. Vingt-et-un clones diploïdes de M. acuminata et trois clones diploïdes interspécifiques M. acuminata / M. balbisiana ont été traités durant la ...

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Black Sigatoka: An Increasing Threat to Banana Cultivation

Cited by 229 publications

References 9 publications

Isolation and characterization of nucleotide-binding site and C-terminal leucine-rich repeat-resistance gene candidates in bananas

Isolation and characterization of nucleotide-binding site and C-terminal leucine-rich repeat-resistance gene candidates in bananas

Molecular Diagnostics in the Mycosphaerella Leaf Spot Disease Complex of Banana and for Radopholus Similis

In liquid medium colchicine treatment induces non chimerical doubled-diploids in a wide range of mono- and interspecific diploid banana clones

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