Black soybean seed coat polyphenols prevent AAPH-induced oxidative DNA-damage in HepG2 cells

Abstract

Black soybean seed coat extract (BE), which contains abundant polyphenols such as procyanidins, cyanidin 3-glucoside, (+)-catechin, and (−)­epicatechin, has been reported on health beneficial functions such as antioxidant activity, anti-inflammatory, anti-obesity, and anti-diabetic activities. In this study, we investigated that prevention of BE and its polyphenols on 2,2'-azobis(2-methylpropionamide) dihydrochloride (AAPH)-induced oxidative DNA damage, and found that these polyphenols inhibited AAPH-induced formation of 8-hydroxy-2'-deoxyguanosine (8-OHdG) as a biomarker for oxidative DNA damage in HepG2 cells. Under the same conditions, these polyphenols also inhibited AAPH-induced accumulation of reactive oxygen species (ROS) in the cells. Inhibition of ROS accumulation was observed in both cytosol and nucleus. It was confirmed that these polyphenols inhibited formation of AAPH radical using oxygen radical absorbance capacity assay under the cell-free conditions. These results indicate that polyphenols in BE inhibit free radical-induced oxidative DNA damages by their potent antioxidant activity. Thus, BE is an effective food material for prevention of oxidative stress and oxidative DNA damages.

The Evaluation of Dietary Black Soybean and Purple Sweet Potato on Insulin Sensitivity in Streptozotocin - Induced Diabetic Rats

“…23 Furthermore, BSB active compound suppresses oxidative damage in DNA by neutralizing free radical in cytosol and nucleus. 24 In addition, PSP rich-anthocyanins contents prevent free radical of 2,2-diphenyl-1-picrylhydrazyl (DPPH) and superoxide radical. 25 The combination of BSB and PSP reduce MDA concentration in DM rats.…”

Black Soybean Improves Vascular Function and Blood Pressure: A Randomized, Placebo Controlled, Crossover Trial in Humans

“…The detailed method is written in the later section ( Section 2.8 , Extraction and Quantification of Polyphenols.) For the antioxidant activity, the 2, 2′-azobis (2-amidinopropane) dihydrochloride (AAPH) radical absorbance capacity of black soybean and placebo cookies was measured [ 9 ].…”

“…In addition, isoflavones are major polyphenols in the grain of black soybean, as they are in the yellow one. Previous studies reported that polyphenols in the black soybean had various beneficial physiological functions, such as antioxidant in HepG2 cells [ 9 ], anti-obesity, and anti-diabetic activities in C57BL/6 mice [ 10 ].…”

Synthesis, Radical-Scavenging Activities, and Protective Effects against AAPH-Induced Oxidative Damage in DNA and Erythrocytes of Piperine Derivatives

“…AAPH can react with oxygen in the air to produce peroxy radicals (ROO_) at a steady rate. ROO_ can capture the hydrogen atom supplied by the C-4′ atom in DNA, which could result in the DNA molecule unwinding and generating a variety of carbonyl-containing small molecular substances [23,28]. ese substances can react with TBA to produce thiobarbituric acid reactive species (TBARS, λ max � 535 nm) under acidic conditions.…”