Black tea extract prevents inorganic arsenic induced uncontrolled proliferation, epithelial to mesenchymal transition and induction of metastatic properties in HaCaT keratinocytes - an in vitro study

Ghosh, Archismaan; Mukherjee, Sutapa; Roy, Madhumita

doi:10.1016/j.tiv.2022.105478

Cited by 3 publications

(4 citation statements)

References 29 publications

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“…Similar observations have been reported in in vitro studies, where BTE showed its potential to reverse EMT and repress cell migration in human oral carcinoma cells (SCC4) [25]. BTE was also found to inhibit chronic iAs-induced cell transformation and induction of EMT in HaCaT cells [29]. BTE also elicits a prominent role in preventing iAs-induced epigenetic modulations in Swiss albino mice, exhibiting its chemopreventive potential [8].…”

Section: Discussionsupporting

confidence: 81%

“…ROS may also act as signalling intermediates engaged in cell proliferation, angiogenesis, EMT and metastasis [33]. iAs exposure to HaCaT cells led to excess ROS generation and induction of EMT [29]. Higher expression of TGF-β has been found in iAs treated group of mice, compared to the control group.…”

Section: Discussionmentioning

confidence: 99%

“…Report from our laboratory showed that black tea extract (BTE) effectively quenched iAs-induced ROS production [7], and mitigated epigenetic modulations, preventing carcinogenesis in Swiss albino mice [8]. BTE was found to inhibit iAs-induced EMT in skin keratinocyte (HaCaT) cells, thus exhibiting its chemopreventive potential in an in vitro model [29]. BTE, by virtue of its antioxidant potential, may prevent iAs-induced EMT in Swiss albino mice.…”

Section: Introductionmentioning

confidence: 99%

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Black Tea Extract, via Modulation of TGF-β Pathway, Prevents Inorganic Arsenic-induced Development of Squamous Cell Carcinoma of the Skin in Swiss Albino Mice

Ghosh

Roy

2023

J Cancer Prev

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Chronic exposure to inorganic arsenic (iAs) elevates reactive oxygen species (ROS) generation and up-regulates TGF-β signalling. This promotes induction of epithelial to mesenchymal transition (EMT) and causes the development of squamous cell carcinoma (SCC) of skin. Black tea is a popular beverage worldwide and an effective antioxidant. Chemopreventive potential of black tea extract (BTE) against iAs induced carcinogenicity has been explored here. The study aims to investigate the role of BTE in prevention of iAs-induced SCC of skin in Swiss albino mice via the modulation of TGF-β signalling and EMT. Mice were divided into (1) control, (2) iAs, (3) iAs+BTE, and (4) BTE groups and were administered iAs and BTE alone, or in combination for 330 days. Histological studies were performed to assess development of SCC. ROS generation was estimated by flowcytometry. Expression of TGF-β and downstream proteins belonging to suppressor of mothers against decapentaplegic (Smad), phosphoinositide-3-kinase (PI3K)-protein kinase B (AKT) and mitogen-activated protein kinase (MAPK) pathways was assessed by immunoblotting. Expression of EMT markers was evaluated by immunoblotting, immunohistochemistry and semi-quantitative reverse transcriptase-PCR. After 330 days of iAs treatment, development of invasive SCC of skin probably due to excess ROS generation, elevation of TGF-β, downregulation of the Smad pathway, upregulation of PI3K-AKT and MAPK signalling molecules and induction of EMT was observed. All these modulations were found to be reversed by BTE, which inhibits iAs induced SCC of skin by quenching excess ROS, promoting Smad mediated TGF-β signalling, downregulating signalling intermediates of PI3K-AKT and MAPK pathways and inhibiting EMT.

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Section: Discussionsupporting

confidence: 81%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Black Tea Extract, via Modulation of TGF-β Pathway, Prevents Inorganic Arsenic-induced Development of Squamous Cell Carcinoma of the Skin in Swiss Albino Mice

Ghosh

Roy

2023

J Cancer Prev

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“…However, tumor formation in the SCID mice was transient, which brought the authors to conclude that chronic exposure to low-level arsenic concentrations led to the development of a mild malignant phenotype that may require other factors (e.g., UV radiation or mechanic stress) for a full-blown malignant transformation. Interestingly, the in vitro long-term exposure approach was also applied to identify the chemopreventive ability of black tea extract (BTE) in a BTE and arsenic coexposure scenario [117]. In that study, skin keratinocyte cells (HaCaTs) exposed to arsenic for 34 weeks showed clear signs of transformation, while cells coexposed to arsenic and BTE for the same period did not develop such evident malignant features.…”

Section: Representative Studies Using the Proposed Approach To Evalua...mentioning

confidence: 99%

In Vitro Approaches to Determine the Potential Carcinogenic Risk of Environmental Pollutants

Barguilla

Maguer-Satta

Guyot

et al. 2023

IJMS

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One important environmental/health challenge is to determine, in a feasible way, the potential carcinogenic risk associated with environmental agents/exposures. Since a significant proportion of tumors have an environmental origin, detecting the potential carcinogenic risk of environmental agents is mandatory, as regulated by national and international agencies. The challenge mainly implies finding a way of how to overcome the inefficiencies of long-term trials with rodents when thousands of agents/exposures need to be tested. To such an end, the use of in vitro cell transformation assays (CTAs) was proposed, but the existing prevalidated CTAs do not cover the complexity associated with carcinogenesis processes and present serious limitations. To overcome such limitations, we propose to use a battery of assays covering most of the hallmarks of the carcinogenesis process. For the first time, we grouped such assays as early, intermediate, or advanced biomarkers which allow for the identification of the cells in the initiation, promotion or aggressive stages of tumorigenesis. Our proposal, as a novelty, points out that using a battery containing assays from all three groups can identify if a certain agent/exposure can pose a carcinogenic risk; furthermore, it can gather mechanistic insights into the mode of the action of a specific carcinogen. This structured battery could be very useful for any type of in vitro study, containing human cell lines aiming to detect the potential carcinogenic risks of environmental agents/exposures. In fact, here, we include examples in which these approaches were successfully applied. Finally, we provide a series of advantages that, we believe, contribute to the suitability of our proposed approach for the evaluation of exposure-induced carcinogenic effects and for the development of an alternative strategy for conducting an exposure risk assessment.

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The potential for black tea in combating arsenic toxicity

Roy,

Ghosh,

Datta

2025

Tea in Health and Disease Prevention

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Black tea extract prevents inorganic arsenic induced uncontrolled proliferation, epithelial to mesenchymal transition and induction of metastatic properties in HaCaT keratinocytes - an in vitro study

Cited by 3 publications

References 29 publications

Black Tea Extract, via Modulation of TGF-β Pathway, Prevents Inorganic Arsenic-induced Development of Squamous Cell Carcinoma of the Skin in Swiss Albino Mice

Black Tea Extract, via Modulation of TGF-β Pathway, Prevents Inorganic Arsenic-induced Development of Squamous Cell Carcinoma of the Skin in Swiss Albino Mice

In Vitro Approaches to Determine the Potential Carcinogenic Risk of Environmental Pollutants

The potential for black tea in combating arsenic toxicity

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