Black-Tea Polyphenols Suppress Postprandial Hypertriacylglycerolemia by Suppressing Lymphatic Transport of Dietary Fat in Rats

Abstract

Administration of black-tea polyphenols (BTP) at 100 and 200 mg/kg of body weight in rats suppressed postprandial hypertriacylglycerolemia in a dose-dependent manner. Administration of BTP also suppressed lymphatic recovery of (14)C-trioleoylglycerol in rats that were cannulated in the thoracic duct. BTP dose-dependently inhibited the activity of pancreatic lipase in vitro with an IC50 of 0.254 mg/mL. When purified theaflavins, which are components of BTP, were used, theaflavins with galloyl moieties, but not those without galloyl moiety, inhibited the activity of pancreatic lipase. Theaflavin-3,3'-digallate (TFDG) was more effective in inhibiting the activity of pancreatic lipase than epigallocatechin gallate (EGCG), epicatechin gallate (ECG), and a mixture of EGCG and ECG. BTP and TFDG had a similar effect in inhibiting the activity of pancreatic lipase when the total polyphenol amount was adjusted to the same. BTP had no effect on micellar solubility of hydrolysis products of triacylglycerol. These results suggest that BTP suppressed postprandial hypertriacylglycerolemia by reducing triacylglycerol absorption via the inhibition of pancreatic lipase activity.

Inhibition of pancreatic lipase by black tea theaflavins: Comparative enzymology and in silico modeling studies

“…Inhibition of fat digestion and absorption has been implicated as a potential mechanism and previous studies have shown that tea polyphenols have inhibitory activity against PL (Grove, et al, 2012; Klaus, Pultz, Thone-Reineke, & Wolfram, 2005; Kobayashi, et al, 2009; Sae-Tan, Grove, Kennett, & Lambert, 2011; Wolfram, Raederstorff, Wang, Teixeira, Elste, & Weber, 2005). In spite of this, black tea theaflavins have been understudied.…”

“…Black tea polyphenols have been shown to significantly reduce serum triglyceride concentration in rats after co-administration of a liquid fat test meal and black tea extract compared to control treated rats (Kobayashi, Ichitani, Suzuki, Unno, Sugawara, Yamahira, et al, 2009; Uchiyama, Taniguchi, Saka, Yoshida, & Yajima, 2011). Mice fed a high fat diet supplemented with 5% black tea polyphenol extract had significantly less body weight gain, parametrial adipose tissue mass, and liver lipid content compared to mice fed a high-fat diet (Uchiyama, et al, 2011).…”

In vitro bioaccessibility, bioavailability and plasma protein interaction of polyphenols from Annurca apple (M. pumila Miller cv Annurca)

“…Other works reveal the possibility that oligomeric procyanidins may be effective in reducing plasma cholesterol concentration and suppressing activity on postprandial hypertriacylglycerolemia. Actually, such effects were mainly demonstrated for grape seed, red wine and tea polyphenolic extracts (Leifert & Abeywardena, 2008;Ikeda, Yamahira, Kato, & Ishikawa, 2010;Kobayashi et al, 2009), but no studies reporting such bioactivities for apple procyanidins are available in literature so far.…”

Proanthocyanidins Modulate MicroRNA Expression in Human HepG2 Cells

“…Moreover, GSPE contains considerable amounts of galloylated monomers, such as epicatechin gallate and epigallocatechin gallate [20], whereas CPE contains little amounts of epigallocatechin [21]. Differences in the chemical structures of the proanthocyanidins present in the extract, such as the degree of polymerization and/or the presence of galloyl moieties, have been demonstrated to be important for proanthocyanidin functions [22], [23], [24]. For example, galloylated polyphenols have been shown to have greater inhibitory effects than non-galloylated polyphenols on pancreatic lipase [22].…”