Bladder augmentation using allogenic bladder submucosa seeded with cells

Yoo, James J.; Meng, Jiao; Oberpenning, F.; Atala, Anthony

doi:10.1016/s0090-4295(97)00644-4

Cited by 445 publications

(254 citation statements)

References 18 publications

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“…Tissue engineering is being explored to provide a gut-free augmentation tissue, with early promising animal results using the bladder acellular matrix graft with and without urothelial and smooth muscle cell seeding [160,161]. Other alternatives being developed are small intestinal submucosa as a scaffold for normal urothelial and detrusor ingrowth [162], and progressive percutaneous dilatation for bladder tissue expansion [163]; again, promising early results were reported in animal studies.…”

Section: The Futurementioning

confidence: 99%

Augmentation cystoplasty

Greenwell¹,

Venn²,

Mundy³

2001

BJU International

195

137

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Section: The Futurementioning

confidence: 99%

Augmentation cystoplasty

Greenwell¹,

Venn²,

Mundy³

2001

BJU International

195

137

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“…2 The initial applications of the decellularization matrix have been demonstrated only for the hollow organs or thin layer of tissues, such as bladder, artery, esophagus, trachea, and skin. [3][4][5][6][7] Ott et al reported that they constructed a heart by using a rat's decellularized heart scaffold. 8 This study became the milestone in the field of reengineering whole organs using a decellularized scaffold.…”

Section: Introductionmentioning

confidence: 99%

Hepatocyte Culture in Autologous Decellularized Spleen Matrix

Gao¹,

Xiang³

et al. 2015

Organogenesis

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ABSTRACT. Background and Aims: Using decellularized scaffold to reengineer liver tissue is a promising alternative therapy for end-stage liver diseases. Though the decellularized human liver matrix is the ideal scaffold for reconstruction of the liver theoretically, the shortage of liver donors is still an obstacle for potential clinical application. Therefore, an appropriate alternative scaffold is needed. In the present study, we used a tissue engineering approach to prepare a rat decellularized spleen matrix (DSM) and evaluate the effectiveness of this DSM for primary rat hepatocytes culture. Methods: Rat decellularized spleen matrix (DSM) was prepared by perfusion of a series of detergents through spleen vasculature. DSM was characterized by residual DNA and specific extracellular matrix distribution. Thereafter, primary rat hepatocytes were cultured in the DSM in a 3-dimensional dynamic culture system, and liver cell survival and biological functions were evaluated by comparison with 3-dimensional sandwich culture and also with cultured in decellularized liver matrix (DLM). Results: Our research found that DSM did not exhibit any cellular components, but preserved the main extracellular matrix and the intact vasculature evaluated by DNA detection, histology, immunohistochemical staining, vessel corrosion cast and upright metallurgical microscope. *Correspondence to: Yi Lv; Email: luyi169@126.com Received July 7, 2014; Revised September 10, 2014; Accepted January 18, 2015.16 Organogenesis, 11:16-29, 2015 Ó 2015 Taylor & Francis Group, LLC ISSN: 1547-6278 print / 1555-8592 online DOI: 10.1080/15476278.2015 Moreover, the method of DSM preparation procedure was relatively simple with high success rate (100%). After seeding primary hepatocytes in DSM, the cultured hepatocytes survived inside DSM with albumin synthesis and urea secretion within 10 d. Additionally, hepatocytes in dynamic culture medium had better biological functions at day 10 than that in sandwich culture. Albumin synthesis was 85.67 § 6.34 mg/10 7 cell/24h in dynamic culture in DSM compared to 62.43 § 4.59 mg/10 7 cell/ 24h in sandwich culture (P < 0.01) and to 87.54 § 5.25 mg/10 7 cell/24h in DLM culture (P > 0.05); urea release was 32.14 § 8.62 mg/10 7 cell/24h in dynamic culture in DSM compared to 20.47 § 4.98 mg/10 7 cell/24h in sandwich culture (P < 0.05) and to 37.38 § 7.29 mg/10 7 cell/24h cultured in DLM (P > 0.05). Conclusion: The present study demonstrates that DSM can be prepared successfully using a tissue engineering approach. The DSM is an appropriate scaffold for primary hepatocytes culture.

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“…As membranas biológicas, como bexiga homóloga (Baiotto, 2001;Oliveira, 1999), pericárdio bovino (Kambic et al, 1992), placenta de humanos (Fishman et al, 1987) e submucosa de bexiga (Yoo et al, 1998), foram testadas na reconstrução da bexiga de cães com resultados variados e, às vezes, conflitantes.…”

Section: Introductionunclassified

Avaliação clínica e histopatológica de alo-transplante de bexiga em cães

Teixeira

Rezende²,

Júnior

et al. 2007

Arq. Bras. Med. Vet. Zootec.

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RESUMOTestou-se a hipótese de o alo-transplante de bexiga devolver a capacidade estrutural e funcional desse órgão, usando-se 10 cães machos, saudáveis, submetidos à cistectomia parcial com preservação do trígono vesical. Utilizou-se o alo-transplante na reconstrução da vesícula urinária com acompanhamento dos animais durante 60 dias. Aos oito dias de pós-operatório, os cães apresentavam capacidade de contenção urinária e micção espontânea. Houve aumento gradativo do volume da vesícula urinária, alcançando, aos 60 dias, valores significativamente mais altos que os observados antes da cirurgia. Verificou-se integração tecidual com regeneração parcial na interface do transplante, caracterizada por reconstituição epitelial, proliferação fibroblástica, neoangiogênese e surgimento de fibras musculares lisas, aos 60 dias. Problemas como rejeição e obstrução uretral ocorreram em três cães. Conclui-se que o alo-transplante de bexiga em cães é viável e devolve a capacidade de repleção e as demais funções fisiológicas da vesícula urinária, ocorrendo regeneração parcial dos tecidos aos 60 dias de pós-operatório. Palavras-chave: cão, alo-transplante, bexiga ABSTRACT The hypothesis that urinary bladder allotransplant in dogs repairs

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Bladder augmentation using allogenic bladder submucosa seeded with cells

Cited by 445 publications

References 18 publications

Augmentation cystoplasty

Augmentation cystoplasty

Hepatocyte Culture in Autologous Decellularized Spleen Matrix

Avaliação clínica e histopatológica de alo-transplante de bexiga em cães

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