Bladder Squamous Cell Carcinomas Express Psoriasin and Externalize it to the Urine

Celis, Julio E.; Rasmussen, Hanne H.; Vorum, Henrik; Madsen, Peder; Honoré, Bent; Wolf, Hans; Ørntoft, Torben F.

doi:10.1016/s0022-5347(01)66118-4

Cited by 135 publications

(91 citation statements)

References 25 publications

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“…S100A2 has been reported to both promote tumor growth (42) and function as a tumor suppressor gene (40,43). S100A7 is overexpressed in breast cancer (44), epithelial skin tumors (45), bladder cancer (46), and is markedly elevated in lesions from psoriasis patients (47), suggesting a role in keratinocyte differentiation, and in regulating the innate immune response associated with epithelial inflammation (46). S100A12, a potent monocyte chemoattractant (48) that mediates allergic inflammation by Table 3.…”

Section: Discussionmentioning

confidence: 99%

Immune Dysregulation and Tumor-Associated Gene Changes in Recurrent Respiratory Papillomatosis: A Paired Microarray Analysis

DeVoti

Rosenthal

et al. 2008

Mol Med

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Recurrent respiratory papillomas (RRP) are benign airway tumors, caused primarily by human papillomaviruses (HPV) types 6 and 11. The disease is characterized by multiple recurrences after surgical removal, with limited effective therapy. To identify novel targets for future therapy, we established transcriptional profiles for actively growing papillomas compared with autologous, clinically normal, laryngeal epithelia (adjacent tissue). Total ribonucleic acid (RNA) from 12 papillomas and 12 adjacent tissues were analyzed by microarray, and the matched sets of tissues compared by paired t test, to identify differentially expressed genes in papilloma tissues while minimizing variations intrinsic to individual patients. Quantitative polymerase chain reaction (PCR) was used to confirm the relative expression levels for a subset of genes. Within the 109 differentially expressed transcripts whose expression varied at least three-fold were two large groups of genes with related functions. The first group consisted of 18 genes related to host defense, including both innate and adaptive immunity. The second group contained 37 genes that likely contribute to growth of papillomas as benign tumors, since the altered pattern of expression also had been reported previously in many cancers. Our results support our previous studies that document a systemic T H 2-like adaptive immune response in RRP, and suggest that there is a role for altered innate immunity in RRP as well. We propose that HPV 6 and 11 infection establishes a tumorigenic microenvironment characterized by alteration of both innate inflammatory signals and adaptive immune responses that prevent effective T H 1-like response, in conjunction with altered expression of numerous genes that regulate cellular growth and differentiation.

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Section: Discussionmentioning

confidence: 99%

Immune Dysregulation and Tumor-Associated Gene Changes in Recurrent Respiratory Papillomatosis: A Paired Microarray Analysis

DeVoti

Rosenthal

et al. 2008

Mol Med

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“…Although transitional cell carcinoma has been the chief target of biomarker discovery and validation studies, some work using 2-D electrophoresis of tissue samples has focused on the rarer squamous cell carcinoma (60,65). The experiments have identified psoriasin as a potential biomarker protein that is present in urine from squamous cell carcinoma patients but is less frequently detectable in urine from transitional cell carcinoma patients (60).…”

Section: Urinary Proteomics In Cancer Diagnosismentioning

confidence: 99%

“…The experiments have identified psoriasin as a potential biomarker protein that is present in urine from squamous cell carcinoma patients but is less frequently detectable in urine from transitional cell carcinoma patients (60). In the remainder of this section, we focus on the more common transitional cell carcinoma of the bladder.…”

Section: Urinary Proteomics In Cancer Diagnosismentioning

confidence: 99%

Discovery of Urinary Biomarkers

Pisitkun

Johnstone

Knepper

2006

Molecular & Cellular Proteomics

366

318

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A myriad of proteins and peptides can be identified in normal human urine. These are derived from a variety of sources including glomerular filtration of blood plasma, cell sloughing, apoptosis, proteolytic cleavage of cell surface glycosylphosphatidylinositol-linked proteins, and secretion of exosomes by epithelial cells. Mass spectrometry-based approaches to urinary protein and peptide profiling can, in principle, reveal changes in excretion rates of specific proteins/peptides that can have predictive value in the clinical arena, e.g. in the early diagnosis of disease, in classification of disease with regard to likely therapeutic responses, in assessment of prognosis, and in monitoring response to therapy. These approaches have potential value, not only in diseases of the kidney and urinary tract but also in systemic diseases that are associated with circulating small protein and peptide markers that can pass the glomerular filter. Most large scale biomarker discovery studies reported thus far have used one of two approaches to identify proteins and peptides whose excretion in urine changes in specific disease states: 1) two-dimensional electrophoresis with mass spectrometric and/or immunochemical identification of proteins and 2) top-down mass spectrometric methods (SELDI-TOF-MS and capillary electrophoresis-MS). These studies have been chiefly in the areas of nephrology, urology, and oncology. We review these applications, focusing on two areas of progress, viz.

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“…Originally associated with abnormally differentiating keratinocytes in psoriasis, ''psoriasin'' (S100A7) has since been found to be expressed in association with neoplasia in several tissues, including squamous carcinomas in skin (2,3) and bladder (4), as well as adenocarcinomas of the breast (5,6). Although the protein is not expressed in normal luminal epithelium, increased expression occurs in parallel with early stages of tumor progression, and S100A7 can be among the most highly expressed proteins in preinvasive carcinoma in situ in both skin (3,7) and breast (8,9).…”

Section: Introductionmentioning

confidence: 99%

The S100A7-c-Jun Activation Domain Binding Protein 1 Pathway Enhances Prosurvival Pathways in Breast Cancer

et al. 2005

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S100A7 is among the most highly expressed genes in preinvasive breast cancer, is a marker of poor survival when expressed in invasive disease, and promotes breast tumor progression in experimental models. To explore the mechanism of action, we examined the role of S100A7 in cell survival and found that overexpression of S100A7 in MDA-MB-231 cell lines promotes survival under conditions of anchorageindependent growth. This effect is paralleled by increased activity of nuclear factor-KB (3-fold) and phospho-Akt (4-fold), which are known to mediate prosurvival pathways. S100A7 and phospho-Akt are also correlated in breast tumors examined by immunohistochemistry (n = 142; P < 0.0001; r = 0.34). To explore the underlying mechanism, we examined the role of a putative c-Jun activation domain-binding protein 1 (Jab1)-binding domain within S100A7 using a panel of MDA-MB-231 breast cell lines stably transfected with either S100A7 or S100A7 mutated at the Jab1 domain. Structural analysis by three-dimensional protein modeling, immunoprecipitation, and yeast two-hybrid assay and functional analysis using transfected reporter gene and Western blot assays revealed that the in vitro effects of S100A7 on phospho-Akt and the nuclear factor-KB pathway are dependent on the Jab1-binding site and the interaction with Jab1. Enhanced epidermal growth factor receptor signaling was also found to correlate with the increased phospho-Akt. Furthermore, the Jab1-binding domain is also necessary for the enhanced tumorigenicity conferred by S100A7 expression in murine xenograft tumors in vivo. We conclude that the S100A7-Jab1 pathway acts to enhance survival under conditions of cellular stress, such as anoikis, which may promote progression of breast cancer. (Cancer Res 2005; 65(13): 5696-702)

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Bladder Squamous Cell Carcinomas Express Psoriasin and Externalize it to the Urine

Abstract: The results point towards psoriasin, alone or as part of a biomarker profile, as a potential marker for the noninvasive follow-up of patients with SCC.

Cited by 135 publications

References 25 publications

Immune Dysregulation and Tumor-Associated Gene Changes in Recurrent Respiratory Papillomatosis: A Paired Microarray Analysis

Immune Dysregulation and Tumor-Associated Gene Changes in Recurrent Respiratory Papillomatosis: A Paired Microarray Analysis

Discovery of Urinary Biomarkers

The S100A7-c-Jun Activation Domain Binding Protein 1 Pathway Enhances Prosurvival Pathways in Breast Cancer

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