Blast resistance gene Pi54 over-expressed in rice to understand its cellular and sub-cellular localization and response to different pathogens

Singh, Jaya; Gupta, Santosh Kumar; Devanna, B. N.; Singh, Sunil Kumar; Upadhyay, Avinash; Sharma, Tilak Raj

doi:10.1038/s41598-020-59027-x

Cited by 21 publications

(16 citation statements)

References 96 publications

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“…These qualitative and major R genes have been extensively used in blast resistance breeding programs worldwide ( conducted to investigate the subcellular localization of Pi54 with Green Fluorescent Protein (GFP) as a molecular tag. This is the first detailed report, which emphasizes the cellular and subcellular distribution of the broad-spectrum blast resistance gene Pi54 in rice and the impact of its constitutive expression towards resistance against other fungal and bacterial pathogens of rice [166]. These R genes function in a gene-for-gene fashion, meaning that for every R gene in the host, there is an Avr gene in the pathogen.…”

Section: Resistance Genes and Qtls For Rice Blast Diseasementioning

confidence: 96%

Rice Blast Disease in India: Present Status and Future Challenges

Annegowda¹,

Prasannakumar²,

Mahesh³

et al. 2022

Integrative Advances in Rice Research

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Rice (Oryza sativa L.) is the staple food of the majority of Indians, and India is both the major producer and consumer of rice. Rice cultivation in India is confronted with diverse agro-climatic conditions, varying soil types, and several biotic and abiotic constraints. Among major fungal diseases of Rice in India, the blast caused by Magnaporthe oryzae is the most devastating disease, with the neck blast being the most destructive form. Most of the blast epidemic areas in India have been identified with a mixture of races blast fungus resulting in the resistance breakdown in a short period. At present, a more significant number of the rice varieties cultivated in India were bred by conventional breeding methods with blast resistance conferred by a single resistance gene. Therefore, the blast disease in India is predominantly addressed by the use of ecologically toxic fungicides. In line with the rest of the world, the Indian scientific community has proven its role by identifying several blast resistance genes and successfully pyramiding multiple blast resistance genes. Despite the wealth of information on resistance genes and the availability of biotechnology tools, not a great number of rice varieties in India harbor multiple resistance genes. In the recent past, a shift in the management of blast disease in India has been witnessed with a greater focus on basic research and modern breeding tools such as marker-assisted selection, marker-assisted backcross breeding, and gene pyramiding.

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Section: Resistance Genes and Qtls For Rice Blast Diseasementioning

confidence: 96%

Rice Blast Disease in India: Present Status and Future Challenges

Annegowda¹,

Prasannakumar²,

Mahesh³

et al. 2022

Integrative Advances in Rice Research

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“…Pi54 confers broad spectrum, durable resistance against M. oryzae infection [ 95 , 165 ]. The functional role of Pi54 in blast resistance was validated using transgenic and RNAi-mediated approach [ 165 , 166 , 167 , 168 , 169 ]. Subsequently, orthologs of Pi54 gene; Pi54rh and Pi54of, also show broad spectrum and overlapping patterns of resistance against multiple strains of M. oryzae [ 89 , 170 , 171 ].…”

Section: Molecular Interplay Between Rice and M Oryzaementioning

confidence: 99%

Understanding the Dynamics of Blast Resistance in Rice-Magnaporthe oryzae Interactions

Kumar

Jain

Solanke

et al. 2022

JoF

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Rice is a global food grain crop for more than one-third of the human population and a source for food and nutritional security. Rice production is subjected to various stresses; blast disease caused by Magnaporthe oryzae is one of the major biotic stresses that has the potential to destroy total crop under severe conditions. In the present review, we discuss the importance of rice and blast disease in the present and future global context, genomics and molecular biology of blast pathogen and rice, and the molecular interplay between rice–M. oryzae interaction governed by different gene interaction models. We also elaborated in detail on M. oryzae effector and Avr genes, and the role of noncoding RNAs in disease development. Further, rice blast resistance QTLs; resistance (R) genes; and alleles identified, cloned, and characterized are discussed. We also discuss the utilization of QTLs and R genes for blast resistance through conventional breeding and transgenic approaches. Finally, we review the demonstrated examples and potential applications of the latest genome-editing tools in understanding and managing blast disease in rice.

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“…Then after, the conidia produce germ tubes. According to their research findings, [20] stated the ability of mature appressorium to break the leaf cuticle by creating cellular turgor pressure through the accumulation of compatible solutes and secretions of cell wall degrading enzaymes. And hence, it is the beginning of gaining entry in to the epidermal cells.…”

Section: The Biology and Pathogenicity Of Magnaporthe Oryzaementioning

confidence: 99%

Untitled

2022

IJAERDS

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Finger millet is still neglected cereal crop mostly grown and consumed by resourcepoor people mainly in arid and semi-arid tropics. The projected food demand of the global population will pressure plant breeders to secure the demand by improving the yield of major cereal crops. To meet this demand, there is a consistent need to look beyond the conventional farming system to increase production and productivity in sustainable ways. However, finger millet production and productivities are limited by many factors namely biotic and abiotic. Of biotic factors, finger millet blast disease is the most limiting factor. The disease is caused by the filamentous ascomycetous fungus called Magnaporthe oryzae. Under favorable conditions, the pathogen can devastate the entire field in all finger millet growing regions. Moreover, the frequent breakdown of blast resistance causes significant yield losses worldwide. The utilization of innovative and efficient research strategies guarantees future sustainable production of finger millet. Therefore, exploring an advanced breeding methodologies presently being used and discussing future directions for development of blast resistant varieties will help providing a broad understanding concept for finger millet breeding.

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Blast resistance gene Pi54 over-expressed in rice to understand its cellular and sub-cellular localization and response to different pathogens

Cited by 21 publications

References 96 publications

Rice Blast Disease in India: Present Status and Future Challenges

Rice Blast Disease in India: Present Status and Future Challenges

Understanding the Dynamics of Blast Resistance in Rice-Magnaporthe oryzae Interactions

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