Blast resistance in rice: a review of conventional breeding to molecular approaches

Miah, Gous; Rafii, Mohd Y.; Ismail, Mohd Razi; Puteh, Adam; Harun, Abdul Rahim; Asfaliza, R.; Latif, Abdul

doi:10.1007/s11033-012-2318-0

Cited by 211 publications

(144 citation statements)

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“…Various strategies in conventional breeding programs have been used in rice variety improvement, including the combining of several genes to provide durable resistance. However, a major disadvantage of the conventional breeding methods is that it is time consuming, leading to a considerable time lag between the emergence of virulent pathotypes of the causal pathogen and the development of new resistant cultivars (Miah et al 2013). In addition, the pyramiding of blast resistance genes with similar reactions to more than one race or isolate, using conventional breeding methods, is difficult due to the dominance and epistasis effects of genes.…”

Section: Discussionmentioning

confidence: 99%

Pyramiding of four blast resistance QTLs into Thai rice cultivar RD6 through marker-assisted selection

Suwannual¹,

Chankaew²,

Monkham³

et al. 2017

Czech J. Genet. Plant Breed.

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Suwannual T., Chankaew S., Monkham T., Saksirirat W., Sanitchon J. (2017): Pyramiding of four blast resistance QTLs into Thai rice cultivar RD6 through marker-assisted selection. Czech J. Genet. Plant Breed., 53: 1-8.Thai rice cultivar RD6 is well known for its cooking and eating qualities. However it is susceptible to blast disease, a major rice disease caused by the fungus Magnaporthe oryzae. This study focused on the pyramiding of four QTLs for blast resistance located on chromosomes 1, 2, 11 and 12, from two RD6 introgression lines. Marker-assisted selection was performed and facilitated the selection with 8 microsatellite flanking markers to enable the selection in BC 2 F 2:3 lines. All possible combinations of the four QTL alleles were assessed for blast resistance by artificial inoculation using 8 diverse isolates in a greenhouse and under field conditions using the upland short row method. The results showed that the RD6 introgression lines carrying a high number of QTLs for blast resitance achieved from pyramiding have high levels of blast resistance and broad spectrum of resistance to the blast pathogens prevalent in the region. Only one of the M. oryzae isolates, THL185, was virulent to all the breeding lines, suggesting that the identification of new blast resistance genes or QTLs and pyramiding them into RD6 for durable blast resistance and no yield penalty should be the focus of further research.

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Section: Discussionmentioning

confidence: 99%

Pyramiding of four blast resistance QTLs into Thai rice cultivar RD6 through marker-assisted selection

Suwannual¹,

Chankaew²,

Monkham³

et al. 2017

Czech J. Genet. Plant Breed.

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“…Microsatellite marker is the most preferable marker for plant breeding application due to well distributed throughout rice genome and hypervariable (Miah et al, 2013). Marker screening is important to represent the population.…”

Section: Discussionmentioning

confidence: 99%

Introgression of Pi-kh Resistance Gene into a Malaysian Cultivar, MR264 using Marker-Assisted Backcrossing (MABC)

Hasan

Rafii

Harun

et al. 2015

IJAB

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Magnophorthe oryzae is one of the most destructive disease that affected rice productivity. Developing new breeding strategies for durable blast resistant varieties is essential for crop protection. In this study, we aimed to introgress a blast resistance gene using local resistant genotypes, Pongsu Seribu 2 into MR264, a high-yielding rice variety that is blast susceptible thru Marker-Assisted Backcrossing (MABC) approach. Results demonstrated that, resistance gene (Pi-kh) was successfully incorporated into MR264-rice variety. Two foreground markers, RM206 and RM5961 were identified tightly linked to the target gene which located at chromosome 11. Both markers were utilized at each backcrossed generation to identify plants possessing heterozygous alleles for the target gene. A total of 82 background markers were used to evaluate recovery of recurrent parent genome in each backcrossed generation. The percentage of recovery of parent genome in BC1F1 and BC2F1 were 79.1% and 89.7% indicated a higher percentage compared to conventional method. The expected number of resistant and susceptible plants in segregation ratio for a backcrossed model fitted with 1:1 with (p>0.05) was not significantly different form the number of observed plants. This research finding will be helpful guidance of application of MABC in developing a blast resistant variety with highest recovery of RP genome with reducing number of generation.

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“…Genetic improvement of rice for fungus resistance is the need of the day due to vulnerability of this crop to various pathogens, including fungi, bacteria, and viruses (Gust et al, 2010;Miah et al, 2013;Sattari et al, 2014). There are several genes that were isolated and cloned, and then transformation was carried out in rice plants to confer resistance against various species of fungi.…”

Section: Fungus-resistant Ricementioning

confidence: 99%

Genetic improvement of rice for biotic and abiotic stress tolerance

Ansari¹,

Shaheen²,

Bukhari³

et al. 2015

Turk J Bot

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IntroductionRice (Oryza sativa L.) is one of the oldest cultivated crops. It has been cultivated in India and China for several thousands of years (Poehlman and Sleper, 1995). The major cultivated species of rice, Oryza sativa (2n = 2x = 24), originated in southern and southwestern tropical Asia. The other species of cultivated rice, Oryza glaberrima (2n = 2x = 24), is indigenous to the upper valley of the Niger River and it is cultivated only in western tropical Africa. Twenty-three species and 10 recognized genome types (AA, BB, CC, BBCC, CCDD, EE, FF, GG, HHJJ, and HHKK; Gramene: http://www.gramene.org/species/ oryza/rice_taxonomy.html) of Oryza are recognized. Close relatives of O. sativa are the wild perennial species O. rufipogon and the wild annual species O. nivara. Both are diploid weedy species with the AA genome.Rice is an important food crop and it needs continuous improvement due to the continuous increase in population. The major objectives to improve rice crops using biotechnological techniques include: 1) high yield potential, 2) early maturity, 3) resistance to lodging and shattering, 4) resistance to stress environments, 5) disease resistance, 6) insect resistance, 7) grain quality, and 8) enhancement of nutritional components. Economic importance of riceRice (Oryza sativa L.) is the most important crop of world. About 90% of the world's rice is grown in China, India, Pakistan, Japan, Korea, Southeast Asia, and other adjacent areas (USDA, 2014). Outside of Asia, Brazil and the United States produce the largest amounts of rice (Poehlman and Sleper, 1995). Rice is the staple food for over one-half of the world's people (FAO, 2008). In Pakistan, rice is a highly valued food crop and it is also a major export item. It accounts for 3.2% of the total value added in agriculture and 0.7% of the GDP. Area sown for rice is estimated at 2.891 × 10 6 ha and the production was 7.005 × 10 6 t in -2015 (Ministry of Finance, 2015. Rice is cultivated in diversified climatic conditions of Pakistan. Basmati rice (Indica) is grown in the traditional rice-growing belt of Punjab Province. In Swat, in high altitude alpine valleys, temperate Japonica rice is grown. In the south of Khyber Pakhtunkhwa, Sindh and Baluchistan provinces, IRRItype long-grain heat-tolerant tropical rice is mainly grown. Transformation of rice cropsThe world will need about 25% more rice by the year 2030 to meet the estimated demand of an increasing global population (Wani and Sah, 2014). One way to meet this challenge is to grow rice on more area, which is difficult due to increasing urbanization and escalating population in Abstract: Rice (Oryza sativa L.) is among the most important food crops that provide a staple food for nearly half of the world's population. Rice crops are prone to various types of stresses, both biotic and abiotic. Biotic stresses include insect pests, fungus, bacteria, viruses, and herbicide toxicity. Among abiotic stresses, drought, cold, and salinity are also well studied in rice. Various genes have been identified,...

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Blast resistance in rice: a review of conventional breeding to molecular approaches

Cited by 211 publications

References 142 publications

Pyramiding of four blast resistance QTLs into Thai rice cultivar RD6 through marker-assisted selection

Pyramiding of four blast resistance QTLs into Thai rice cultivar RD6 through marker-assisted selection

Introgression of Pi-kh Resistance Gene into a Malaysian Cultivar, MR264 using Marker-Assisted Backcrossing (MABC)

Genetic improvement of rice for biotic and abiotic stress tolerance

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