Blastic Transformation in Leucocyte Cultures

Fine Structural Features of Lymphocyte Transformation Induced by Mercuric Salts

“…An interesting and important difference seems to exist between the ultrastructural changes induced by immunospccific agents such as smallpox vaccine [5] and by non-specific agents such as PHA or mercuric compounds. Obviously, the specific stimulation is able to provoke proliferation of the rough-surfaced endoplasmic reticulum which is not the case for non-specific stimulation.…”

Short Term Culture of Chicken Lymphoid Cells

“…The first manifestations of cell transformation are protein and RNA synthesis [17], followed in turn by the appearance of polyribosomes [18] and increased DNA synthesis [19]. There arc indications that specific anti genic stimulation is associated with production of RNA responses different from that produced by PH A stimulation [20].…”

“…The ultimate cell type produced by in vitro transformation is still undetermined: cells resembling plasma cells arc found in cultures stimulated with vaccinia [18] and 8-12 day cultures stimulated with PYVM contain cells morphologically identical to plasma cells [21]. This has not been seen in PHA treated cultures.…”