Blastococcus capsensis sp. nov., isolated from an archaeological Roman pool and emended description of the genus Blastococcus, B. aggregatus, B. saxobsidens, B. jejuensis and B. endophyticus

Hezbri, Karima; Louati, Moussa; Nouioui, Imen; Gtari, Maher; Rohde, Manfred; Spröer, Cathrin; Schümann, Peter; Klenk, Hans‐Peter; Ghodhbane‐Gtari, Faten; Montero‐Calasanz, Maria del Carmen

doi:10.1099/ijsem.0.001443

Cited by 40 publications

(42 citation statements)

References 48 publications

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“…Typically associated with rocks or marine environments 270,271 ; an isolate was obtained from human stool 272 ; Blastococcus was identified in 1 placental microbiota study at low abundance (<0.01%). 69 Yes 29…”

Section: Blastococcusmentioning

confidence: 99%

Does the human placenta delivered at term have a microbiota? Results of cultivation, quantitative real-time PCR, 16S rRNA gene sequencing, and metagenomics

Theis

Romero

Winters

et al. 2019

American Journal of Obstetrics and Gynecology

215

203

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BACKGROUND: The human placenta has been traditionally viewed as sterile, and microbial invasion of this organ has been associated with adverse pregnancy outcomes. Yet, recent studies that utilized sequencing techniques reported that the human placenta at term contains a unique microbiota. These conclusions are largely based on the results derived from the sequencing of placental samples. However, such an approach carries the risk of capturing background-contaminating DNA (from DNA extraction kits, polymerase chain reaction reagents, and laboratory environments) when low microbial biomass samples are studied. OBJECTIVE: To determine whether the human placenta delivered at term in patients without labor who undergo cesarean delivery harbors a resident microbiota ("the assemblage of microorganisms present in a defined niche or environment"). STUDY DESIGN: This cross-sectional study included placentas from 29 women who had a cesarean delivery without labor at term. The study also included technical controls to account for potential backgroundcontaminating DNA, inclusive in DNA extraction kits, polymerase chain reaction reagents, and laboratory environments. Bacterial profiles of placental tissues and background technical controls were characterized and compared with the use of bacterial culture, quantitative real-time polymerase chain reaction, 16S ribosomal RNA gene sequencing, and metagenomic surveys. RESULTS: (1) Twenty-eight of 29 placental tissues had a negative culture for microorganisms. The microorganisms retrieved by culture from the remaining sample were likely contaminants because corresponding 16S ribosomal RNA genes were not detected in the same sample.(2) Quantitative real-time polymerase chain reaction did not

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Section: Blastococcusmentioning

confidence: 99%

Does the human placenta delivered at term have a microbiota? Results of cultivation, quantitative real-time PCR, 16S rRNA gene sequencing, and metagenomics

Theis

Romero

Winters

et al. 2019

American Journal of Obstetrics and Gynecology

215

203

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“…The order Geodermatophilales ( Sen et al, 2014 ) comprises the sole family Geodermatophilaceae , which was initially proposed by Normand et al (1996) , although no type genus was designated at that time, confirmed later by Stackebrandt et al (1997) , formally described by Normand (2006) and later emended by Zhi et al (2009) . The family accommodates the genera Blastococcus ( Ahrens and Moll, 1970 ; Skerman et al, 1980 ; Hezbri et al, 2016b ), Modestobacter ( Mevs et al, 2000 ) and the type genus Geodermatophilus ( Luedemann, 1968 ; Skerman et al, 1980 ).…”

Section: Introductionmentioning

confidence: 99%

Genome-Scale Data Call for a Taxonomic Rearrangement of Geodermatophilaceae

et al. 2017

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Geodermatophilaceae (order Geodermatophilales, class Actinobacteria) form a comparatively isolated family within the phylum Actinobacteria and harbor many strains adapted to extreme ecological niches and tolerant against reactive oxygen species. Clarifying the evolutionary history of Geodermatophilaceae was so far mainly hampered by the insufficient resolution of the main phylogenetic marker in use, the 16S rRNA gene. In conjunction with the taxonomic characterisation of a motile and aerobic strain, designated YIM M13156T and phylogenetically located within the family, we here carried out a phylogenetic analysis of the genome sequences now available for the type strains of Geodermatophilaceae and re-analyzed the previously assembled phenotypic data. The results indicated that the largest genus, Geodermatophilus, is not monophyletic, hence the arrangement of the genera of Geodermatophilaceae must be reconsidered. Taxonomic markers such as polar lipids and fatty-acids profile, cellular features and temperature ranges are indeed heterogeneous within Geodermatophilus. In contrast to previous studies, we also address which of these features can be interpreted as apomorphies of which taxon, according to the principles of phylogenetic systematics. We thus propose a novel genus, Klenkia, with the type species Klenkia marina sp. nov. and harboring four species formerly assigned to Geodermatophilus, G. brasiliensis, G. soli, G. taihuensis, and G. terrae. Emended descriptions of all species of Geodermatophilaceae are provided for which type-strain genome sequences are publicly available. Our study again demonstrates that the principles of phylogenetic systematics can and should guide the interpretation of both genomic and phenotypic data.

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“…The genus Blastococcus of the family Geodermatophilaceae [1] was first proposed by Ahrens and Moll [2] and then emended by Urzi et al [3], Lee [4] and Hezbri et al [5]. Phylogenetic analysis based on 16S rRNA gene sequences showed that the genus Blastococcus falls within the family Geodermatophilaceae, order Geodermatophiliales, class Actinobacteria [6].…”

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confidence: 99%

“…At the time of writing, there were six validly published species of the genus Blastococcus [2-5, 7, 8]. Some strains of this genus were isolated from marine biotopes [2,4,9] and also found as endophytes [7], but most of them were best known to be monument and stone dwellers [3,5,8,10,11]. The isolation of Blastococcus strains from harsh environments suggests an effective ecological adaptation.…”

mentioning

confidence: 99%

“…The composition of peptidoglycan hydrolysates (6M HCl, 100 C for 16 h) was analysed by TLC on cellulose plates as outlined by Schleifer and Kandler [30]. For fatty acid analysis, an optimized protocol for Blastococcus species was used [5], which consists of growing cells on PYGV agar plates held at 20 C for 15 days and harvesting from the same sector (the last quadrant streak). Analysis was conducted using the Microbial Identification System (MIDI System; Sherlock Version 6.1 (method TSBA40, ACTIN6 database) [31].…”

mentioning

confidence: 99%

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Blastococcus xanthinilyticus sp. nov., isolated from monument

Hezbri

Nouioui

Rohde

et al. 2018

International Journal of Systematic and Evolutionary Microbiology

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A novel, non-motile, coccoid, Gram-stain-positive actinobacterium, designated BMG 862, was isolated from a marble sample collected from the Bulla Regia monument, Northern Tunisia. Its taxonomic position was determined using a polyphasic approach. Results from chemotaxonomic analyses showed MK-9(H4), MK-8(H4) and MK-9(H2) as the predominant menaquinones. The major polar lipids comprised diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylinositol, glycophosphatidylinositol, hydroxy-phosphatidylethanolamine and three unidentified phospholipids. The fatty acids consisted of significant amounts (≥10 %) of iso-C16 : 0, C17 : 1ω8c, iso-C15 : 0 and C16 : 1ω7c. Phylogenetic analysis on the basis of 16S rRNA gene sequence comparisons showed that strain BMG 862 belongs to the genus Blastococcus, being most closely related to Blastococcus saxobsidens (=DSM 44509) (99.5 %) and Blastococcus capsensis (=DSM 46835=CECT 8876) (99.3 %). The genomic DNA G+C content of the organism was 74.7 mol%. Results of DNA-DNA hybridization and physiological tests allowed differentiation of strain BMG 862 from related species. The strain was also characterized by its ability to hydrolyse xanthine. On the basis of phenotypic and molecular characteristics, strain BMG 862 (=DSM 46842=CECT 8884) represents the type strain of a novel species of the genus Blastococcus, for which the name Blastococcus xanthinilyticus sp. nov. is proposed.

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Blastococcus capsensis sp. nov., isolated from an archaeological Roman pool and emended description of the genus Blastococcus, B. aggregatus, B. saxobsidens, B. jejuensis and B. endophyticus

Cited by 40 publications

References 48 publications

Does the human placenta delivered at term have a microbiota? Results of cultivation, quantitative real-time PCR, 16S rRNA gene sequencing, and metagenomics

Does the human placenta delivered at term have a microbiota? Results of cultivation, quantitative real-time PCR, 16S rRNA gene sequencing, and metagenomics

Genome-Scale Data Call for a Taxonomic Rearrangement of Geodermatophilaceae

Blastococcus xanthinilyticus sp. nov., isolated from monument

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