Blastocyst development rate impacts outcome in cryopreserved blastocyst transfer cycles

Levens, Eric D.; Whitcomb, Brian W.; Hennessy, Sasha; James, Amy; Yauger, Belinda J.; Larsen, F.W.

doi:10.1016/j.fertnstert.2007.10.029

Cited by 60 publications

(42 citation statements)

References 20 publications

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“…Comparable pregnancy and live birth rates were achieved in both groups. These results stand in contrast to an earlier, similar study by Levens et al in 2008 showing a higher implantation rate in day 5 blastocysts than in day 6 blastocysts transferred on the 6th day of progesterone, although in this study, live birth rates were also similar between groups [9].…”

Section: Introductioncontrasting

confidence: 99%

Developmentally delayed cleavage-stage embryos maintain comparable implantation rates in frozen embryo transfers

Burks

Buckbinder

Francis-Hernandez

et al. 2015

J Assist Reprod Genet

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Purpose In fresh IVF cycles, embryos reaching the eight-cell stage on day 3 of development are thought to have a higher chance of implantation than those reaching this stage on day 4. To determine whether this difference persists after cryopreservation, we compared pregnancy and implantation rates between frozen embryo transfer (FET) cycles using delayed cleavage-stage embryos (cryopreserved day 4) and normal cleavage-stage embryos (cryopreserved day 3). Methods Participants underwent FET between 2008 and 2012 using embryos cryopreserved on either day 3 (n=76) or day 4 (n=48), depending on the length of time needed to achieve the eight-cell stage. All embryos, regardless of day of cryopreservation, were thawed and transferred on the 4th day of vaginal progesterone following endometrial preparation with oral estradiol. Chi-square and Mann-Whitney U tests were used to compare patient demographics and cycle outcomes. Results More women in the day 4 group had diminished ovarian reserve (44 vs 16 %, p=0.003). Pregnancy outcomes in preceding fresh cycles were not different between the two groups. Pregnancy, implantation, and live birth rates following FET did not differ between the day 3 and day 4 groups. Conclusions This is the first study to address outcomes using day 3 versus day 4 cryopreserved embryos. Despite a higher prevalence of diminished ovarian reserve (DOR) in the day 4 group, delayed cleavage-stage embryos utilized in FET cycles performed as well as embryos growing at the normal rate, suggesting delayed embryo development does not affect embryo implantation as long as endometrial synchrony is maintained.

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Section: Introductioncontrasting

confidence: 99%

Developmentally delayed cleavage-stage embryos maintain comparable implantation rates in frozen embryo transfers

Burks

Buckbinder

Francis-Hernandez

et al. 2015

J Assist Reprod Genet

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“…Some people have reported that the anabiosis rate of D5 early blastocysts (87-91%) is higher than that of expanded blastocysts (55-71%), so early blastocysts are more suitable to vitrification (Vanderzwalmen et al 2002;Hiraoka et al 2004). Some people have believed that vitrified D5 expanded blastocysts have higher clinical pregnancy rates (Levens et al 2008). Furthermore, it is unclear whether the artificial shrinkage technology is suitable to both early and expanded blastocysts.…”

Section: Resultsmentioning

confidence: 99%

“…There has been considerable professional debate about which blastocyst has better development potential after vitrification. Some people have reported that early blastocysts are more suitable for cryopreservation (Vanderzwalmen et al 2002;Hiraoka et al 2004), whereas others believe that expanded blastocysts have a higher anabiosis rate after cryopreservation (Levens et al 2008). To reduce cryodamage, blastocoeles were collapsed using microneedle or laser drilling prior to vitrification, and this procedure significantly improved the anabiosis rate of blastocysts (Mukaida et al 2006;Cao et al 2014).…”

Section: Introductionmentioning

confidence: 99%

“…The vitrification damage on the blastocysts also exhibits apoptosis of inner cell mass caused by accumulation of cell debris in perivitelline space and blastocoeles (Borini et al 2006;Carambula et al 2009). While blastular anabiosis rates can evaluate thawed blastocysts in morphological respect, blastular expansion and hatching rates can evaluate developmental potential of thawed blastocysts, and apoptosis in blastocysts can evaluate damage degree of thawed blastocysts (Hiraoka et al 2004;Mukaida et al 2006;Levens et al 2008;Van Landuyt et al 2011). Therefore, in this study, the anabiosis rate, expansion rate, hatching rate, and apoptosis were used to observe the vitrified effects on blastocysts.…”

Section: Introductionmentioning

confidence: 99%

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Comparison of vitrified outcomes between human early blastocysts and expanded blastocysts

Song

Wang

Jin

et al. 2016

In Vitro Cell.Dev.Biol.-Animal

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We compared the vitrified outcomes between early and expanded blastocysts with or without laser drilling. The grade III embryos from the patients undergoing in vitro fertilization-embryo transfer (IVF-ET) in our reproductive center from September 2009 to February 2015 were incubated into early blastocysts and expanded blastocysts. The early blastocysts and expanded blastocysts were, respectively, divided into laser group (vitrification after laser drilling), non-laser group (direct vitrification), and control group (fresh non-vitrified blastocysts). After thawing, the blastular anabiosis rate, expansion rate, hatching rate, and apoptosis were observed in each group and then were compared amongst groups. This study indicated that the blastular expansion rate (all P < 0.01) and hatching rate (all P < 0.01) were significantly lower, but the blastular apoptosis (all P < 0.05) was significantly higher in both laser and non-laser groups than in the control group in the early blastocysts. In the expanded blastocysts, the blastular anabiosis rate was significantly higher in the laser group than in the non-laser group (P < 0.01), and the blastular expansion rate was significantly higher, but the blastular apoptosis was significantly lower in both laser group and control group than in the non-laser group (all P < 0.05). The blastular expansion rate (all P < 0.01) and hatching rate (all P < 0.01) were significantly higher, but the blastular apoptosis (all P < 0.05) was significantly lower in the expanded laser group than in both early laser and early non-laser groups. We conclude that vitrification for laser-drilling expanded blastocysts can achieve the best outcomes.

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“…Kenichiro et al found that no statistically significant differences were observed in the implantation and pregnancy rates between D5 and D6 blastocysts [4]. Some studies, however, indicated that the implantation rate of D5 blastocyst after being frozen and thawed was significantly higher than that in the D6-blastocyst group, whereas the clinical pregnancy rate of the D5 blastocyst was slightly higher than that in the D6 group, but with no statistically significant difference [7]. A recent meta-analysis included 15 articles of frozen-thawed blastocyst transfer, including 2512 freeze-thaw cycles of blastocyst transfer [3].…”

Section: Discussionmentioning

confidence: 99%

Comparison of Pregnancy Outcomes of High-Quality D5- and D6-Blastocyst Transfer in Hormone-Replacement Frozen-Thawed Cycles

Xing¹,

Cai²,

Sun³

et al. 2017

IJCM

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This study aimed to assess pregnancy outcomes after high-quality D5-and D6-blastocyst transfer in frozen cycles of in vitro fertilization and embryo transfer and to further evaluate whether there was a difference in blastocyst development potentials with different developmental speeds and in pregnancy outcomes. A retrospective analysis was conducted to analyze 247 frozen cycles in our center from September 2015 to July 2017, which were divided into two groups: a D5-FET group with 193 cycles of D5-blastocyst transfer, and a D6-FET group with 54 cycles of D6-blastocyst transfer. Hormone replacement method was utilized to prepare frozen-cycle endometria. Pregnancy outcomes were analyzed and compared between these two groups. The mean ages of the two groups were 31.45 ± 4.43 years and 31.98 ± 4.84 years, respectively, with no statistically significant differences (P > 0.05). The difference in the endometrial thickness during transfer was also not statistically significant. The implantation rate in the D5-FET group was 60.13%, significantly higher than that in the D6-FET group (31.58%, P < 0.05). The clinical pregnancy rate in the D5-FET group was 68.91%, significantly higher than that in the D6-FET group (40.74%, P < 0.05). No statistically significant differences were found in the abortion rate and ectopic pregnancy rate between the two groups. The implantation, biochemical pregnancy, and clinical pregnancy rates of the blastocyst D5 were all superior to those of the blastocyst D6. In clinics, therefore, D5-blastocyst transfer could be prioritized for embryo transfer.

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Blastocyst development rate impacts outcome in cryopreserved blastocyst transfer cycles

Cited by 60 publications

References 20 publications

Developmentally delayed cleavage-stage embryos maintain comparable implantation rates in frozen embryo transfers

Developmentally delayed cleavage-stage embryos maintain comparable implantation rates in frozen embryo transfers

Comparison of vitrified outcomes between human early blastocysts and expanded blastocysts

Comparison of Pregnancy Outcomes of High-Quality D5- and D6-Blastocyst Transfer in Hormone-Replacement Frozen-Thawed Cycles

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