Blastocyst-induced changes in the bovine endometrial transcriptome

Passaro, Claudia; Tutt, Desmond; Mathew, Daniel J.; Sánchez, José María; Browne, John A.; Boe-Hansen, G.; Fair, Trudee; Lonergan, P.

doi:10.1530/rep-18-0188

Cited by 51 publications

(46 citation statements)

References 56 publications

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“…Despite the molecular complexity underlying the critical processes that take place in the periconception and early preimplantation period, the success of in vitro reproductive techniques suggests that the requirements of the embryo can be met by a relative simple set of media, and that embryonic development, at least to the blastocyst stage, occurs independently of interaction with the female reproductive tract. While blastocyst stage embryos can induce changes in the endometrium (Sponchiado et al, 2017;Passaro et al, 2018Passaro et al, , 2019 as well as the uterine lumen metabolite composition (Sponchiado et al, 2019), mainly through embryo-derived interferon-tau (Passaro et al, 2019), whether this interaction plays an important role in embryo survival is questionable given the fact that embryos can be transferred to a virgin uterus as late as Day 16 and still establish a pregnancy (Betteridge et al, 1980). Nonetheless, it is becoming apparent that offspring health can be affected by the environmental conditions experienced during gamete maturation, embryo development and fetal growth (Hanson and Gluckman, 2014).…”

Section: Introductionmentioning

confidence: 99%

Effect of Exposure to Seminal Plasma Through Natural Mating in Cattle on Conceptus Length and Gene Expression

Mateo‐Otero

Sánchez

Recuero

et al. 2020

Front. Cell Dev. Biol.

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A growing body of evidence suggests that paternal factors have an impact on offspring development. These studies have been mainly carried out in mice, where seminal plasma (SP) has been shown to regulate endometrial gene expression and impact embryo development and subsequent offspring health. In cattle, infusion of SP into the uterus also induces changes in endometrial gene expression, however, evidence for an effect of SP on early embryo development is lacking. In addition, during natural mating, the bull ejaculates in the vagina; hence, it is not clear whether any SP reaches the uterus in this species. Thus, the aim of the present study was to determine whether SP exposure leads to improved early embryo survival and developmental rates in cattle. To this end, Day 7 in vitro produced blastocysts were transferred to heifers (12-15 per heifer) previously mated to vasectomized bulls (n = 13 heifers) or left unmated (n = 12 heifers; control). At Day 14, heifers were slaughtered, and conceptuses were recovered to assess size, morphology and expression of candidate genes involved in different developmental pathways. Additionally, CL volume at Day 7, and weight and volume of CL at Day 14 were recorded. No effect of SP on CL volume and weight not on conceptus recovery rate was observed. However, filamentous conceptuses recovered from SP-exposed heifers were longer in comparison to the control group and differed in expression of CALM1, CITED1, DLD, HNRNPDL, PTGS2, and TGFB3. In conclusion, data indicate that female exposure to SP during natural mating can affect conceptus development in cattle. This is probably achieved through modulation of the female reproductive environment at the time of mating.

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Section: Introductionmentioning

confidence: 99%

Effect of Exposure to Seminal Plasma Through Natural Mating in Cattle on Conceptus Length and Gene Expression

Mateo‐Otero

Sánchez

Recuero

et al. 2020

Front. Cell Dev. Biol.

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“…Previously, the presence of multiple Day-7 embryo has been reported to amplify the embryo-derived signals in the uterus5,19 and thus, this experimental model detected amplified signals. P4 concentration at Day-1 was served as control.…”

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confidence: 65%

“…7,8 It is produced from trophectoderm of the bovine embryo and secreted into the uterus, in which it activates interferon-stimulated genes (ISGs) as early as at Day-7 of pregnancy in cows. 9 Interestingly, expression of ISGs has also been found in extra-uterine tissues such as in corpus luteum 10 and in peripheral blood immune cells [11][12][13][14] during blastocyst development and pre-hatching period (Day-8), maternal recognition of pregnancy (Day- 16), and peri-implantation periods (Day [18][19] in cows, suggesting that IFNT could be involved in modulation of peripheral immune response for tolerance of embryos in the uterus during very early stages of pregnancy. However, whether Day-7 embryos in the uterus regulate the immune-related gene expression of peripheral blood immune cells is still obscure.…”

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confidence: 99%

Day‐7 embryos generate an anti‐inflammatory immune response in peripheral blood immune cells in superovulated cows

Talukder

Rashid

Takedomi³

et al. 2018

American J Rep Immunol

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Problem Recently, we demonstrated both in vitro and in vivo that an immunological crosstalk between Day‐7 embryo and immune cells exists locally in the uterus in cows. The peripheral immune response to early embryos at Day‐7 of pregnancy in cows remains largely unknown. Thus, we aimed to investigate the response of peripheral blood immune cells in the presence of multiple Day‐7 embryos in the uterus in donor cows of embryo transfer program. Method of study Superovulated cows were either inseminated (n = 13) at 12‐18 hours post‐estrus or remained without inseminations (n = 6). Blood was collected following insemination (Day‐1) and immediately after uterine flushing (Day‐7). Polymorphonuclear neutrophils (PMNs) and peripheral blood mononuclear cells (PBMCs) were isolated from whole blood for analysis of gene expression. Results Interferon‐stimulated genes (ISGs: ISG15 and OAS1) were increased in both PMNs and PBMCs, with up‐regulation of PTGES and anti‐inflammatory cytokines (TGFB1 and IL10) expressions at Day‐7 of post‐inseminations in cows, when compared to those at Day‐7 in non‐inseminated cows. Conclusion The findings indicate that the presence of multiple embryos in the uterus generates an anti‐inflammatory immune response in peripheral blood immune cells at Day‐7 of pregnancy in cows, which might help to accept semi‐allogenic embryo in the uterus.

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“…This indicates that interaction with the female reproductive tract, while not strictly necessary for early embryo development, enhances embryo quality [4]. More recently, local effects of the embryo on the endometrium at the blastocyst stage have been demonstrated [5][6][7]. Following hatching of the blastocyst from the zona pellucida, there begins a sequential modification to the transcriptome of the endometrium as early as Day 13 [8,9], which is amplified during the time of pregnancy recognition (Day 16) [10][11][12] This response is further propagated into extrauterine tissues, including the corpus luteum (CL) and peripheral blood [13][14][15][16].…”

Section: Introductionmentioning

confidence: 99%

Protein Synthesis by Day 16 Bovine Conceptuses during the Time of Maternal Recognition of Pregnancy

Estepa

Tinning

Vasconcelos

et al. 2020

IJMS

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Interferon Tau (IFNT), the conceptus-derived pregnancy recognition signal in cattle, significantly modifies the transcriptome of the endometrium. However, the endometrium also responds to IFNT-independent conceptus-derived products. The aim of this study was to determine what proteins are produced by the bovine conceptus that may facilitate the pregnancy recognition process in cattle. We analysed by mass spectrometry the proteins present in conceptus-conditioned media (CCM) after 6 h culture of Day 16 bovine conceptuses (n = 8) in SILAC media (arginine- and lysine-depleted media supplemented with heavy isotopes) and the protein content of extracellular vesicles (EVs) isolated from uterine luminal fluid (ULF) of Day 16 pregnant (n = 7) and cyclic (n = 6) cross-bred heifers on day 16. In total, 11,122 proteins were identified in the CCM. Of these, 5.95% (662) had peptides with heavy labelled amino acids, i.e., de novo synthesised by the conceptuses. None of these proteins were detected in the EVs isolated from ULF. Pregnancy-associated glycoprotein 11, Trophoblast Kunitz domain protein 1 and DExD-Box Helicase 39A were de novo produced and present in the CCM from all conceptuses and in previously published CCM data following 6 and 24 h. A total of 463 proteins were present in the CCM from all the conceptuses in the present study, and after 6 and 24 h culture in a previous study, while expression of their transcripts was not detected in endometrium indicating that they are likely conceptus-derived. Of the proteins present in the EVs, 67 were uniquely identified in ULF from pregnant heifers; 35 of these had been previously reported in CCM from Day 16 conceptuses. This study has narrowed a set of conceptus-derived proteins that may be involved in EV-mediated IFNT-independent embryo–maternal communication during pregnancy recognition in cattle.

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Blastocyst-induced changes in the bovine endometrial transcriptome

Cited by 51 publications

References 56 publications

Effect of Exposure to Seminal Plasma Through Natural Mating in Cattle on Conceptus Length and Gene Expression

Effect of Exposure to Seminal Plasma Through Natural Mating in Cattle on Conceptus Length and Gene Expression

Day‐7 embryos generate an anti‐inflammatory immune response in peripheral blood immune cells in superovulated cows

Protein Synthesis by Day 16 Bovine Conceptuses during the Time of Maternal Recognition of Pregnancy

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