Blastocyst morphology, actin cytoskeleton quality and chromosome content are correlated with embryo quality in the pig

Zijlstra, C.; Kidson, A; Schoevers, E.J.; Daemen, A.J.J.M.; Tharasanit, Theerawat; Kuijk, Ewart; Hazeleger, W.; Ducro-Steverink, D.W.B.; Colenbrander, B.

doi:10.1016/j.theriogenology.2008.05.055

Cited by 18 publications

(17 citation statements)

References 31 publications

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“…It was found that in in vivo pig blastocysts the ploidy mosaicism is restricted to only a minority of cells (approx. 5%) [21], and our present study supports that observation.…”

Section: Discussionsupporting

confidence: 93%

Aneuploidy Detection in Pigs Using Comparative Genomic Hybridization: From the Oocytes to Blastocysts

et al. 2012

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Data on the frequency of aneuploidy in farm animals are lacking and there is the need for a reliable technique which is capable of detecting all chromosomes simultaneously in a single cell. With the employment of comparative genomic hybridization coupled with the whole genome amplification technique, this study brings new information regarding the aneuploidy of individual chromosomes in pigs. Focus is directed on in vivo porcine blastocysts and late morulas, 4.7% of which were found to carry chromosomal abnormality. Further, ploidy abnormalities were examined using FISH in a sample of porcine embryos. True polyploidy was relatively rare (1.6%), whilst mixoploidy was presented in 46.8% of embryos, however it was restricted to only a small number of cells per embryo. The combined data indicates that aneuploidy is not a prevalent cause of embryo mortality in pigs.

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“…It was found that in in vivo pig blastocysts the ploidy mosaicism is restricted to only a minority of cells (approx. 5%) [21], and our present study supports that observation.…”

Section: Discussionsupporting

confidence: 93%

Aneuploidy Detection in Pigs Using Comparative Genomic Hybridization: From the Oocytes to Blastocysts

et al. 2012

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“…These criteria were adapted from previous studies [33][34][35]. Blastocysts were classified into grades I to III according to the integrity of their actin cytoskeleton, as previously described [28,36,37]. Grade I was characterized by distinct actin staining in the blastomeres borders with an abundant and even microfilament complement in the cytoplasm (Fig.…”

Section: Classification Of Actin Stainingmentioning

confidence: 99%

“…The polymerization of non-filamentous actin into microfilaments (F-actin) is important for both porcine meiosis and mitosis (i.e., for oocyte maturation and early embryo development) [27]. In addition, actin cytoskeleton integrity was previously related with embryo quality [28].…”

Section: Introductionmentioning

confidence: 99%

Effect of blood plasma collected after adrenocorticotropic hormone administration during the preovulatory period in the sow on oocyte in vitro maturation

González

2013

Theriogenology

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Reproduction may be affected by stressful events changing the female endocrine or metabolic profile. An altered environment during oocyte development could influence the delicate process of oocyte maturation. Here, the effect of simulated stress by media supplementation with blood plasma from sows after adrenocorticotropic hormone (ACTH) administration during the preovulatory period was assessed. Oocytes were matured for 46 h in the presence of plasma from ACTH-treated sows, or plasma from NaCl-treated control sows, or medium without plasma (BSA group). The plasma used had been collected at 36 h and 12 h (± 2 h) before ovulation (for the first 24 h + last 22 h of maturation, respectively).Subsequent fertilisation and embryo development were evaluated. Actin cytoskeleton and mitochondrial patterns were studied by confocal microscopy both in the oocytes and the resulting blastocysts. Nuclear maturation did not differ between treatments. Subtle differences were observed in the actin microfilaments in oocytes; however, mitochondrial patterns were associated with the treatment (P<0.001). These differences in mitochondrial patterns were not reflected by in vitro outcomes, which were similar in all groups. In conclusion, an altered hormonal environment provided by a brief exposure to plasma from ACTH-treated sows during in vitro oocyte maturation, could induce alterations in actin cytoskeleton and mitochondrial patterns in oocytes. However, these changes might not hamper the subsequent in vitro embryo development.[217 words]

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“…In the Control group, more than half of the injected oocytes were arrested at the metaphase-II (M-II) stage but most of the injected sperm nuclei underwent decondensation spontaneously triggered by ooplasmic glutathione [12]. A rise in the intracellular level of free Ca 2+ is not necessary for the decondensation [13], whereas transition of the decondensed sperm nucleus to the male PN does require this either [4]. These results suggest that a decrease of oocyte-activating ability of the sperm, possibly attributable to the quantity of PLCζ present in the sperm, also leads to failure of male PN formation.…”

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confidence: 99%

“…The rate of blastocyst formation in the Percoll and Cont + ES groups was significantly higher than that in the Control group (P < 0.05). In contrast, the mean number of cells per blastocyst, which is an important indicator of embryo quality [13], after electrical stimulation was lower in both, the Percoll and Control groups (Table 2). It has been reported that the pattern of Ca 2+ oscillation induced by electrical stimulation differs from that observed in the sperm-penetrated oocytes [15].…”

mentioning

confidence: 99%

Efficient pig ICSI using Percoll-selected spermatozoa; evidence for the essential role of phospholipase C-ζ in ICSI success

Nakai

Suzuki

Ito

et al. 2016

Journal of Reproduction and Development

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In pigs, the damaged sperm membrane leads to leakage of phospholipase C-ζ (PLCζ), which has been identified as a sperm factor, and a reduction of oocyte-activating ability. In this study, we investigated whether sperm selected by Percoll gradient centrifugation (Percoll) have sufficient PLCζ, and whether the efficiency of fertilization and blastocyst formation after intracytoplasmic sperm injection (ICSI) using Percoll-selected sperm can be improved. Percoll-selected sperm (Percoll group) or sperm without Percoll selection (Control group) were used. A proportion of the oocytes injected with control sperm were subjected to electrical stimulation at 1 h after ICSI (Cont + ES group). It was found that the Percoll group showed a large amount of PLCζ in comparison with the Control group. Furthermore, application of Percoll-selected sperm for ICSI increased the efficiency of fertilization and embryo development. Thus, these results indicate the Percoll-selected sperm have sufficient PLCζ and high oocyte-activating ability after ICSI in pigs.

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Blastocyst morphology, actin cytoskeleton quality and chromosome content are correlated with embryo quality in the pig

Cited by 18 publications

References 31 publications

Aneuploidy Detection in Pigs Using Comparative Genomic Hybridization: From the Oocytes to Blastocysts

Aneuploidy Detection in Pigs Using Comparative Genomic Hybridization: From the Oocytes to Blastocysts

Effect of blood plasma collected after adrenocorticotropic hormone administration during the preovulatory period in the sow on oocyte in vitro maturation

Efficient pig ICSI using Percoll-selected spermatozoa; evidence for the essential role of phospholipase C-ζ in ICSI success

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