Blastogenesis and polyclonal immunoglobulin synthesis in murine spleen cells stimulated with lipoolysaccharide, lipid A and acid-degrade polysaccharide from Fusobacterium nucleatum

Comparative mitogenicity and polyclonal B cell activation capacity of eight oral or nonoral bacterial lipopolysaccharides in cultures of spleen cells from athymic (nu/nu‐BALB/c) and thymic (BALB/c) mice

“…The results of the kinetic studies strongly indicated simultaneous response of IgM-and IgA-producing B cell subsets rather than a switch from primarily an IgM to a subsequent IgA production. Bimodal LPS-stimulated Ig profiles in vitro have also been reported by others (1,29,37) and have been explained by an Ig feedback mechanism giving a decline in Ig production after the Ig peaks have been reached (29). Within the limitations of our study, there is no other plausible explanations for the observed range in optimal LPS polyclonal Ig-stimulatory doses than those given above to explain variations in optimal mitogenic stimulatory doses of LPS.…”

“…Later it has been shown in vitro that purified polysaccharide derived from phenol-water extracted LPS of B. fragUis (41) and of E nucleatum (37) stimulated blastogenesis and Ig synthesis in murine spleen cell cultures. Acid-degraded polysaccharide from LPS of Wolinella reeta (17) and E tiueleatum (37) have proved to be a rnuch weaker mitogen than the complete LPS. We found that B. fragdis LPS was a more potent B cell stimulant than was LPS containing 3-hydroxyrnyristic acid.…”

Virulence factors in anaerobic bacteri

“…Fusobacterium nucleatum has a conventional endotoxic LPS. It has recently been shown that blastogenesis and polyclonal immunoglobulin synthesis in murine spleen cells are stimulated not only by the LPS and lipid A of Fusobacterium nucleatum but also by the LPS polysaccharide component as obtained by mild acid hydrolysis (49), and that interchangeable combinations of LPS, lipid A and degraded polysaccharide have a synergistic effect (50).…”