Blautia intestinalis sp. nov., isolated from human feces

Wang, Yujing; Abdugheni, Rashidin; Liu, Chang; Zhou, Nan; You, Xin; Liu, Shuang‐Jiang

doi:10.1099/ijsem.0.005005

Cited by 9 publications

(2 citation statements)

References 33 publications

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“…Therefore, these increasing taxa might mainly contribute to GbE-mediated SCFAs generation. However, the Blautia and Roseburia genera, which contain SCFA-producing species (Wang et al, 2021;Yao et al, 2020), were negatively correlated with SCFAs levels in our study. We hypothesized that GbE treatment might influence other species subordinated to Blautia and Roseburia except the SCFA-producing ones.…”

Section: Fmt Ameliorates the Wd-induced Atherosclerosis And Hyperchol...contrasting

confidence: 68%

Ginkgo biloba extract ameliorates atherosclerosis via rebalancing gut flora and microbial metabolism

Wang

et al. 2022

Phytotherapy Research

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The Ginkgo biloba leave extract (GbE) is widely applied in the prevention and treatment of atherosclerotic cardiovascular diseases in clinical practice. However, its mechanism of actions has not been totally elucidated. In this study, we confirmed the beneficial effects of GbE in alleviating hypercholesterolemia, inflammation and atherosclerosis in Ldlr À/À mice, which were fed 12 weeks of Western diet (WD). Moreover, 16S rRNA sequencing revealed that GbE treatment reshaped the WDperturbed intestinal microbiota, particularly decreased the Firmicutes/Bacteroidetes ratio and elevated the abundance of Akkermansia, Alloprevotella, Alistipes, and Parabacteroides. Furthermore, GbE treatment downregulated the intestinal transcriptional levels of proinflammatory cytokines and enhanced the expression of tight junction proteins, exerting the roles of attenuating the intestinal inflammation as well as repairing the gut barrier. Meanwhile, the targeted metabolomic analysis displayed that GbE treatment significantly reversed the dysfunction of the microbial metabolic phenotypes, including promoting the production of short chain fatty acids, indole-3-acetate and secondary bile acids, which were correlated with the atherosclerotic plaque areas. Finally, we confirmed GbE-altered gut microbiota was sufficient to alleviate atherosclerosis by fecal microbiota transplantation. In summary, our findings provide important insights into the pharmacological mechanism underlying the antiatherogenic efficacy of GbE.

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Section: Fmt Ameliorates the Wd-induced Atherosclerosis And Hyperchol...contrasting

confidence: 68%

Ginkgo biloba extract ameliorates atherosclerosis via rebalancing gut flora and microbial metabolism

Wang

et al. 2022

Phytotherapy Research

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“…SCFAs were analysed with a culture of strain NSJ-9 T grown on M2GSC medium at 37 °C for 72 h according to our previous method [50]. The whole-cell fatty acids were extracted, saponified and methylated according to the standard protocol of the Sherlock Microbial Identification System version 6.0 (MIDI), analysed by GC (HP 6890 Series GC System; Agilent) and identified using the TSBA6 database of the Microbial Identification System.…”

Section: Full-textmentioning

confidence: 99%

Pararoseburia lenta gen. nov., sp. nov. isolated from human faeces

Abdugheni

Wang

et al. 2022

International Journal of Systematic and Evolutionary Microbiology

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A strictly anaerobic, motile bacterium, designated as strain NSJ-9T, was isolated from human faeces. Cells were Gram-negative, non-spore-forming, non-pigmented, and spiral-shaped or slightly curved rods with flagella. Optimal growth in M2GSC medium was observed at 37 °C (growth range 30–45 °C) and pH 6.5–7.0 (growth range 6.5–7.5) under anaerobic conditions. Phylogenetic analysis of the 16S rRNA gene revealed that strain NSJ-9T formed a distinct phylogenetic lineage that reflects a new genus in the family Lachnospiraceae , with high levels of similarity to Roseburia hominis A2-183T (95.2 %), Roseburia cecicola ATCC 33874T (95.2 %), Pseudobutyrivibrio ruminis DSM 9787T (95.2 %), Pseudobutyrivibrio xylanivorans MZ 5T (94.8%) and Roseburia faecis M72/1T (94.4 %). Genomic similarity (average nucleotide identity and digital DNA–DNA hybridization) values between strain NSJ-9T and its phylogenetic neighbours were below 71 and 31 %, respectively, indicating that strain NSJ-9T represented a novel species. The average amino acid identity and the percentage of conserved proteins between strain NSJ-9T and other related members of the family Lachnospiraceae were below 63 and 50 %, respectively, supporting that strain NSJ-9T was a member of a new genus. The predominant cellular fatty acids of strain NSJ-9T were C16 : 0 and C17 : 0 2-OH, and major polar lipids were glycolipids. The end products of glucose fermentation were acetate, propionate, iso-butyrate, butyrate and valerate. Phylogenetic and phylogenomic lineage, pairwise determined genome identity analysis suggested that strain NSJ-9T represents a novel genus in the family Lachnospiraceae . The genome size of strain NSJ-9T is 2.56 Mbp with 44.9 mol% G+C content. Collectively, the genotypic and phenotypic differences between phylogenetic relatives suggested strain NSJ-9T represented a novel species of a new genus, for which the name Pararoseburia lenta gen. nov., sp. nov. is proposed. The type strain of Pararoseburia lenta is NSJ-9T (=CGMCC 1.32469T=KCTC 15957T).

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Update on Accepted Novel Bacterial Isolates Derived from Human Clinical Specimens and Taxonomic Revisions Published in 2020 and 2021

Munson

Carroll

2023

J Clin Microbiol

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A number of factors, including microbiome analyses and the increased utilization of whole-genome sequencing in the clinical microbiology laboratory, has contributed to the explosion of novel prokaryotic species discovery, as well as bacterial taxonomy revision. This review attempts to summarize such changes relative to human clinical specimens that occurred in 2020 and 2021, per primary publication in the International Journal of Systematic and Evolutionary Microbiology or acceptance on Validation Lists published by the International Journal of Systematic and Evolutionary Microbiology .

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Blautia intestinalis sp. nov., isolated from human feces

Cited by 9 publications

References 33 publications

Ginkgo biloba extract ameliorates atherosclerosis via rebalancing gut flora and microbial metabolism

Ginkgo biloba extract ameliorates atherosclerosis via rebalancing gut flora and microbial metabolism

Pararoseburia lenta gen. nov., sp. nov. isolated from human faeces

Update on Accepted Novel Bacterial Isolates Derived from Human Clinical Specimens and Taxonomic Revisions Published in 2020 and 2021

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