2019
DOI: 10.1364/boe.10.001446
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Bleaching effects and fluorescence lifetime imaging ophthalmoscopy

Abstract: This study investigates the influence of photopigment bleaching on autofluorescence lifetimes in the fundus in 21 young healthy volunteers. Three measurements of 30° retinal fields in two spectral channels (SSC: 498-560 nm, LSC: 560-720 nm) were obtained for each volunteer using fluorescence lifetime imaging ophthalmoscopy (FLIO). After dark-adaptation by wearing a custom-made lightproof mask for 30 minutes, the first FLIO-measurement was recorded (dark-adapted state). Subsequently, the eye was bleached for 1 … Show more

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Cited by 8 publications
(4 citation statements)
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References 56 publications
(62 reference statements)
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“…Previous important imaging studies have proved that photobleaching exposure may be associated with significant changes. In detail, fundus autofluorescence studies demonstrated that photobleaching may cause an immediate reduction in signal intensity 1922. In addition, previous important studies employing autofluorescence also assessed diseased eyes, which further improve our understanding on this phenomenon 23,24.…”
Section: Introductionmentioning
confidence: 92%
“…Previous important imaging studies have proved that photobleaching exposure may be associated with significant changes. In detail, fundus autofluorescence studies demonstrated that photobleaching may cause an immediate reduction in signal intensity 1922. In addition, previous important studies employing autofluorescence also assessed diseased eyes, which further improve our understanding on this phenomenon 23,24.…”
Section: Introductionmentioning
confidence: 92%
“…One recently added and popular option for RT-FLIM for laser-scanning microscopy is the commercially available closed-source Leica FALCON system (Alvarez et al, 2019). Other FLIM hardware vendors such as Becker-Hickl, PicoQuant, and ISS have their own applications that support a version of real-time FLIM (Liao et al, 2014;Orthaus-Mueller et al, 2016;Becker, 2021) and custom laboratory-made applications (Laptenok et al, 2007;Klemm et al, 2015;Zhang et al, 2021). Unfortunately, there are currently no open-source options for RT-FLIM, severely limiting access and also preventing code iteration by other FLIM laboratories.…”
Section: Introductionmentioning
confidence: 99%
“… 3 The potential of FLIM was reflected in studying the metabolic activities, 4 the drug delivery and release, 5 the basic mechanisms of diseases, 6 and medical diagnostics. 7 9 …”
Section: Introductionmentioning
confidence: 99%
“…3 The potential of FLIM was reflected in studying the metabolic activities, 4 the drug delivery and release, 5 the basic mechanisms of diseases, 6 and medical diagnostics. [7][8][9] In practice, the measurement of fluorescence lifetime can be achieved in frequency or time domain. [10][11][12] The frequency-domain FLIM uses modulated sinusoidal or pulsed excitation and measures the changes of the modulation depth and phase of the fluorescence emission with respect to the excitation signal.…”
Section: Introductionmentioning
confidence: 99%