Blebbing dynamics during endothelial cell spreading

Norman, Leann L.; Sengupta, Kheya; Aranda‐Espinoza, Helim

doi:10.1016/j.ejcb.2010.09.013

Cited by 38 publications

(40 citation statements)

References 54 publications

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“…In contrast, efficient blebbing was not observed at any time of the observation period when cells were plated on Fbn-coated surfaces ( Figure 2B, left panel). Consistent with a recent report [30], cell spreading, however, steadily increased with time on Fbn-coated surfaces, while blebbing cells on uncoated surfaces only displayed inefficient cell spreading ( Figure 2B, right panel). To test if this switch between PM blebbing and cell spreading in the presence of Fbn was mediated by integrins, cells were incubated with the β1-integrin-blocking antibody 4B4 or with an antibody against transferrin receptor (TfR) as isotype control.…”

Section: Inverse Correlation Between Pm Blebbing and Cell Spreadingsupporting

confidence: 80%

Differing and isoform-specific roles for the formin DIAPH3 in plasma membrane blebbing and filopodia formation

et al. 2011

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Plasma membrane (PM) blebs are dynamic actin-rich cell protrusions that occur, e.g., during cytokinesis, amoeboid cell motility and cell attachment. Using a targeted siRNA screen against 21 actin nucleation factors, we identify a novel and essential role of the human diaphanous formin DIAPH3 in PM blebbing during cell adhesion. Suppression of DIAPH3 inhibited blebbing to promote rapid cell spreading involving β1-integrin. Multiple isoforms of DIAPH3 were detected on the mRNA and protein level of which isoforms 3 and 7 were the largest and most abundant isoforms that however did not induce formation of actin-rich protrusions. Rather, PM blebbing specifically involved the low abundance isoform 1 of DIAPH3 and activation of isoform 7 by deletion of the diaphanous-autoregulatory domain caused the formation of filopodia. Dimerization and actin assembly activity were essential for induction of specific cell protrusions by DIAPH3 isoforms 1 and 7. Our data suggest that the N-terminal region comprising the GTPasebinding domain determined the subcellular localization of the formin as well as its protrusion activity between blebs and filopodia. We propose that isoform-selective actin assembly by DIAPH3 exerts specific and differentially regulated functions during cell adhesion and motility.

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Section: Inverse Correlation Between Pm Blebbing and Cell Spreadingsupporting

confidence: 80%

Differing and isoform-specific roles for the formin DIAPH3 in plasma membrane blebbing and filopodia formation

et al. 2011

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“…2C), and together with results from CHO ␣IIb␤3 cells and platelets (Fig. 2, A and B), these data suggest that integrin-ligand interactions induce cell adhesion to the solid substrate, and that membrane blebbing is a common phenomenon that occurs in the early stages of cell spreading (4).…”

Section: Discussionmentioning

confidence: 85%

“…Our results demonstrate that both NHE1 and NCX1 play roles in maintaining membrane blebbing, which is involved in cell migration (1)(2)(3)(4). There are forms of integrin-dependent metastatic cancer (34), and we speculate that up-regulation of NHE1 and NCX1 might occur in these cells.…”

Section: Discussionmentioning

confidence: 94%

“…The blebs expand (membrane bleb growth) by transient increases in intracellular pressure accompanied by reassembly of F-actin in the bleb cortex by an unknown mechanism, which then acts as a track for sliding of myosin motors (membrane bleb retraction) (3). Membrane blebbing is associated with many cellular activities, such as cell adhesion, spreading, migration, virus entry, cytokinesis, and apoptosis (1)(2)(3)(4). Although integrin receptors, which are heterodimers formed by combination of 18 ␣ and 8 ␤ subunits, and their extracellular matrix ligand proteins, either collagen, laminin, or fibronectin, also regulate many of these cellular activities (5,6), it is unclear whether integrin-mediated signals are involved in membrane blebbing.…”

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confidence: 99%

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Integrin-mediated Membrane Blebbing Is Dependent on Sodium-Proton Exchanger 1 and Sodium-Calcium Exchanger 1 Activity

Chang

Lin³

et al. 2012

Journal of Biological Chemistry

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Background: Integrin signaling and membrane blebbing modulate cell adhesion and migration, however, the link between them is unknown. Results: NHE1 and NCX1 are located in the bleb membrane, where they modulate integrin-mediated membrane blebbing. Conclusion: NHE1 and NCX1 functional coupling is important for bleb growth and retraction. Significance: These data reveal novel functionality for NHE1 and NCX1 in both integrin signaling and membrane blebbing.

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“…Speeds on the order of 3 nm∕s are associated with cell shape changes as cells respond to their force environment. [54][55][56][57] Speeds on the order of 30 nm∕s are associated with nuclear motions and membrane dynamics. the order of 300 nm∕s and above are associated with organelle transport and cytoskeletal restructuring.…”

Section: Biodynamic Imagingmentioning

confidence: 99%

Biodynamic imaging for phenotypic profiling of three-dimensional tissue culture

Sun

Merrill

An³

et al. 2017

J. Biomed. Opt

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Abstract. Three-dimensional (3-D) tissue culture represents a more biologically relevant environment for testing new drugs compared to conventional two-dimensional cancer cell culture models. Biodynamic imaging is a highcontent 3-D optical imaging technology based on low-coherence interferometry and digital holography that uses dynamic speckle as high-content image contrast to probe deep inside 3-D tissue. Speckle contrast is shown to be a scaling function of the acquisition time relative to the persistence time of intracellular transport and hence provides a measure of cellular activity. Cellular responses of 3-D multicellular spheroids to paclitaxel are compared among three different growth techniques: rotating bioreactor (BR), hanging-drop (HD), and nonadherent (U-bottom, UB) plate spheroids, compared with ex vivo living tissues. HD spheroids have the most homogeneous tissue, whereas BR spheroids display large sample-to-sample variability as well as spatial heterogeneity. The responses of BR-grown tumor spheroids to paclitaxel are more similar to those of ex vivo biopsies than the responses of spheroids grown using HD or plate methods. The rate of mitosis inhibition by application of taxol is measured through tissue dynamics spectroscopic imaging, demonstrating the ability to monitor antimitotic chemotherapy. These results illustrate the potential use of low-coherence digital holography for 3-D pharmaceutical screening applications.

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Blebbing dynamics during endothelial cell spreading

Cited by 38 publications

References 54 publications

Differing and isoform-specific roles for the formin DIAPH3 in plasma membrane blebbing and filopodia formation

Differing and isoform-specific roles for the formin DIAPH3 in plasma membrane blebbing and filopodia formation

Integrin-mediated Membrane Blebbing Is Dependent on Sodium-Proton Exchanger 1 and Sodium-Calcium Exchanger 1 Activity

Biodynamic imaging for phenotypic profiling of three-dimensional tissue culture

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