The paper's research focuses on the development of novel hydrophobizing agents and the demonstration of their efficiency on a concrete surface. The starting materials are based on natural oils, water-soluble silanes and siloxanes, and/or tap water. Aqueous preparations have qualities that limit the influence of volatile organic compounds (VOCs) on the environment since they solely include biodegradable components. According to research, the higher fatty acids obtained from vegetable oils and derived from the glycerin phase can be employed as a component of concrete hydrophobizing agents while matching the standard requirements for such agents. Analysis of the usage of vegetable oil as a basis ingredient in the manufacturing of industrial substances shows that it is biodegradable with at least 95% certainty. The reference concrete after the frost resistance test showed a weight loss of 1.8%. In the case of hydrophobization, the weight loss was 33.3 to 72% less than the reference samples. In the case of concrete hydrophobized with the glycerin agents, the salt crystallization occurred inside the structure of the material without causing any damage, with the reference concrete showing a weight loss of 0.8%.