bli-4

Bruchez, J. J. P.; Eberle, J.; Kohler, W.; Kruft, V.; Radford, A.; Russo, V. E. A.

doi:10.1007/s004380050223

Cited by 2 publications

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“…WC-1 also has one LOV domain, which is a putative chromophore-binding site. The WC-1⅐WC-2 complex is reported to bind FAD (flavin-adenine dinucleotide) (20).Blue light-responsive albino genes, al-1, al-2, and al-3, which encode enzymes for the synthesis of carotenoids (21-23), conidiation (con) 10 related to photo-conidiation (24) and blue light inducible genes bli-4 and bli-6 are transcriptionally activated after blue illumination of mycelia (25). No light-induced accumulation in al-1, al-2, al-3, or con-10 transcripts in the mycelia was observed in either wc-1 or wc-2 mutants (21,23,24,26).…”

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Reactive Oxygen Species Affect Photomorphogenesis in Neurospora crassa

Yoshida

Hasunuma

2004

Journal of Biological Chemistry

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In Neurospora crassa, several biological phenomena such as the synthesis of carotenoids in the mycelia and polarity of perithecia are regulated by light. We found that a sod-1 mutant, with a defective Cu,Zn-type superoxide dismutase (SOD), showed accelerated lightdependent induction of carotenoid accumulation in the mycelia compared with the wild type. The initial rate of light-induced carotenoid accumulation in the sod-1 mutant was faster than that in the vvd mutant known to accumulate high concentrations. This acceleration was suppressed by treatment with antioxidant reagents. Light-induced transcription of genes involved in carotenoid synthesis, al-1, -2, and -3, was sustained in the sod-1 mutant, whereas it was transient in the wild type. Moreover sod-1 was defective in terms of light-induced polarity of perithecia. By genetic analysis, the enhancement in light-inducible carotenoid synthesis in sod-1 was dependent on the wild type alleles of wc-1 and wc-2. However, the sod-1;vvd double mutant showed additive effects on the carotenoid accumulation in the mycelia. These results suggested that intracellular reactive oxygen species regulated by SOD-1 could affect the lightsignal transduction pathway via WC proteins.Light is one of the most important environmental factors being utilized for various biological phenomena by living organisms (1, 2). Typical examples are the visual systems in animals, photosynthesis in plants, and photomorphogenesis in fungi. In Neurospora crassa, several biological phenomena are under the control of blue light. Light-induced processes are summarized as follows: (i) synthesis of carotenoids in the mycelia (3), (ii) induction of conidiation (3), (iii) formation of protoperithecia on nitrogen-deficient media (4), (vi) positive phototropism of perithecial beaks (5), (v) positioning of the beak formed on the perithecium (perithecial polarity) (6, 7), and (vi) phase shift in the circadian rhythm of conidiation (8, 9).Much information on photoreceptors and light-inducible genes has been accumulated by genetic and molecular biological analyses to understand the molecular mechanism of these photobiological phenomena. In the search for the blue light photoreceptor in N. crassa, light-insensitive mutants, white collar (wc)1 -1 and wc-2, deficient in most of these blue light responses, have been isolated (4, 10, 11). The products for these genes are photoreceptors that can activate transcription. Both possess a PAS (PER, ARNT, and SIM) domain, a zinc finger DNA binding motif, and a glutamine-rich putative transcription activation domain (12, 13). WC proteins form heterodimers presumably through their PAS domains (14, 15), having promoter binding activity of blue light-regulated genes in Neurospora crassa.In Arabidopsis thaliana, three major groups of photoreceptors, phytochromes (16), cryptochromes (17), and phototropins (18), have been identified. Phototropins have two LOV (light, oxygen, and voltage) domains, and bind FMN (flavin mononucleotide) as a chromophore (19). WC-1 also has one LOV do...

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Reactive Oxygen Species Affect Photomorphogenesis in Neurospora crassa

Yoshida

Hasunuma

2004

Journal of Biological Chemistry

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Omics driven onboarding of the carotenoid producing red yeastXanthophyllomyces dendrorhousCBS 6938

Tobin

Collins

Marsan

et al. 2023

Preprint

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Genomics has become the primary way to explore microbial diversity, because genetic tools are currently difficult to develop in non-model organisms. Here, we demonstrate that -omics can be leveraged to accelerate genetic tool development for the basidiomycete yeastXanthophyllomyces dendrorhousCBS 6938, the sole biotechnologically relevant organism in the Tremellomycete family. First, we sequence the genome. Then, we perform transcriptomics under a variety of conditions, focusing on light and oxidative stress. This data not only reveals novel photobiology and metabolic regulation, it also allows derivation of constitutive and regulated gene expression parts. Our analysis ofX. dendrorhousphotobiology shows for the first time that a complex system of white-collar and cryptochrome homologs mediate response to ultraviolet light (UV). Our analysis of metabolic regulation shows that UV activates DNA repair, aromatic amino acid and carotenoid biosynthesis and represses central carbon metabolism and the fungal-like apoptotic pathway. Thus,X. dendrorhousshows a dynamic response toward biosynthetic pathways for light-absorbing compounds and survival and away from energy production. We then define a modular cloning system, including antibiotic selections, integration sites, and reporter genes, and use the transcriptomics to derive strong constitutive and regulated promoters. Notably, we discover a novel promoter from a hypothetical gene that has 9-fold activation upon UV exposure. Thus, -omics-to-parts workflows can simultaneously provide useful genomic data and advance genetic tools for non-model microbes, particularly those without a closely related model organism. This approach will be broadly useful in current efforts to engineer diverse microbes.

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bli-4

Cited by 2 publications

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Reactive Oxygen Species Affect Photomorphogenesis in Neurospora crassa

Reactive Oxygen Species Affect Photomorphogenesis in Neurospora crassa

Omics driven onboarding of the carotenoid producing red yeastXanthophyllomyces dendrorhousCBS 6938

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