This paper presents a comparison between two blind source separation methods: the first one based on a microcontroller board such as Arduino Due by the RTW (Real-Time Workshop) integrated into Matlab Simulink and the second one based on a DSP board (TMS320c6713). This study compared the space occupied, the execution speed and the separation index between the two platforms Arduino and DSP. The separation was practically simulated on the Arduino Due and TMS320c6713 board by two signal generators and an oscilloscope.