Block copolymers with a polyvinyl and a polypeptide block: factors governing the folding of the polypeptide chains

Douy, André; Gallot, Bernard

doi:10.1016/0032-3861(82)90405-0

Cited by 81 publications

(94 citation statements)

References 13 publications

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“…6,7,[9][10][11][12][13][14] Peptides or proteins have also been used as one block of block copolymers and shown to self-assemble into ordered structures. [15][16][17][18][19] Biomolecules are generally believed to be good candidates for nanotechnology due to their versatility, small size and precise control over self-assembly. In the context of block copolymers, incorporation of biomolecules heralds a new domain of novel functional materials.…”

Section: Introductionmentioning

confidence: 99%

Protein nanorings organized by poly(styrene-block-ethylene oxide) self-assembled thin films

et al. 2015

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This study explores the use of block copolymer self-assembly to organize Lsmα, a protein which forms stable doughnut-shaped heptameric structures. Here, we have explored the idea that 2-D crystalline arrays of protein filaments can be prepared by stacking doughnut shaped Lsmα protein into the poly(ethylene oxide) blocks of a hexagonal microphase-separated polystyrene-b-polyethylene oxide (PS-b-PEO) block copolymer. We were able to demonstrate the coordinated assembly of such a complex hierarchical nanostructure. The key to success was the choice of solvent systems and protein functionalization that achieved sufficient compatibility whilst still promoting assembly. Unambiguous characterisation of these structures is difficult; however AFM and TEM measurements confirmed that the protein was sequestered into the PEO blocks. The use of a protein that assembles into stackable doughnuts offers the possibility of assembling nanoscale optical, magnetic and electronic structures. This study explores the use of block copolymer self-assembly to organize Lsmα, a protein which forms stable doughnut-shaped heptameric structures. Here, we have explored the idea that 2-D crystalline arrays of protein filaments can be prepared by stacking doughnut shaped Lsmα protein into the poly(ethylene oxide) blocks of a hexagonal microphase-separated polystyrene-b-polyethylene oxide (PSb-PEO) block copolymer. We were able to demonstrate the coordinated assembly of such a complex hierarchical nanostructure. The key to success was the choice of solvent systems and protein functionalization that achieved sufficient compatibility whilst still promoting assembly. Unambiguous characterisation of these structures is difficult; however AFM and TEM measurements confirmed that the protein was sequestered into the PEO blocks. The use of a protein that assembles into stackable doughnuts offers the possibility of assembling nanoscale optical, magnetic and electronic structures.

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Section: Introductionmentioning

confidence: 99%

Protein nanorings organized by poly(styrene-block-ethylene oxide) self-assembled thin films

et al. 2015

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“…2 For example, poly(styrene)-block-poly(γ-benzyl-L-glutamate) (PS-block-PBLG) block copolymers exhibit lamellar mesophases in the solid state, and each sheet of the lamellae is composed of two layers, disordered PS layer and the polypeptide layer arranged in an α-helix conformation. 3 Some morphologies with fine structures were also observed in the synthetic hybrid materials. It was demonstrated that poly(Z-L-lysine)-block-polystyrene rod-coil block copolymers selfassemble into hexagonal-in-zigzag lamellae structures in bulk.…”

Section: Introductionmentioning

confidence: 88%

Brush macromolecules with thermo-sensitive coil backbones and pendant polypeptide side chains: synthesis, self-assembly and functionalization

2015

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Macromolecular brushes with thermo-sensitive poly(N-isopropylacrylamide) (PNIPAM) backbones and pendant poly(γ-benzyl-L-glutamate) (PBLG) side chains were synthesized by reversible addition-fragmentation chain transfer (RAFT) and ring-opening polymerization (ROP). Copolymers of NIPAM and N-acryloxysuccinimide (NAS) were synthesized by RAFT polymerization. Boc-protected amino groups were introduced to the backbone chains by substituting the NAS groups with N-Boc-ethylenediamine, and after removal of the Boc groups amine groups were produced on the backbones. PBLG side chains were synthesized by ROP. Size exclusion chromatograph, 1 H NMR and 13 C NMR results all indicated successful synthesis of well-defined brush macromolecules. The self-assembly of the macromolecules in aqueous solutions was investigated in this research. Depending on the backbone and side chain lengths, the brush macromolecules self-assemble into spherical micelles, worm-like structures and vesicles. Temperature exerted a significant effect on the morphology of the structures. At a temperature above lower critical temperature (LCST) of PNIPAM backbone, the selfassembled structures aggregated together. The functionalization of PBLG side chains with 1-pyrenebutyric acid was also performed. The fluorescence properties and morphology of the functionalized brush macromolecules were investigated in this research.

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“…Gallot et al reported that depending on the casting solvent used, the helices of poly(␥-benzyl-l-glutamate) were either arranged in a two-dimensional hexagonal array (1,4-dioxane), or acquired a more complex morphology [26]. Minich et al reported that PBLG 75 racL 75 exhibited local columnar hexagonal packing coexisting with cholesteric organization [11].…”

Section: Solid-state Structurementioning

confidence: 98%

Solid-state structure of gelatin-mono epoxy terminated polydimethylsiloxane polymer: Effect of electrostatic and hydrophobic interactions

Xia

Qiao

et al. 2014

Colloids and Surfaces B: Biointerfaces

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Block copolymers with a polyvinyl and a polypeptide block: factors governing the folding of the polypeptide chains

Cited by 81 publications

References 13 publications

Protein nanorings organized by poly(styrene-block-ethylene oxide) self-assembled thin films

Protein nanorings organized by poly(styrene-block-ethylene oxide) self-assembled thin films

Brush macromolecules with thermo-sensitive coil backbones and pendant polypeptide side chains: synthesis, self-assembly and functionalization

Solid-state structure of gelatin-mono epoxy terminated polydimethylsiloxane polymer: Effect of electrostatic and hydrophobic interactions

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