Blockade of invasion and metastasis of breast cancer cells via targeting CXCR4 with an artificial microRNA

Liang, Zhongxing; Wu, Hui; Reddy, Santosh; Zhu, Aizhi; Wang, Sijia; Blevins, Dean; Yoon, Younghyoun; Zhang, Yawei; Shim, Hyunsuk

doi:10.1016/j.bbrc.2007.09.007

Cited by 115 publications

(77 citation statements)

References 22 publications

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“…Total RNA was extracted from the breast cancer cells and cultured for 48 h at 4˚C using TRIzol Reagent (Invitrogen; Thermo Fisher Scientific, Inc.) as previously described (20) and was treated with RNse-free Dnase I (Promega Corporation, Madison, WI, USA). The concentration and purity of the isolated RNA was measured with SMA 400 UV0VIS (Merinton Instrument, Ltd., Shanghai, China).…”

Section: Cellular Invasion Assaymentioning

confidence: 99%

Protective role of LRRC3B in preventing breast cancer metastasis and recurrence post-bupivacaine

Kong

Zou

2017

Oncology Letters

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Abstract. The present study aimed to investigate the potential effect of leucine-rich repeat containing 3B (LRRC3B) with respect to the inhibition of breast cancer recurrence and metastasis post-anesthesia. The mRNA expression of LRRC3B in breast MDA-MB-231 and MCF-7 cell lines was detected using reverse transcription-quantitative polymerase chain reaction (RT-qPCR) analysis. The effect of bupivacaine on breast cancer cell invasion was analyzed using a Matrigel assay. LRRC3B specific small interfering (si)RNA was constructed and transfected into breast cancer cells using Lipofectamine ® 2000 reagent. The influence of bupivacaine on LRRC3B expression was measured based on RT-qPCR. Additionally, the effect of LRRC3B silencing on the invasion of breast cancer cells treated with bupivacaine was analyzed. Compared with the control, LRRC3B expression significantly increased in MDA-MB-231 and in MCF-7 cells as the length of time increased (P<0.05), but the expression of the gene significantly declined in 2 types of cancer cell when the cells were transfected with siRNA-LRRC3B plasma (P<0.05). The administration of 50 µg/ml bupivacaine promoted maximum breast cancer cell invasion, and suppressed LRRC3B mRNA expression in cells. However, when LRRC3B was silenced in cancer cells, 20 µg/ml bupivacaine significantly promoted cancer cell invasion, indicating that bupivacaine suppresses the expression of LRRC3B and promotes cell invasion. The present study suggested that LRRC3B serves a protective role in preventing bupivacaine-induced breast cancer recurrence and metastasis.

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Section: Cellular Invasion Assaymentioning

confidence: 99%

Protective role of LRRC3B in preventing breast cancer metastasis and recurrence post-bupivacaine

Kong

Zou

2017

Oncology Letters

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“…miRNAs have profound positive and negative effects on cancer metastasis (18,19). Overexpression of an artificial miRNA that targeted the CXCR4 gene in the human breast cancer cell line, MDA-MB-231, decreases cell migration and invasion (18). miR-10b is highly expressed in human and mouse metastatic breast cancer cell lines and positively regulates cell migration and metastasis (19).…”

Section: Micrornas (Mirnas)mentioning

confidence: 99%

Section: Micrornas (Mirnas)mentioning

confidence: 99%

ST14 (Suppression of Tumorigenicity 14) Gene Is a Target for miR-27b, and the Inhibitory Effect of ST14 on Cell Growth Is Independent of miR-27b Regulation

Wang

Rathinam²,

Walch³

et al. 2009

Journal of Biological Chemistry

100

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MicroRNAs are noncoding, endogenous small RNAs that regulate target genes by cleavage of the targeted mRNA or translational repression. We investigated the microRNAome using 2-color microarrays in a highly invasive human breast cancer cell line, MDA-MB-231 (subline 4175) and a noninvasive breast epithelial cell line, MCF10A. We found 13 microRNAs that were up-regulated, and nine were down-regulated significantly in 4175 cells (p < 0.05, -fold change >2) compared with MCF10A cells. Interestingly, miR-27b and its putative target gene, ST14 (suppressor of tumorigenicity 14), had inverse expression pattern in breast cancer cells. The 3-untranslated region of ST14 contains a regulatory element for miR-27b, and our luciferase experiments indicate that antisense miR-27b enhances ST14 expression in cancer cells. Furthermore, antagomir of miR-27b suppressed cell invasion in 4175 cells, whereas pre-miR-27b stimulated invasion in moderately invasive ZR75 breast cancer cells. In addition, ST14 reduces cell proliferation as well as cell migration and invasion. Analysis of human breast tumors revealed that miR-27b expression increases during cancer progression, paralleling a decrease in ST14 expression. Furthermore, our data indicate that ST14 inhibits cells from entering into S phase by up-regulating p27, which results in down-regulation of cyclin E-CDK2 complexes, suggesting ST14 reduces cell growth through its effects on cell cycle-related proteins. Introduction of miR-27b into ST14-expressing cells did not suppress the effect on cell growth. These findings suggest that ST14 plays an important role in several biological processes, and some effects are not completely dependent on miR-27b regulation.

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“…Inhibition of chemokine (C-X-C motif) receptor 4 (CXCR4) with siRNA inhibited metastasis of breast cells in vivo (Liang et al, 2004(Liang et al, , 2005. Therefore, researchers designed and synthesized a pre-miRNA with an miR-155 backbone that targeted CXCR4 in order to determine whether the CXCR4/SDF-1 pathway was regulated by expression of miRNAs (Liang et al, 2007). After transfecting breast tumor cells with the synthesized miRNA, they observed a significant decrease in CXCR4 in breast tumor cells and reduced migration and invasion.…”

Section: Synthetic Mirnas Targeting Genes Associated With Metastasismentioning

confidence: 99%

Advances in MicroRNAs: Implications for Gene Therapists

Marquez

McCaffrey

2008

Human Gene Therapy

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MicroRNAs (miRNAs) are a class of small regulatory RNAs that are thought to regulate the expression of as many as one-third of all human messenger RNAs (mRNAs). miRNAs are thought to be involved in diverse biological processes, including tumorigenesis. Analysis of miRNA levels may have diagnostic implications. Evidence shows that numerous viruses interact with the miRNA machinery, and that a number of viruses encode their own miRNAs. It seems likely that miRNAs will be implicated in many human diseases. Manipulation of miRNA levels by gene therapy provides an attractive new approach for therapeutic development. This review focuses on approaches to manipulate miRNA levels in cells and in vivo, and the implications for gene therapy. Furthermore, we discuss the use of endogenous miRNAs as scaffolds for the expression of RNA interference (RNAi) as well as competition between exogenous RNAi triggers and endogenous miRNAs. Because short interfering RNAs can also act as miRNAs, seed matches with the 3′ untranslated regions of genes should be avoided to prevent off-target effects. Last, we discuss the use of miRNAs to avoid immune responses to viral vectors.

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Blockade of invasion and metastasis of breast cancer cells via targeting CXCR4 with an artificial microRNA

Cited by 115 publications

References 22 publications

Protective role of LRRC3B in preventing breast cancer metastasis and recurrence post-bupivacaine

Protective role of LRRC3B in preventing breast cancer metastasis and recurrence post-bupivacaine

ST14 (Suppression of Tumorigenicity 14) Gene Is a Target for miR-27b, and the Inhibitory Effect of ST14 on Cell Growth Is Independent of miR-27b Regulation

Advances in MicroRNAs: Implications for Gene Therapists

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