Blockage of AM V reverse transcriptase by antisense oligodeoxynucleotides

doi:10.1016/0014-5793(90)81327-k

Cited by 7 publications

(1 citation statement)

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“…The longer extension product most likely reflects the correct transcription start site based on the following arguments. First, it is known that AMV reverse transcriptase prematurely terminates cDNA synthesis when reaching a stem loop in the RNA, and that the prematurely terminated molecules map at the bottom of the secondary structure [71] , [72] . The position of the strong signal coincides with the 3′ end of an inverted repeat (indicated in Fig.…”

Section: Resultsmentioning

confidence: 99%

A Complex Genetic Switch Involving Overlapping Divergent Promoters and DNA Looping Regulates Expression of Conjugation Genes of a Gram-positive Plasmid

et al. 2014

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Plasmid conjugation plays a significant role in the dissemination of antibiotic resistance and pathogenicity determinants. Understanding how conjugation is regulated is important to gain insights into these features. Little is known about regulation of conjugation systems present on plasmids from Gram-positive bacteria. pLS20 is a native conjugative plasmid from the Gram-positive bacterium Bacillus subtilis. Recently the key players that repress and activate pLS20 conjugation have been identified. Here we studied in detail the molecular mechanism regulating the pLS20 conjugation genes using both in vivo and in vitro approaches. Our results show that conjugation is subject to the control of a complex genetic switch where at least three levels of regulation are integrated. The first of the three layers involves overlapping divergent promoters of different strengths regulating expression of the conjugation genes and the key transcriptional regulator RcoLS20. The second layer involves a triple function of RcoLS20 being a repressor of the main conjugation promoter and an activator and repressor of its own promoter at low and high concentrations, respectively. The third level of regulation concerns formation of a DNA loop mediated by simultaneous binding of tetrameric RcoLS20 to two operators, one of which overlaps with the divergent promoters. The combination of these three layers of regulation in the same switch allows the main conjugation promoter to be tightly repressed during conditions unfavorable to conjugation while maintaining the sensitivity to accurately switch on the conjugation genes when appropriate conditions occur. The implications of the regulatory switch and comparison with other genetic switches involving DNA looping are discussed.

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Section: Resultsmentioning

confidence: 99%