Blockage of ovulation in the explanted hamster ovary by a collagenase inhibitor

Ichikawa, S.; Ohta, Michio; Morioka, Hiroshi; Murao, Sawao

doi:10.1530/jrf.0.0680017

Cited by 35 publications

(13 citation statements)

References 11 publications

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“…Moreover, many investigators showed that the administration (Ichikawa et al, 1983;Reich et al, 1985;Brännström et al, 1988 (Okuda et al, 1980). In addition, we have demonstrated that the injected anticoagulants reduced the number of the ovulated ova in PMSG-hCG treated immature rats (Iwamasa et al, 1991).…”

Section: Discussionmentioning

confidence: 99%

Association of immunolocalization of matrix metalloproteinase 1 with ovulation in hCG-treated rabbit ovary

Tadakuma¹,

Okamura²,

Kitaoka³

et al. 1993

Reproduction

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Section: Discussionmentioning

confidence: 99%

Association of immunolocalization of matrix metalloproteinase 1 with ovulation in hCG-treated rabbit ovary

Tadakuma¹,

Okamura²,

Kitaoka³

et al. 1993

Reproduction

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“…Tunica/thecal fibroblasts and follicular steroidogenic (theca, granulosa) cells are sources of procollagenases [29,30]. General chemical inhibitors of collagenases suppressed ovulation (hamster, rat) in vitro and in vivo [31][32][33][34]. Mice with a mutation in the type I collagen gene, conferring resistance to collagenase, are infertile (presumably due to anovulation) [35].…”

Section: Ovulationmentioning

confidence: 99%

Proteolytic Mechanisms in the Ovulatory Folliculo-Luteal Transformation

Murdoch

Gottsch²

2003

Connective Tissue Research

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The connective tissue matrix of the wall of ovarian follicles is degraded and remodeled during ovulatory rupture and formation of the corpus luteum. Ovarian surface epithelial cells in close contact with the apical wall of preovulatory ovine follicles secrete a urokinase-type plasminogen activator in response to surge levels of gonadotropins. Urokinase activates latent collagenases and stimulates release of tumor necrosis factor alpha from thecal endothelium. Tumor necrosis factor alpha progressively induces matrix metalloproteinase gene expression, apoptosis, and inflammatory necrosis. Collagenolysis and cellular death are a prelude to stigma formation and ovarian rupture. Ovulation is blocked by intrafollicular injection of TNFalpha or MMP-2 antisera. Unruptured follicles luteinize but are deficient in collagenous/vascularized trabeculae, and produce less progesterone than their control luteal counterparts. It appears that TNFalpha, via MMP-2 induction, contributes to the reorganization of an ovulatory follicle into a fully competent corpus luteum.

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“…These enzymes have been postulated to degrade the apical follicular connective tissue, resulting in a weakening of the follicular wall, thus facilitating oocyte release [4,5]. In support of this hypothesis are the reports that the activity of ovarian collagenase, gelatinase, and proteoglycanase increase during the preovulatory period [6-101 and that administration of metalloproteinase inhibitors results in an inhibition of ovulation [11,12].…”

Section: Introductionmentioning

confidence: 99%

Collagenase, Gelatinase, and Proteoglycanase Messenger Ribonucleic Acid Expression and Activity during Luteal Development, Maintenance, and Regression in the Pseudopregnant Rat Ovary1

Nothnick

Keeble

Curry

1996

Biology of Reproduction

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In the present study, we examined the roles of the matrix metalloproteinases collagenase, 72-kDa and 92-kDa gelatinase, and proteoglycanase in the tissue remodeling that occurs during luteal development and regression, using a pseudopregnant rat model. Pseudopregnancy was induced in immature female rats by eCG/hCG priming, and animals (n = 3 to 4 per time point) were killed on Days 1, 2, 4, 8, 12, 14, or 16 of pseudopregnancy (Day 0 = time of hCG administration). Ovaries were then removed and analyzed for either matrix metalloproteinase mRNA expression or activity. During luteal development (Day 1 of pseudopregnancy), activity of both collagenase (p = 0.009) and gelatinase (p = 0.0003), but not proteoglycanase (p > or = 0.05), was significantly greater than at all other time points. In accord with gelatinase activity, transcript levels of this enzyme were elevated at Day 1 of pseudopregnancy. Specifically, gelatinase transcript levels for the 72-kDa and 92-kDa enzymes were greatest at Day 1 (p = 0.0003 and p = 0.001, respectively), decreased 3-fold by Day 2,4-fold by Day 4, and reached 10-fold lower levels by Days 8 and 12 of pseudopregnancy. Proteoglycanase transcript could not be detected by Northern analysis during luteal development or any other time point examined in the current study. During the period of luteal maintenance (approximately Days 4-8 of pseudopregnancy in the rat), collagenase and gelatinase displayed basal levels of activity, but only proteoglycanase activity was elevated compared to luteal development levels of this enzyme. During luteal regression (Days 12-14 of pseudopregnancy in the rat), all enzymes displayed basal levels of enzyme activity. In accord with gelatinolytic activity during luteal regression, both 72-kDa and 92-kDa gelatinase mRNA were detectable at baseline levels. In contrast to the baseline levels of collagenolytic activity during luteal regression, collagenase transcript displayed peak values (approximately 8-fold greater than Day 1 levels; p = 0.004) at Day 12 of pseudopregnancy. It is concluded from these studies that collagenase and the gelatinases play a role in the tissue remodeling associated with luteal development, proteoglycanase is associated with luteal maintenance, and collagenase may contribute to the structural regression of the corpus luteum.

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Blockage of ovulation in the explanted hamster ovary by a collagenase inhibitor

Cited by 35 publications

References 11 publications

Association of immunolocalization of matrix metalloproteinase 1 with ovulation in hCG-treated rabbit ovary

Association of immunolocalization of matrix metalloproteinase 1 with ovulation in hCG-treated rabbit ovary

Proteolytic Mechanisms in the Ovulatory Folliculo-Luteal Transformation

Collagenase, Gelatinase, and Proteoglycanase Messenger Ribonucleic Acid Expression and Activity during Luteal Development, Maintenance, and Regression in the Pseudopregnant Rat Ovary1

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