Blocking histone deacetylation in Arabidopsis induces pleiotropic effects on plant gene regulation and development

Tian, Lining

doi:10.1073/pnas.011347998

Cited by 108 publications

(103 citation statements)

References 5 publications

(6 reference statements)

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“…Previous reports have shown that expression of HDA6 in other plant species such as Arabidopsis is higher in reproductive tissues [3, 40]. Consistent with these reports, differential expression of HDA6 in five tissues of common bean showed higher expression in the flowers and pod as shown in Figure 4.…”

Section: Discussionsupporting

confidence: 86%

“…There is considerable evidence showing that HDACs regulate transcription and play significant roles in various plant developmental processes and response to environmental factors [40, 41]. To identify if the HDA6 expression varies with plant age, we determined its expression profile at three developmental stages (seedling, flowering, and pod) using the quantitative PCR.…”

Section: Discussionmentioning

confidence: 99%

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Characterization and Expression Analysis of Common BeanHistone Deacetylase 6during Development and Cold Stress Response

Hayford

Ligaba-Osena

Subramani

et al. 2017

International Journal of Genomics

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Histone deacetylases (HDACs) are important regulators of gene transcription thus controlling multiple cellular processes. Despite its essential role in plants, HDA6 is yet to be validated in common bean. In this study, we show that HDA6 is involved in plant development and stress response. Differential expression of HDA6 was determined in various tissues and the expression was seen to be upregulated with plant age (seedling < flowering < maturity). Higher expression was observed in flowers and pods than in stem, leaf, and root. Upregulation of HDA6 gene during cold stress implies its prominent role in abiotic stress. Furthermore, the HDA6 gene was isolated from three common bean genotypes and sequence analyses revealed homology with functionally characterized homologs in model species. The 53 kDa translated product was detected using an HDA6 specific antibody and recombinant protein overexpressed in Escherichia coli showed HDAC activity in vitro. To our knowledge, this is the first report in the agriculturally important crop common bean describing the functional characterization and biological role of HDA6.

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Section: Discussionsupporting

confidence: 86%

Section: Discussionmentioning

confidence: 99%

Characterization and Expression Analysis of Common BeanHistone Deacetylase 6during Development and Cold Stress Response

Hayford

Ligaba-Osena

Subramani

et al. 2017

International Journal of Genomics

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“…[5][6][7][8] Another RPD3-type HDAC, HDA19, was found to be a global modulator of gene expression in plant development and stress response. [9][10][11] More recently, we found that HDA1type HDACs, HDA5, HDA8 and HDA14, were localized in cytoplasm, suggesting their potential for nuclear transport and deacetylatingorganellar and cytoplasmic proteins. 12 HD2 proteins are plant specific HDACs which were originally discovered in maize.…”

Section: Histone Deacetylases In Arabidopsismentioning

confidence: 98%

HD2 proteins interact with RPD3-type histone deacetylases

Luo

Wang

Liu

et al. 2012

Plant Signaling & Behavior

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HD2 proteins were previously identified as plant specific histone deacetylases (HDACs). The molecular mechanism of the function of HD2 proteins is still unclear. Using Bimolecular fluorescence complementation assay, we demonstrated that Arabidopsis HD2 proteins, HD2A, HD2C and HD2D, can interact with RPD3-type HDACs, HDA6 and HDA19, suggesting that that these proteins may act in the same protein complex. Our study indicates that HD2 proteins may functionally associate with RPD3-type HDACs to regulate gene expression in plants.

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“…Another mutant showed moderately serrated leaves (Fig. 3B), which could be caused by overexpression of a zinc-finger protein (Prigge and Wagner 2001), cytokinin (Greenboim-Wainberg et al 2005), or reduction of a histone deacetylase (AtHD1) expression (Tian and Chen 2001), to name just a few possibilities. Both mutants are sterile.…”

Section: Spectrum Of Mutationsmentioning

confidence: 99%

Generation of Arabidopsis Mutants by Heterologous Expression of a Full-Length cDNA Library from Tomato Fruits

et al. 2009

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Heterologous expression of cDNA library in Arabidopsis and other plants has been used for gene identifications. To identify functions of tomato genes, we expressed a tomato full-length cDNA library in Arabidopsis thaliana and generated over 7,000 mutants. We constructed a tomato cDNA library with a plant transformation-ready binary vector that contained a higher percentage of fulllength cDNAs since synthesized double-stranded cDNA was size-selected using gel electrophoresis, with cDNA sizes of 2-5 kb being gel-purified for ligation onto the binary vector. Sequencing of 81 cDNA clones indicates that 75% (61) are full-length genes, which is similar to sequencing of inserted cDNA in Arabidopsis. The library was used to transform Arabidopsis plants. Among the 7,000 mutants, one was found to be a dwarf due to the expression of an ATP synthase, and another vegetative mutant did not produce flowers even after 7 months. The technique was validated by reintroducing the tomato ribosomal protein L9 gene and can be used in any other plant species as a gene discovery tool.

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Blocking histone deacetylation in Arabidopsis induces pleiotropic effects on plant gene regulation and development

Cited by 108 publications

References 5 publications

Characterization and Expression Analysis of Common BeanHistone Deacetylase 6during Development and Cold Stress Response

Characterization and Expression Analysis of Common BeanHistone Deacetylase 6during Development and Cold Stress Response

HD2 proteins interact with RPD3-type histone deacetylases

Generation of Arabidopsis Mutants by Heterologous Expression of a Full-Length cDNA Library from Tomato Fruits

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