Blocking lncRNA MIR155HG/miR-155-5p/-3p inhibits proliferation, invasion and migration of clear cell renal cell carcinoma

Tao, Meiman; Zhou, Yang; Jin, Yichen; Pu, Jinxian

doi:10.1016/j.prp.2019.152803

Cited by 15 publications

(12 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the present study, miR-155-5p was a target of miR155HG and inversely correlated with miR155HG expression, suggesting that miR155HG may influence OC by regulating miR-155-5p. In fact, miR-155-5p is downregulated and acts as a target of miR155HG in some cancers, such as glioma (37), laryngeal squamous cell carcinoma (24) and clear cell renal cell carcinoma (38). In the present study, miR-155-5p was downregulated in OC, indicating that miR-155-5p may be an anti-oncogene.…”

Section: Discussionsupporting

confidence: 49%

Long non‑coding RNA miR155HG silencing restrains ovarian cancer progression by targeting the microRNA‑155‑5p/tyrosinase‑related protein 1 axis

Wen

Luo

et al. 2021

Exp Ther Med

View full text Add to dashboard Cite

Ovarian cancer (OC) is the third commonest gynecological malignancy worldwide. The long non-coding (lnc)RNA microRNA (miR)155HG functions as an oncogene in different human cancers. However, the function and molecular mechanism of miR155HG in OC remain elusive. The present study indicated that the expression levels of miR155HG and tyrosinase-related protein 1 (TYRP1) were significantly increased, whereas that of miR155-5p was decreased in OC tissues and cells, as detected by real-time quantitative polymerase chain reaction. It was demonstrated that knockdown of miR155HG markedly inhibited OC cell viability, migration and invasion while promoting apoptosis, as indicated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, wound healing, Transwell and western blot assays. Mechanistically, it was revealed that miR155HG and TYRP1 were both targeted by miR-155-5p with complementary binding sites in the 3' untranslated region. A dual-luciferase reporter assay was used to confirm the targeting relationship between miR155HG, miR-155-5p and TYRP1. In addition, the interaction between miR155HG and miR-155-5p was further demonstrated by radioimmunoprecipitation and pull-down assays. In addition, feedback approaches determined that miR-155-5p inhibition or TYRP1 overexpression markedly reversed the inhibitory effects of miR155HG knockdown on OC cell viability, migration and invasion as well as weakened the promotive effect of miR155HG knockdown on OC cell apoptosis. Thus, miR155HG silencing inhibited the malignant biological behavior of OC cells by targeting the miR-155-5p/TYRP1 axis. The present study provides novel insights into the underlying mechanism of OC progression.

show abstract

Section: Discussionsupporting

confidence: 49%

Long non‑coding RNA miR155HG silencing restrains ovarian cancer progression by targeting the microRNA‑155‑5p/tyrosinase‑related protein 1 axis

Wen

Luo

et al. 2021

Exp Ther Med

View full text Add to dashboard Cite

show abstract

“…Meanwhile, we presented that inhibition of miR-155-3p reduced proliferation, colony formation, invasion and migration abilities and promoted apoptosis of MB cells in vitro. According to a report by Tao et al it is suggested that silencing of miR-155-3p restrains the proliferation, invasion and migration of clear cell renal cell carcinoma cells [ 30 ]. Moreover, it has been specified that miR-155-3p up-regulation in breast cancer cells blocks cellular apoptosis [ 31 ].…”

Section: Discussionmentioning

confidence: 99%

microRNA-155-3p delivered by M2 macrophages-derived exosomes enhances the progression of medulloblastoma through regulation of WDR82

Luan

et al. 2022

J Transl Med

View full text Add to dashboard Cite

Objective Exosomes, membranous nanovesicles, naturally bringing proteins, mRNAs, and microRNAs (miRNAs), play crucial roles in tumor pathogenesis. This study was to investigate the role of miR-155-3p from M2 macrophages-derived exosomes (M2-Exo) in promoting medulloblastoma (MB) progression by mediating WD repeat domain 82 (WDR82). Methods miR-155-3p expression was detected by RT-qPCR. The relationship of miR-155-3p with clinicopathological features of MB patients was analyzed. M2-Exo were isolated and identified by TEM, NTA and Western blot. CCK-8 assay, colony formation assay, flow cytometry, wound healing assay, and Transwell assay were performed to explore the role of miR-155-3p-enriched M2-Exo on the progression of MB cells. Luciferase assay were used to identify the relationship between miR-155-3p and WDR82. The effect of miR-155-3p-enriched M2-Exo on tumorigenesis of MB was confirmed by the xenograft nude mice model. Results miR-155-3p was up-regulated in MB tissues of patients and MB cell lines. High miR-155-3p expression was correlated with the pathological type and molecular subtype classification of MB patients. WDR82 was a direct target of miR-155-3p. miR-155-3p was packaged into M2-Exo. miR-155-3p-enriched M2-Exo promoted the progression of Daoy cells. miR-155-3p-enriched M2-Exo promoted in vivo tumorigenesis. Conclusion The study highlights that miR-155-3p-loaded M2-Exo enhances the growth of MB cells via down-regulating WDR82, which might provide a deep insight into MB mechanism.

show abstract

“…In contrast, inhibition of miR-155-5p and miR-155-3p inhibited the proliferation, invasion, and migration of ccRCC, providing a new method for early diagnosis and proper treatment of ccRCC ( 37 ). Yamada et al reported that miR-144-5p and carcinogenic SDC3 axis were closely related to the pathogenesis of RCC and played antitumor roles ( 38 ).…”

Section: Discussionmentioning

confidence: 99%

Construction of five microRNAs prognostic markers and a prognostic model for clear cell renal cell carcinoma

Zhou¹,

Zhang²,

Ang³

et al. 2021

Transl Cancer Res TCR

View full text Add to dashboard Cite

Background: To determine the role of miRNA in the progression and outcome of renal clear cell carcinoma (ccRCC), establish a model for predicting outcome in patients with ccRCC and verify it using a Cox regression model. The miRNA target genes were predicted to understand their biological functions.Methods: The microRNAs of 71 normal tissues and 545 tumor tissues were downloaded from TCGA (https://tcga-data.nci.nih.gov/tcga/). We also downloaded 537 clinical materials from this website. The miRNA difference analysis was carried out. A prognostic model was constructed using differential miRNA.The model was verified using Cox survival analysis, receiver operator characteristic (ROC), and independent predictive analysis.Results: MiR-130b-3p, miR-365b-3p, miR-149-5p, miR-155-5p, and miR-144-5p can be used as independent prognostic indicators. We also analyzed the related functions of the target gene and found that target genes of miRNAs are involved in the signal pathways of some tumors, including cholesterol metabolism, HIF-1 signal pathway, focus adhesion, the Rap1 signal pathway, and hepatitis C. Conclusions:The prognostic model constructed using five miRNAs is an independent and accurate factor. These miRNAs target genes are involved in regulating a variety of tumorigenesis and signal pathways.Therefore, we have reason to believe that the regulation of signal pathways by miRNA may play a critical role in the occurrence, development, and outcome of ccRCC, provide a new therapeutic target for ccRCC, and improve outcomes.

show abstract

Blocking lncRNA MIR155HG/miR-155-5p/-3p inhibits proliferation, invasion and migration of clear cell renal cell carcinoma

Cited by 15 publications

References 28 publications

Long non‑coding RNA miR155HG silencing restrains ovarian cancer progression by targeting the microRNA‑155‑5p/tyrosinase‑related protein 1 axis

Long non‑coding RNA miR155HG silencing restrains ovarian cancer progression by targeting the microRNA‑155‑5p/tyrosinase‑related protein 1 axis

microRNA-155-3p delivered by M2 macrophages-derived exosomes enhances the progression of medulloblastoma through regulation of WDR82

Construction of five microRNAs prognostic markers and a prognostic model for clear cell renal cell carcinoma

Contact Info

Product

Resources

About