Blocking long noncoding RNA MALAT1 restrained the development of laryngeal and hypopharyngeal carcinoma

Abstract

Purpose The long non-coding RNA MALAT1 is a predictive marker in several solid tumors with highly conserved sequences. However, the role of non-coding RNA in development of laryngeal or hypopharyngeal cancer remains unclear. Methods Tumor tissues and adjacent non-cancer tissues of 24 patients were collected. We detected the expression of MALAT1 in laryngeal cancer tissues and hypopharyngeal cancer tissues. Moreover, we developed a MALAT1 silencing model in human laryngeal tumor cells by transfecting MALAT1 small interfering RNA into human laryngeal carcinoma cell line Hep-2 and pharyngeal carcinoma cell line FaDu with Lipofectamine 2000 system. Cell cycle analysis, Cell Counting Kit-8 assay, Transwell assay, quantitative reverse transcription PCR, and wound-healing assays were performed to evaluate the impact of MALAT1 depletion on laryngeal or hypopharyngeal cancer cell's growth, proliferation, apoptosis, invasion and migration. Results MALAT1 was significantly up-regulated in laryngeal and hypopharyngeal carcinoma cells. MALAT1 down-regulation induced the increased apoptosis of both cell lines and suppressed cells' proliferation. Cells were arrested in G1/G2 phase and cells of S phase were significantly decreased. Down-regulation of MALAT1 expression can also inhibit the migration and invasion of laryngeal squamous cell carcinoma cell (Hep-2) and hypopharyngeal cancer cell (FaDu). Conclusion In summary, our deactivation model of MALAT1 disentangled the active function of it as a regulator of gene expression governing the hallmarks of laryngeal and hypopharyngeal cancer. Blocking this long non-coding RNA may restrain the development of laryngeal cancer.

The Role and Mechanism of MALAT1 Long Non-Coding RNA in the Diagnosis and Treatment of Head and Neck Squamous Cell Carcinoma

“…MALAT1 has a regulatory effect on the occurrence and development of several tumors, including laryngeal, hypopharyngeal, thyroid, esophageal, lung, liver, and ovarian cancers. [30][31][32][33][34][35][36] The Relationship Between MALAT1 and HNSCC MALAT1 can regulate the occurrence and development of a variety of malignant tumors. It also plays roles in transcription, cell cycle regulation, epigenetics, and tumor metastasis.…”

“…44 Inactivation of MALAT1 can inhibit gene expression regulators that modify the characteristics of laryngeal cancer and hypopharyngeal carcinoma, and blocking MALAT1 expression can inhibit the progression of both types of cancer. 33 lncRNA MALAT1 can trigger LSCC resistance to chemotherapy drugs by promoting tumor cell metastasis and inhibiting apoptosis. 43 lncRNA MALAT1 serves as a miR-429 sponge, regulating ZEB1 expression.…”

Regulatory Mechanisms of lncRNAs and Their Target Gene Signaling Pathways in Laryngeal Squamous Cell Carcinoma

“…Silencing MALAT1 in human laryngeal carcinoma cell line HEp-2 and pharynx cancer cell line FaDu (RRID: CVCL_1218) can induce apoptosis and inhibit cell proliferation, resulting in cell cycle arrest in the G1/G2 phase and significant relief in the S phase. Downregulation of MALAT1 expression can also inhibit the migration and invasion of HEp-2 and FaDu (Xu E. et al, 2019). Jiang et al reported that high expression of MALAT1 can enhance the invasive ability of Tu 686 and LSC-1 (RRID: CVCL_9U29) cells, considerably improve the survival rate, facilitate proliferation, and inhibit apoptosis.…”

“…Silencing MALAT1 in human laryngeal carcinoma cell line HEp-2 and pharynx cancer cell line FaDu (RRID: CVCL_1218) can induce apoptosis and inhibit cell proliferation, resulting in cell cycle arrest in the G1/G2 phase and significant relief in the S phase. Downregulation of MALAT1 expression can also inhibit the migration and invasion of HEp-2 and FaDu ( Xu E. et al., 2019 ). Jiang et al.…”

SERS Based Lateral Flow Assay for Rapid and Ultrasensitive Quantification of Dual Laryngeal Squamous Cell Carcinoma-Related miRNA Biomarkers in Human Serum Using Pd-Au Core-Shell Nanorods and Catalytic Hairpin Assembly

“…Laryngeal squamous cell carcinoma (LSCC), a common clinical malignancy of the head and neck, is a type of tumor that arises mainly from the mucosal epithelium of the larynx, accounting for approximately 5% of systemic tumors and 15% of head and neck tumors worldwide (Steuer et al, 2017;Xu et al, 2020). The main reason for shallow low 5-year survival rate, less than 50% in advanced stages of the disease, is the late diagnosis (Gourin et al, 2009;Adam et al, 2012;Brandstorp-Boesen et al, 2017).…”