Blood

Scanes, Colin G.

doi:10.1016/b978-0-12-407160-5.00010-5

Cited by 41 publications

(29 citation statements)

References 197 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For example, Bowes et al (1989) ) in broiler chickens. In addition, mean BUN concentration is reported to be 3.11 mg · dl −1 in avian plasma (Scanes, 2015). It is not clear why the values for GOT activity obtained in this study are far lower than those published elsewhere (Bowes et al, 1989).…”

Section: Discussioncontrasting

confidence: 54%

Effects of dietary <i>Corynebacterium ammoniagenes</i>-derived single cell protein on growth performance, blood and tibia bone characteristics, and meat quality of broiler chickens

An¹,

Choi²,

Kang³

et al. 2018

J. Anim. Feed Sci.

View full text Add to dashboard Cite

Section: Discussioncontrasting

confidence: 54%

Effects of dietary <i>Corynebacterium ammoniagenes</i>-derived single cell protein on growth performance, blood and tibia bone characteristics, and meat quality of broiler chickens

An¹,

Choi²,

Kang³

et al. 2018

J. Anim. Feed Sci.

View full text Add to dashboard Cite

“…(), an early increase of circulating heterophils indicates acute inflammation from 8 to 24 h p. inj . This reciprocal relationship between heterophil and lymphocyte proportions is frequently seen in immunologically stressed chickens (Scanes, ). Gross and Siegel () have defined this ratio as reliable and accurate physiological indicator for avian stress response and indicated H/L ratios equal or higher than 0.8 as high‐degree stress.…”

Section: Discussionmentioning

confidence: 80%

Haematological and febrile response to Escherichia coli lipopolysaccharide in 12‐week‐old cockerels of genetically diverse layer lines fed diets with increasing L‐arginine levels

Lieboldt

Frahm

Halle

et al. 2016

Animal Physiology Nutrition

View full text Add to dashboard Cite

Due to its decisive function in the avian metabolic, endocrine and immune system L-arginine (Arg) is dietary indispensable for chickens. In 12-week-old cockerels of two high- and two low-performing purebred layer lines, the effects of increasing dietary Arg on the haematological and febrile response were studied over 48 h after single lipopolysaccharide (LPS) injection. The offered diets contained Arg equivalent to 70%, 100% and 200% of recommended supply. Pathophysiological alterations in weight gain, feed intake, body temperature and differential blood count were examined in comparison with their physiological initial values. Within the first 24 h after LPS injection, cockerels reduced feed intake and lost body weight subsequently. Thereby, low-performing genotypes lost body weight to a lesser extent than high-performing ones. The loss of body weight was further intensified by deficient dietary Arg. Within the following 24 h, cockerels recovered by improving feed intake and weight gain. Furthermore, LPS induced genotype-specific fever response: both brown genotypes showed initial hypothermia followed by longer lasting moderate hyperthermia, whereas the white genotypes exhibited biphasic hyperthermia. Fever response was accompanied by significant changes in differential blood counts. Characterized by lymphopenia and heterophilia, a severe leucopenia was observed from 4 to 8 h after LPS injection and replaced by a marked leucocytosis with longer lasting monocytosis up to 48 h after LPS injection. Under given pathophysiological conditions, deficiently Arg-supplied cockerels showed higher total leucocyte counts than adequately and excessively Arg-supplied cockerels. However, deficient and surplus dietary Arg tended to cause higher ratios between heterophils and lymphocytes. To conclude, present results confirmed that LPS induced numerous immunological changes in 12-week-old cockerels and emphasized that chicken's genotype is a source of variation to be considered for immunological studies. Deficient dietary Arg intensified acute changes in differential blood counts and weight gain during LPS-induced inflammation.

show abstract

“…These blood samples were stored on ice (<3 hr) and used to assess changes in DNA damage (comet assay), Cort (radioimmunoassay), and uric acid (fluorometric assay). Comet assays for all four‐time points were performed on whole blood immediately following the last (120 min) sample; note that while whole blood was used for this analysis, red blood cells are the most predominant cell type in avian blood (∼200 red blood cells: 1 leukocyte; Scanes, 2015) and, thus, measured DNA damage levels are representative of erythrocyte damage. Blood samples were then separated by centrifugation after which separate aliquots of plasma were frozen at −20°C to later test for Cort and uric acid.…”

Section: Methodsmentioning

confidence: 99%

Beyond corticosterone: The acute stress response increases DNA damage in house sparrows

et al. 2020

View full text Add to dashboard Cite

Although corticosterone (Cort) has been the predominant metric used to assess acute stress in birds, it does not always accurately reflect how an animal copes with a stressor. Downstream measurements may be more reliable. In the current study, we tested the hypothesis that acute increases in DNA damage could be used to assess stressor exposure. Studies have shown DNA damage increases in response to stress‐related hormones in vitro; however, this has not yet been thoroughly applied in wild animals. We exposed house sparrows (Passer domesticus) to a 30‐ or 120‐min restraint stressor and took blood samples at 0, 30, 60, and 120 min to measure Cort, DNA damage, and uric acid. Both treatments increased DNA damage and Cort, and decreased uric acid. It thus appears that DNA damage can reflect acute stressor exposure. To improve the usability of DNA damage as a metric for stress, we also tested the impacts of sample storage on DNA damage. Leaving red blood cells on ice for up to 24 hr, only slightly influenced DNA damage. Freezing blood samples for 1–4 weeks substantially increased DNA damage. These findings emphasize the importance of reducing variation between samples by assaying them together whenever possible. Overall, these results indicate that assessing DNA damage is a valid method of assessing acute stressor exposure that is suitable for both laboratory‐ and field‐based studies; however, additional research is needed on the molecular dynamics of nucleated red blood cells, including whether and how their DNA is repaired.

show abstract

Blood

Cited by 41 publications

References 197 publications

Effects of dietary <i>Corynebacterium ammoniagenes</i>-derived single cell protein on growth performance, blood and tibia bone characteristics, and meat quality of broiler chickens

Effects of dietary <i>Corynebacterium ammoniagenes</i>-derived single cell protein on growth performance, blood and tibia bone characteristics, and meat quality of broiler chickens

Haematological and febrile response to Escherichia coli lipopolysaccharide in 12‐week‐old cockerels of genetically diverse layer lines fed diets with increasing L‐arginine levels

Beyond corticosterone: The acute stress response increases DNA damage in house sparrows

Contact Info

Product

Resources

About